Research Progress in Microfluidic Technology in the Detection of Pathogenic Microorganisms

doi:10.13560/j.cnki.biotech.bull.1985.2023-0479

Abstract

Abstract:

Rapid and accurate detection of pathogenic microorganisms is of great significance for epidemic prevention and control as well as the protection of people's lives and health. In recent years, researchers have developed a variety of technical methods for the detection of pathogenic microorganisms by reasonably designing microfluidic chips and combining microfluidic technology with various detection technologies. Compared with traditional pathogenic microorganism detection technology, microfluidic detection technology has outstanding advantages, i.e., low technical requirements for operators, less sample demand and high degree of automation, and is suitable for accurate and rapid detection of pathogenic microorganisms in various complex environments. In this paper, the application of microfluidic technology in the detection of pathogenic microorganisms such as viruses, bacteria, fungi, chlamydia and mycoplasma was reviewed, aiming to provide research ideas for the detection of pathogenic microorganisms, promote the development of microfluidic technology in the detection of pathogenic microorganisms, and improve the prevention and control ability of diseases.

Key words: pathogenic microorganisms, chip, microfluidics, automation

WAN Qi-wu, BAO Xu-dong, DING Ke, MOU Hua-ming, LUO Yang. Research Progress in Microfluidic Technology in the Detection of Pathogenic Microorganisms[J]. Biotechnology Bulletin, 2023, 39(10): 107-114.

Figures/Tables 3

Fig. 1 Application of microfluidic in the detection of pathogenic microorganisms

Fig. 2 Microfluidic system for virus detection a: Fully automated centrifugal microfluidic system for sample response viral nucleic acid detection[11]; b: a microfluidic system based on CRISPR diagnosis without instruments for SARS-CoV-2[12]; c: a self-powered fast-loading microfluidic chip using RT-LAMP to detect carrier viruses[13]

Fig. 3 Microfluidic system for bacterial detection a: Microfluidic platform for multiple detection of E. coli and Pseudomonas aeruginosa in blood[18]. b: Microfluidic chip for detecting drug resistance at single cell level[20]. c: Salmonella typhimurium microfluidic biosensor based on magnetic separation, enzyme catalysis and electrochemical impedance analysis[22]. d: One-pot digital microfluidic system using LNA/DNA molecular beacons for bacterial detection and absolute quantification[23]

References 32

[1]	Zhang DX, Bi HY, Liu BH, et al. Detection of pathogenic microorganisms by microfluidics based analytical methods[J]. Anal Chem, 2018, 90(9): 5512-5520. doi: 10.1021/acs.analchem.8b00399 pmid: 29595252
[2]	Trinh TND, Lee NY. Advances in nucleic acid amplification-based microfluidic devices for clinical microbial detection[J]. Chemosensors, 2022, 10(4): 123. doi: 10.3390/chemosensors10040123 URL
[3]	Fernandes AC, Semenova D, Panjan P, et al. Multi-function microfluidic platform for sensor integration[J]. N Biotechnol, 2018, 47: 8-17. doi: 10.1016/j.nbt.2018.03.001 URL
[4]	Ahmed I, Akram Z, Bule M, et al. Advancements and potential applications of microfluidic approaches—a review[J]. Chemosensors, 2018, 6(4): 46. doi: 10.3390/chemosensors6040046 URL
[5]	Ait M L, Mozokhina A, Tokarev A, et al. Virus replication and competition in a cell culture: application to the SARS-CoV-2 variants[J]. Appl Math Lett, 2022, 133: 108217. doi: 10.1016/j.aml.2022.108217 URL
[6]	Li X, Zhao XY, Yang WH, et al. Stretch-driven microfluidic chip for nucleic acid detection[J]. Biotechnol Bioeng, 2021, 118(9): 3559-3568. doi: 10.1002/bit.v118.9 URL
[7]	Su WD, Qiu JJ, Mei Y, et al. A microfluidic cell chip for virus isolation via rapid screening for permissive cells[J]. Virol Sin, 2022, 37(4): 547-557. doi: 10.1016/j.virs.2022.04.011 pmid: 35504535
[8]	Saraf N, Villegas M, Willenberg BJ, et al. Multiplex viral detection platform based on a aptamers-integrated microfluidic channel[J]. ACS Omega, 2019, 4(1): 2234-2240. doi: 10.1021/acsomega.8b03277 pmid: 30729227
[9]	Guan XJ, Wu F, Mao M, et al. A microfluidic platform for the ultrasensitive detection of human enterovirus 71[J]. Sens Actuat Rep, 2021, 3: 100046.
[10]	Eivazzadeh-Keihan R, Pashazadeh-Panahi P, Mahmoudi T, et al. Dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors[J]. Mikrochim Acta, 2019, 186(6): 329. doi: 10.1007/s00604-019-3420-y pmid: 31055654
[11]	Tian F, Liu C, Deng JQ, et al. A fully automated centrifugal microfluidic system for sample-to-answer viral nucleic acid testing[J]. Sci China Chem, 2020, 63(10): 1498-1506. doi: 10.1007/s11426-020-9800-6
[12]	Li ZY, Ding X, Yin K, et al. Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system[J]. Biosens Bioelectron, 2022, 199: 113865. doi: 10.1016/j.bios.2021.113865 URL
[13]	Yao YH, Zhao N, Jing WW, et al. A self-powered rapid loading microfluidic chip for vector-borne viruses detection using RT-LAMP[J]. Sens Actuat B, 2021, 333: 129521. doi: 10.1016/j.snb.2021.129521 URL
[14]	Kim S, Akarapipad P, Nguyen BT, et al. Direct capture and smartphone quantification of airborne SARS-CoV-2 on a paper microfluidic chip[J]. Biosens Bioelectron, 2022, 200: 113912. doi: 10.1016/j.bios.2021.113912 URL
[15]	Zhang Y, Hu XZ, Wang QJ. Review of microchip analytical methods for the determination of pathogenic Escherichia coli[J]. Talanta, 2021, 232: 122410. doi: 10.1016/j.talanta.2021.122410 URL
[16]	Boehm DA, Gottlieb PA, Hua SZ. On-chip microfluidic biosensor for bacterial detection and identification[J]. Sens Actuat B, 2007, 126(2): 508-514. doi: 10.1016/j.snb.2007.03.043 URL
[17]	Mairhofer J, Roppert K, Ertl P. Microfluidic systems for pathogen sensing: a review[J]. Sensors, 2009, 9(6): 4804-4823. doi: 10.3390/s90604804 pmid: 22408555
[18]	Costa SP, Caneira CRF, Chu V, et al. A microfluidic platform combined with bacteriophage receptor binding proteins for multiplex detection of Escherichia coli and Pseudomonas aeruginosa in blood[J]. Sens Actuat B, 2023, 376: 132917. doi: 10.1016/j.snb.2022.132917 URL
[19]	Kim S, Romero-Lozano A, Hwang DS, et al. A guanidinium-rich polymer as a new universal bioreceptor for multiplex detection of bacteria from environmental samples[J]. J Hazard Mater, 2021, 413: 125338. doi: 10.1016/j.jhazmat.2021.125338 URL
[20]	Song KN, Yu ZQ, Zu XY, et al. Microfluidic chip for detection of drug resistance at the single-cell level[J]. Micromachines, 2022, 14(1): 46. doi: 10.3390/mi14010046 URL
[21]	Sun DL, Fan TT, Liu F, et al. A microfluidic chemiluminescence biosensor based on multiple signal amplification for rapid and sensitive detection of E.coli O157: H7[J]. Biosens Bioelectron, 2022, 212: 114390. doi: 10.1016/j.bios.2022.114390 URL
[22]	Liu YJ, Jiang D, Wang SY, et al. A microfluidic biosensor for rapid detection of Salmonella typhimurium based on magnetic separation, enzymatic catalysis and electrochemical impedance analysis[J]. Chin Chem Lett, 2022, 33(6): 3156-3160. doi: 10.1016/j.cclet.2021.10.064 URL
[23]	Kao YT, Calabrese S, Borst N, et al. Microfluidic one-pot digital droplet FISH using LNA/DNA molecular beacons for bacteria detection and absolute quantification[J]. Biosensors, 2022, 12(4): 237. doi: 10.3390/bios12040237 URL
[24]	Cao DJ, Wu S, Xi CL, et al. Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization[J]. Commun Biol, 2021, 4(1): 1224. doi: 10.1038/s42003-021-02762-2
[25]	Zhou WT, Le J, Chen Y, et al. Recent advances in microfluidic devices for bacteria and fungus research[J]. Trac Trends Anal Chem, 2019, 112: 175-195. doi: 10.1016/j.trac.2018.12.024 URL
[26]	Soares RRG, Santos DR, Pinto IF, et al. Point-of-use ultrafast single-step detection of food contaminants: a novel microfluidic fluorescence-based immunoassay with integrated photodetection[J]. Procedia Eng, 2016, 168: 329-332. doi: 10.1016/j.proeng.2016.11.208 URL
[27]	Schell WA, Benton JL, Smith PB, et al. Evaluation of a digital microfluidic real-time PCR platform to detect DNA of Candida albicans in blood[J]. Eur J Clin Microbiol Infect Dis, 2012, 31(9): 2237-2245. doi: 10.1007/s10096-012-1561-6 URL
[28]	Wulff-Burchfield E, Schell WA, Eckhardt AE, et al. Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens[J]. Diagn Microbiol Infect Dis, 2010, 67(1): 22-29. doi: 10.1016/j.diagmicrobio.2009.12.020 URL
[29]	Wang AY, Wu ZH, Huang YH, et al. A 3D-printed microfluidic device for qPCR detection of macrolide-resistant mutations of Mycoplasma pneumoniae[J]. Biosensors, 2021, 11(11): 427. doi: 10.3390/bios11110427 URL
[30]	Dean D, Turingan RS, Thomann HU, et al. A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis[J]. PLoS One, 2012, 7(12): e51685. doi: 10.1371/journal.pone.0051685 URL
[31]	Ye X, Li Y, Wang LJ, et al. All-in-one microfluidic nucleic acid diagnosis system for multiplex detection of sexually transmitted pathogens directly from genitourinary secretions[J]. Talanta, 2021, 221: 121462. doi: 10.1016/j.talanta.2020.121462 URL
[32]	Li L, Miao BG, Li Z, et al. Sample-to-answer hepatitis B virus DNA detection from whole blood on a centrifugal microfluidic platform with double rotation axes[J]. ACS Sens, 2019, 4(10): 2738-2745. doi: 10.1021/acssensors.9b01270 URL