Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (7): 194-204.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1040
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LU Xin-hua(), SUN De-quan, ZHANG Xiu-mei()
Received:
2021-08-15
Online:
2022-07-26
Published:
2022-08-09
Contact:
ZHANG Xiu-mei
E-mail:sscrilu@163.com;asiazhang1975@catas.cn
LU Xin-hua, SUN De-quan, ZHANG Xiu-mei. Genetic Transformation of Plant Cells Mediated by Mesoporous Silica Nanoparticles[J]. Biotechnology Bulletin, 2022, 38(7): 194-204.
Fig.4 Agarose gel electrophoresis assay of Am-MSNs/DNA complexes A:Am-MSN-20/pDNA;B:Am-MSN-50/pDNA;1:free pDNA(positive CK);2:Am-MSNs(negative CK);3-8 presented that the mass ratios of Am-MSNs to pDNA are 5∶1,10∶1,20∶1,30∶1,40∶1 and 50∶1,respectively
Fig.5 Protection of Am-MSNs against pDNA digestion 1:free pDNA;2:free pDNA + DNase I;3:Am-MSN-20/pDNA;4:Am-MSN-20/pDNA + DNase I;5:Am-MSN-50/pDNA;6:Am-MSN-50/pDNA + DNase I
Fig.7 Am-MSN-mediated transient smGFP expression in the protoplasts of A. thaliana A:pDNA only;B:Am-MSNs;C:pDNA/PEG;D:pDNA/Am-MSN-20;E:pDNA/Am-MSN-50. Scale bars=5 µm
Fig.8 Assessment of smGFP gene expression in the proto-plasts of A. thaliana 48 h post-transformation The error line in the figure refers to the standard deviation. Different lowercase letters indicate a significant difference(P < 0.05)
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