Screening of Diesel-degrading Strains and Their Degrading Characteristics

Abstract

Abstract: Ten diesel-degrading strains were isolated from oil-contaminated samples by gradient enrichment culture and dilution coating. Strain YR2 with highest diesel degradation ability was chosen for further study. The degradation rate of strain YR2 was 92.8% when it was inoculated in mineral medium with diesel concentration of 1 g/100 mL for 7 days. The degradation rate was respectively 60.8%, 53.5% and 41.0% when the diesel concentration was 2 g/100 mL, 4 g/100 mL and 5 g/100 mL. Based on morphological, physiological-biochemical features and 16S rDNA sequences comparison, strain YR2 was identified as Pseudomonas aeruginosa. Strain YR2 has the excellent cell surface hydrophobicity, emulsifying ability and oil displacement ability. Strain YR2 can produce glycolipid biosurfactant. Strain YR2 with efficient diesel degradation ability has the potential application in the microbial remediation of diesel pollution.

Key words: Diesel, Pseudomonas sp., Glycolipid, Cell surface hydrophobicity, Microbial degradation

Yue Guilong, Chen Liang, Liu Na. Screening of Diesel-degrading Strains and Their Degrading Characteristics[J]. Biotechnology Bulletin, 2014, 0(1): 161-165.

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