Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (4): 221-226.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0994
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LI Yue1(), YU Wan-xian1, LI Ning2, YAO Ming-hua2, LI Feng1(), DENG Ying-tian1()
Received:
2022-08-10
Online:
2023-04-26
Published:
2023-05-16
LI Yue, YU Wan-xian, LI Ning, YAO Ming-hua, LI Feng, DENG Ying-tian. Inoculation Method for Colletotrichum in Pepper(Capsicum annuum)Seedlings[J]. Biotechnology Bulletin, 2023, 39(4): 221-226.
孢子悬 浮液浓度 Resuspend spore concentration (cells·mL-1) | 子叶期 Cotyledon | 1-2片真叶期 1st - 2nd leave | 3-4片真叶期 3rd - 4th leave | 5-10片真叶期 5th - 10thleave | 果实 Fruits | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
针刺法Needle point | 针刺法 Needle point | 注射法 Infiltration | 针刺法 Needle point | 针刺法 Needle point | 针刺法 Needle point | |||||||
感病率 Infe rate/% | 叶卷率 Curlrate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | ||
1×103 | 54.17 | 67.86 | 10.81 | 41.89 | 85.71 | 14.29 | 6.06 | 0 | 6.90 | 0 | 38.89 | |
1×104 | 100.00 | 75.00 | 60.00 | 36.00 | 100.00 | 5.00 | 61.11 | 0 | 20.83 | 0 | / | |
1×105 | 100.00 | 96.43 | 100.00 | 78.57 | 100.00 | 25.00 | 80.00 | 0 | 54.55 | 0 | 100.00 | |
1×106 | 100.00 | 100.00 | 100.00 | 80.65 | 100.00 | 41.67 | 84.38 | 0 | 72.00 | 0 | / | |
1×107 | 100.00 | 100.00 | 100.00 | 91.94 | 100.00 | 62.50 | 90.00 | 2.50 | 87.50 | 0 | 100.00 |
Table 1 Statistical analysis of plant infection and leave curl rates of pepper seedlings in different stages under different Colletotrichum spore concentration
孢子悬 浮液浓度 Resuspend spore concentration (cells·mL-1) | 子叶期 Cotyledon | 1-2片真叶期 1st - 2nd leave | 3-4片真叶期 3rd - 4th leave | 5-10片真叶期 5th - 10thleave | 果实 Fruits | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
针刺法Needle point | 针刺法 Needle point | 注射法 Infiltration | 针刺法 Needle point | 针刺法 Needle point | 针刺法 Needle point | |||||||
感病率 Infe rate/% | 叶卷率 Curlrate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | 叶卷率 Curl rate/% | 感病率 Infe rate/% | ||
1×103 | 54.17 | 67.86 | 10.81 | 41.89 | 85.71 | 14.29 | 6.06 | 0 | 6.90 | 0 | 38.89 | |
1×104 | 100.00 | 75.00 | 60.00 | 36.00 | 100.00 | 5.00 | 61.11 | 0 | 20.83 | 0 | / | |
1×105 | 100.00 | 96.43 | 100.00 | 78.57 | 100.00 | 25.00 | 80.00 | 0 | 54.55 | 0 | 100.00 | |
1×106 | 100.00 | 100.00 | 100.00 | 80.65 | 100.00 | 41.67 | 84.38 | 0 | 72.00 | 0 | / | |
1×107 | 100.00 | 100.00 | 100.00 | 91.94 | 100.00 | 62.50 | 90.00 | 2.50 | 87.50 | 0 | 100.00 |
Fig. 4 Comparing inoculated pepper seedling leaves by using infiltration and needle point method A-F: Infiltration method. G-L: Needle point method. Bar = 10 mm
Fig. 5 Colletotrichum inoculated leaves by using needle point method during different developmental stages of pepper seedlings at different Colletotrichum spore concentration
Fig. 6 Comparation of fruit needling and leaf needling methods A-D: Pepper fruits inoculated with Colletotrichum spore by using needle point method under different concentration from 1×103 to 1×107 cells/L, bars = 10 mm. E-H: Pepper seedlings inoculated with Colletotrichum spore by using needle point method under different concentration from 1×103 to 1×107 cells/L, bars = 10 mm. I: Diameter of a disease spot on inoculated pepper fruits under each concentration. J: Area of a disease spot on inoculated pepper leaves under each concentration
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