On the Involvement of CKS1B in Gonad Development of Mud Crab Scylla paramamosain

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.030

Abstract

Abstract: CDC28 protein kinase regulatory subunit 1B（CKS1B）is a member of the highly conserved cyclin kinase subunit 1（CKS1）protein family,and involves in cell division of eukaryotic organisms as a regulatory subunit of cyclin-dependent kinases. Therefore,it is of significance to study the function of CKS1 family genes involved in the molecular mechanism of regulating crustacean's gonad development. In the present study,the fragment of CKS1B gene was selected from the gonad EST library of S. paramamosain,and the full-length cDNA sequence of Sp-CKS1B was cloned,further the expressions of Sp-CKS1B in different tissues and different development stages of gonad were examined using qRT-PCR. As results,the full length cDNA of Sp-CKS1B was 722 bp,including a 5'untranslated region（5' UTR）of 82 bp,a 3'UTR of 379 bp,and an open reading frame of 261 bp encoding a protein of 86 amino acid. The protein contained a typical CKS conservative sequence belonging to the CKS family. Its expression level in the ovary of O5 stage female crabs was the highest,and presented extremely significant difference from other tissues（P<0.01）. Meanwhile,the expression level of Sp-CKS1B gene in T1 was the highest among the other stages of gonad development,followed by O5 stage,both of them were significantly higher than O1-O4 stage of female with significant difference（P<0.05）. Its lowest expression level was in the T3 stage,which was significantly lower than in T1,T2,O4,and O5 stages（P<0.05）. These results suggest thatSp-CKS1B may play a critical role in the gonad development of the mud crab.

Key words: CKS1B, Scylla paramamosain, gene cloning, tissue expression, gonad development

HAN Kun-huang, DAI Yan-bin, ZOU Zhi-hua, ZHANG Zi-ping, WANG Yi-lei. On the Involvement of CKS1B in Gonad Development of Mud Crab Scylla paramamosain[J]. Biotechnology Bulletin, 2016, 32(11): 188-193.

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