Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (10): 292-303.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0281

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Whole Genome Sequencing Analysis of a Phenol-degrading Strain Alcaligenes faecalis JF101

ZHANG Ao-jie1(), LI Qing-yun1,2(), SONG Wen-hong1, YAN Shao-hui1, TANG Ai-xing1,2, LIU You-yan1,2   

  1. 1. College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004
    2. Laboratory of Biological Refining in Guangxi, Nanning 530004
  • Received:2023-03-27 Online:2023-10-26 Published:2023-11-28
  • Contact: LI Qing-yun E-mail:zhangaojieao@163.com;qyli@gxu.edu.cn

Abstract:

The genus Alcaligenes shows extensive degradation capacity for aromatic compounds. In the present study, the phenol-degrading genes in Alcaligenes faecalis JF101 were analyzed and the potential functions were explored. The whole genome of strain JF101 was sequenced, assembled and annotated for analyzing the genes involved in phenol degradation. Moreover, the comparative genomics with five related strains was carried out. Results showed that the whole genome size of strain JF101 was 4 143 816 bp, and the GC content was 57.44%, with 3 804 protein-coding genes, 55 tRNAs genes, 9 rRNAs genes and 5 sRNA. There were 3 040, 2 529 and 2 415 annotated genes in COG, GO and KEGG databases, respectively. Eleven genes involving in phenol degradation were identified. Comparative genomics revealed that strain JF101 had no plasmid, and shared 2 008 common homologous gene families and 5 specific gene families with the five related strains. In addition, the putative gene Trk and Kdp involved in compatible solute transport, were found in strain JF101, and the salt-tolerant ability was confirmed by experiments. The whole-genome analysis of strain JF101 will benefit the further elucidating mechanism of phenol degradation and developing engineering applications.

Key words: Alcaligenes faecalis, phenol degradation, whole genome sequencing