Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (12): 276-286.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0710
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TU Xiao-yuan1(), CHU Lu-lu1, WANG Mian1, CHEN Bing-zhi1,2(), JIANG Yu-ji1,2()
Received:
2023-07-24
Online:
2023-12-26
Published:
2024-01-11
Contact:
CHEN Bing-zhi, JIANG Yu-ji
E-mail:a2693050400@163.com;cbz_2006@163.com;jyj1209@163.com
TU Xiao-yuan, CHU Lu-lu, WANG Mian, CHEN Bing-zhi, JIANG Yu-ji. Extraction of Polysaccharide from Hericium corallinum and Analysis on Its in vitro Antioxidant Activity[J]. Biotechnology Bulletin, 2023, 39(12): 276-286.
因素 Factor | 水平Level | |||
---|---|---|---|---|
-1 | 0 | 1 | ||
A温度 Temperature/℃ | 80 | 90 | 100 | |
B时间Time/h | 1.5 | 2.0 | 2.5 | |
C料液比 Solid-liquid ratio/(g·mL-1) | 1∶20 | 1∶30 | 1∶40 | |
D颗粒大小 Particle size/(mesh) | 60 | 80 | 100 |
Table 1 Factors and levels of Box-Behnken design
因素 Factor | 水平Level | |||
---|---|---|---|---|
-1 | 0 | 1 | ||
A温度 Temperature/℃ | 80 | 90 | 100 | |
B时间Time/h | 1.5 | 2.0 | 2.5 | |
C料液比 Solid-liquid ratio/(g·mL-1) | 1∶20 | 1∶30 | 1∶40 | |
D颗粒大小 Particle size/(mesh) | 60 | 80 | 100 |
Fig. 1 Effects of different extraction conditions on the extraction rates of polysaccharides The error line in the figure represents the standard deviation, and the lowercase letter represents a significant difference P < 0.05. The same below
试验号 Test No. | A | B | C | D | 提取率 Extraction rate Y/% |
---|---|---|---|---|---|
1 | -1 | 1 | 0 | 0 | 9.86 |
2 | 1 | 1 | 0 | 0 | 10.41 |
3 | -1 | -1 | 0 | 0 | 8.79 |
4 | 1 | -1 | 0 | 0 | 10.17 |
5 | 0 | 0 | -1 | -1 | 6.77 |
6 | 0 | 0 | 1 | -1 | 9.96 |
7 | 0 | 0 | -1 | 1 | 7.62 |
8 | 0 | 0 | 1 | 1 | 9.35 |
9 | -1 | 0 | 0 | -1 | 9.00 |
10 | 1 | 0 | 0 | -1 | 9.88 |
11 | -1 | 0 | 0 | 1 | 8.98 |
12 | 1 | 0 | 0 | 1 | 10.42 |
13 | 0 | 1 | -1 | 0 | 8.05 |
14 | 0 | -1 | -1 | 0 | 6.87 |
15 | 0 | 1 | 1 | 0 | 9.84 |
16 | 0 | -1 | 1 | 0 | 10.18 |
17 | -1 | 0 | -1 | 0 | 6.90 |
18 | 1 | 0 | -1 | 0 | 8.05 |
19 | -1 | 0 | 1 | 0 | 10.02 |
20 | 1 | 0 | 1 | 0 | 10.13 |
21 | 0 | 1 | 0 | -1 | 10.12 |
22 | 0 | -1 | 0 | -1 | 9.27 |
23 | 0 | 1 | 0 | 1 | 10.19 |
24 | 0 | -1 | 0 | 1 | 9.64 |
25 | 0 | 0 | 0 | 0 | 10.91 |
26 | 0 | 0 | 0 | 0 | 10.93 |
27 | 0 | 0 | 0 | 0 | 10.64 |
28 | 0 | 0 | 0 | 0 | 10.68 |
29 | 0 | 0 | 0 | 0 | 10.98 |
Table 2 Box-Behnken experimental design and results
试验号 Test No. | A | B | C | D | 提取率 Extraction rate Y/% |
---|---|---|---|---|---|
1 | -1 | 1 | 0 | 0 | 9.86 |
2 | 1 | 1 | 0 | 0 | 10.41 |
3 | -1 | -1 | 0 | 0 | 8.79 |
4 | 1 | -1 | 0 | 0 | 10.17 |
5 | 0 | 0 | -1 | -1 | 6.77 |
6 | 0 | 0 | 1 | -1 | 9.96 |
7 | 0 | 0 | -1 | 1 | 7.62 |
8 | 0 | 0 | 1 | 1 | 9.35 |
9 | -1 | 0 | 0 | -1 | 9.00 |
10 | 1 | 0 | 0 | -1 | 9.88 |
11 | -1 | 0 | 0 | 1 | 8.98 |
12 | 1 | 0 | 0 | 1 | 10.42 |
13 | 0 | 1 | -1 | 0 | 8.05 |
14 | 0 | -1 | -1 | 0 | 6.87 |
15 | 0 | 1 | 1 | 0 | 9.84 |
16 | 0 | -1 | 1 | 0 | 10.18 |
17 | -1 | 0 | -1 | 0 | 6.90 |
18 | 1 | 0 | -1 | 0 | 8.05 |
19 | -1 | 0 | 1 | 0 | 10.02 |
20 | 1 | 0 | 1 | 0 | 10.13 |
21 | 0 | 1 | 0 | -1 | 10.12 |
22 | 0 | -1 | 0 | -1 | 9.27 |
23 | 0 | 1 | 0 | 1 | 10.19 |
24 | 0 | -1 | 0 | 1 | 9.64 |
25 | 0 | 0 | 0 | 0 | 10.91 |
26 | 0 | 0 | 0 | 0 | 10.93 |
27 | 0 | 0 | 0 | 0 | 10.64 |
28 | 0 | 0 | 0 | 0 | 10.68 |
29 | 0 | 0 | 0 | 0 | 10.98 |
来源Source | 平方和Sum of squares | 自由度df | 均方Mean square | F值F-value | P值P-value | 显著性Significance |
---|---|---|---|---|---|---|
模型 Model | 43.150 | 14 | 3.080 | 97.78 | <0.000 1 | ** |
A | 2.530 | 1 | 2.530 | 80.26 | <0.000 1 | ** |
B | 1.050 | 1 | 1.050 | 33.32 | <0.000 1 | ** |
C | 19.300 | 1 | 19.30 | 612.40 | <0.000 1 | ** |
D | 0.120 | 1 | 0.120 | 3.81 | 0.071 3 | |
AB | 0.170 | 1 | 0.170 | 5.46 | 0.034 8 | * |
AC | 0.270 | 1 | 0.270 | 8.58 | 0.011 0 | * |
AD | 0.078 | 1 | 0.078 | 2.49 | 0.137 1 | |
BC | 0.580 | 1 | 0.580 | 18.32 | 0.000 8 | ** |
BD | 0.023 | 1 | 0.023 | 0.71 | 0.412 4 | |
CD | 0.530 | 1 | 0.530 | 16.91 | 0.001 1 | ** |
A2 | 1.780 | 1 | 1.780 | 32.73 | <0.000 1 | ** |
B2 | 1.180 | 1 | 1.180 | 31.39 | <0.000 1 | ** |
C2 | 17.290 | 1 | 17.290 | 496.43 | <0.000 1 | ** |
D2 | 3.180 | 1 | 3.180 | 117.00 | <0.000 1 | ** |
残差 Residual error | 0.440 | 14 | 0.032 | |||
失拟项 Lack of error | 0.340 | 10 | 0.034 | 1.41 | 0.395 9 | |
净误差 Pure error | 0.097 | 4 | 0.024 | |||
总误差 Cor total | 43.590 | 28 | ||||
R2=0.989 9 | R2adj=0.979 8 |
Table 3 Variance analysis table of regression model
来源Source | 平方和Sum of squares | 自由度df | 均方Mean square | F值F-value | P值P-value | 显著性Significance |
---|---|---|---|---|---|---|
模型 Model | 43.150 | 14 | 3.080 | 97.78 | <0.000 1 | ** |
A | 2.530 | 1 | 2.530 | 80.26 | <0.000 1 | ** |
B | 1.050 | 1 | 1.050 | 33.32 | <0.000 1 | ** |
C | 19.300 | 1 | 19.30 | 612.40 | <0.000 1 | ** |
D | 0.120 | 1 | 0.120 | 3.81 | 0.071 3 | |
AB | 0.170 | 1 | 0.170 | 5.46 | 0.034 8 | * |
AC | 0.270 | 1 | 0.270 | 8.58 | 0.011 0 | * |
AD | 0.078 | 1 | 0.078 | 2.49 | 0.137 1 | |
BC | 0.580 | 1 | 0.580 | 18.32 | 0.000 8 | ** |
BD | 0.023 | 1 | 0.023 | 0.71 | 0.412 4 | |
CD | 0.530 | 1 | 0.530 | 16.91 | 0.001 1 | ** |
A2 | 1.780 | 1 | 1.780 | 32.73 | <0.000 1 | ** |
B2 | 1.180 | 1 | 1.180 | 31.39 | <0.000 1 | ** |
C2 | 17.290 | 1 | 17.290 | 496.43 | <0.000 1 | ** |
D2 | 3.180 | 1 | 3.180 | 117.00 | <0.000 1 | ** |
残差 Residual error | 0.440 | 14 | 0.032 | |||
失拟项 Lack of error | 0.340 | 10 | 0.034 | 1.41 | 0.395 9 | |
净误差 Pure error | 0.097 | 4 | 0.024 | |||
总误差 Cor total | 43.590 | 28 | ||||
R2=0.989 9 | R2adj=0.979 8 |
样品Sample | 总糖Total sugar | 蛋白质Protein | 糖醛酸Uronic acid |
---|---|---|---|
HCP | 65.21±0.90 | 5.28±0.5 | 13.52±1.95 |
Table 4 Chemical composition of HCP
样品Sample | 总糖Total sugar | 蛋白质Protein | 糖醛酸Uronic acid |
---|---|---|---|
HCP | 65.21±0.90 | 5.28±0.5 | 13.52±1.95 |
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