Abstract:

【Objective】Eocanthecona concinna and Picromerus lewisi are important predatory natural enemy resources of agricultural and forestry pests in China. It is aimed to investigate their characteristics and differences of mitochondrial genome and explore the phylogenetic relationship of Asopinae insects. The mitochondrial genome sequence of two main insect species were determined and analyzed.【Method】The mitochondrial genome sequences of two enemies were determined and analyzed. After sequencing, the complete mitochondrial genome sequences of E. concinna and P. lewisi were obtained by splicing, correcting and annotating, and the phylogenetic trees of Asopinae insects were constructed based on the protein nucleotide and amino acid sequences using the maximum likelihood method.【Result】The complete mitochondrial genome sequences of E. concinna and P. lewisi were 18 740 bp（GenBank accession No. OR979468）and 17 181 bp（GenBank accession No. OR972558）in size, respectively. Including 13 protein-coding genes（PCGs）, two ribosomal RNA genes（rRNAs）, 22 transfer RNA genes（tRNAs）, and a control region. The same gene arrangement of the two enemies is consistent with the typical Pentatomidae insects. The control regions of the mitochondrial genomes of E. concinna and P. lewisi were located between rrnS and trnI, with lengths of 3 862 and 2 438 bp, respectively. The structures were different, and both contained random replication regions of different lengths. By comparing the similarity of the full sequence and PCGs of the mitochondrial genomes with other Asopinae insects, the results showed that the similarity between the same genus was high, and PCGs similarity between different geographical populations of P. lewisi was more than 98%. Phylogenetic analysis showed that the Picromerus species were clustered together and E. concinna, E. furcellata, E. thomsoni（GenBank accession No. OP920755）were clustered into one branch. The two genera, Eocanthecon and Picromerus, have close relationship to each other, and far relationships between Cazira and Eocanthecon or Picromerus.【Conclusion】The mitochondrial genome characteristics of E. concinna and P. lewisi have been clarified in this study, and the phylogenetic relationship of Asopinae bugs has been constructed, which was consistent with the results of traditional taxonomic identification.

Key words: Eocanthecona concinna, Picromerus lewisi, mitochondrial genome, Asopinae, phylogeny