Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 216-225.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0070
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WANG Fang1(), YU Lu1, QI Ze-zheng1, ZHOU Chang-jun2, YU Ji-dong2
Received:
2024-01-16
Online:
2024-07-26
Published:
2024-07-30
Contact:
WANG Fang
E-mail:wangfangnd@hotmail.com
WANG Fang, YU Lu, QI Ze-zheng, ZHOU Chang-jun, YU Ji-dong. Screening and Biocontrol Effect of Antagonistic Bacteria against Soybean Root Rot[J]. Biotechnology Bulletin, 2024, 40(7): 216-225.
Fig. 1 Inhibitory effects of antagonistic bacteria on different Fusarium species A: Inhibitory effect of antagonistic bacteria on Fusarium strains. B: Inhibition rate colony diameter of F. graminearum. Different lowercase letters indicate significant differences at the level of 0.05, the same below
菌株 Strain | 革兰氏染色 Gram stain | 接触酶试验Contact enzyme test | 氧化酶试验 Oxidase test | 甲基红试验 Methyl red | V-P试验 V-P test | 明胶液化 Gelatin liquefaction | 吲哚试验 Indole test | 淀粉水解Starch hydrolysis test | 苯丙氨酸脱氨酶试验 Phenylalanine deaminase test |
---|---|---|---|---|---|---|---|---|---|
20-8 | + | - | + | + | - | - | - | + | - |
A54-1 | + | - | + | + | - | + | - | + | - |
A54-5 | + | - | + | + | - | - | - | + | - |
A54-6 | + | - | + | + | - | + | - | + | - |
P-7 | + | + | + | + | - | + | - | + | - |
Table 1 Physiological and biochemical characteristics of antagonistic bacteria
菌株 Strain | 革兰氏染色 Gram stain | 接触酶试验Contact enzyme test | 氧化酶试验 Oxidase test | 甲基红试验 Methyl red | V-P试验 V-P test | 明胶液化 Gelatin liquefaction | 吲哚试验 Indole test | 淀粉水解Starch hydrolysis test | 苯丙氨酸脱氨酶试验 Phenylalanine deaminase test |
---|---|---|---|---|---|---|---|---|---|
20-8 | + | - | + | + | - | - | - | + | - |
A54-1 | + | - | + | + | - | + | - | + | - |
A54-5 | + | - | + | + | - | - | - | + | - |
A54-6 | + | - | + | + | - | + | - | + | - |
P-7 | + | + | + | + | - | + | - | + | - |
菌株 Strain | 蛋白酶 Proteinase | 纤维素酶 Cellulase | 葡聚糖酶 Glucanase | 几丁质酶 Chitinase |
---|---|---|---|---|
20-8 | + | + | + | - |
A54-1 | + | + | + | - |
A54-5 | + | + | + | - |
A54-6 | + | + | + | - |
P-7 | + | + | + | - |
Table 2 Extracellular enzyme activity of antagonistic bacteria
菌株 Strain | 蛋白酶 Proteinase | 纤维素酶 Cellulase | 葡聚糖酶 Glucanase | 几丁质酶 Chitinase |
---|---|---|---|---|
20-8 | + | + | + | - |
A54-1 | + | + | + | - |
A54-5 | + | + | + | - |
A54-6 | + | + | + | - |
P-7 | + | + | + | - |
菌株 Strain | 溶磷Phosphorus dissolution | 溶钾Potassium dissolution | 固氮Nitrogen fixation | 载铁 Iron carrier |
---|---|---|---|---|
20-8 | + | + | + | + |
A54-1 | + | + | + | + |
A54-5 | + | + | + | + |
A54-6 | + | + | + | + |
P-7 | + | + | + | + |
Table 3 Growth-promoting characteristics of antagonistic bacteria
菌株 Strain | 溶磷Phosphorus dissolution | 溶钾Potassium dissolution | 固氮Nitrogen fixation | 载铁 Iron carrier |
---|---|---|---|---|
20-8 | + | + | + | + |
A54-1 | + | + | + | + |
A54-5 | + | + | + | + |
A54-6 | + | + | + | + |
P-7 | + | + | + | + |
Fig. 3 Inhibitory effects of antagonistic bacteria on the growth of F. graminearum A: Morphology of F. graminearum colony treated with fermentation broth. B: Colony diameter of F. graminearum. C: Morphology of F. graminearum colony treated with volatile metabolite. D: Colony diameter of F. graminearum
Fig. 5 Effects of strain 20-8 fermentation solution on the mycelium morphology of F. graminearum A: Mycelium untreated with fermentation solution. B-C: Mycelium treated by fermentation solution for 24 h
Fig. 6 Effects of strain 20-8 cell-free fermentation supernatant at different concentrations on spore germination of F. graminearum A: Microscopic observation of spore germination. B: Spore germination rate
处理 Treatment | 株高Plant height/cm | 根长Root length/cm | 鲜重Green weight/g | 干重Dry weight/g | 病情指数 Disease index/% | 死亡率 Mortality rate/% | 防治效果 Prevention effect/% |
---|---|---|---|---|---|---|---|
CK | 12.33±1.13ab | 15.42±2.53ab | 2.54±0.11ab | 0.27±0.03ab | |||
20-8 | 13.56±0.71a | 16.68±3.12a | 2.92±0.19a | 0.31±0.02a | |||
20-8+ F.graminearum | 11.82±0.41ab | 11.71±1.96ab | 2.10±0.13b | 0.22±0.01bc | 38.19 | 18.75 | 46.08 |
F.graminearum | 9.94±0.25b | 8.05±0.71b | 1.30±0.11c | 0.19±0.02c | 70.83 | 71.43 |
Table 4 Effects of strain 20-8 on the growth-promoting of soybean seedlings and root rot prevention
处理 Treatment | 株高Plant height/cm | 根长Root length/cm | 鲜重Green weight/g | 干重Dry weight/g | 病情指数 Disease index/% | 死亡率 Mortality rate/% | 防治效果 Prevention effect/% |
---|---|---|---|---|---|---|---|
CK | 12.33±1.13ab | 15.42±2.53ab | 2.54±0.11ab | 0.27±0.03ab | |||
20-8 | 13.56±0.71a | 16.68±3.12a | 2.92±0.19a | 0.31±0.02a | |||
20-8+ F.graminearum | 11.82±0.41ab | 11.71±1.96ab | 2.10±0.13b | 0.22±0.01bc | 38.19 | 18.75 | 46.08 |
F.graminearum | 9.94±0.25b | 8.05±0.71b | 1.30±0.11c | 0.19±0.02c | 70.83 | 71.43 |
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