Cloning and Bioinformatics Analysis of TLR3 Gene in Maiwa Yak

Abstract

Abstract: The coding sequence of Maiwa yak（Bos grunnien）TLR3 was cloned in this research. Some characteristics of the TLR3 gene and encoded protein sequences were predicted and analyzed by the methods of bioinformatics in the following aspects, including the general physical and chemical properties, secondary structure and protein domain. Results showed that the coding region sequence of Maiwa yak TLR3 contains a complete open reading frame（2 715 bp）encoding 904 amino acids. The secondary structures of the protein are mainly composed of a-helix and random coli. The phylogenetic tree shows that the gene of Maiwa yak was so close to cattle, sheep and horse. Sequence alignment of TLR3 gene also showed highly homology in yak with other species.

Key words: Maiwa yak, TLR3 gene, Cloning, Bioinformatic analysis

Chen Yabing,Lan Daoliang,Lin Baoshan,Huang Cai,Huang Yong, Li Jian. Cloning and Bioinformatics Analysis of TLR3 Gene in Maiwa Yak[J]. Biotechnology Bulletin, 2014, 0(9): 184-188.

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