Research Progress on Cysteine Participation in Heavy Metal Resistance in Organism

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.004

Abstract

Abstract: Cysteine（Cys）,the final product of the sulfate assimilation pathway,widely exists in the organism. The oxidation state sulfur is absorbed and restored,and then integrated into the molecules skeletal structure of cysteine by organisms,participating in the other metabolic pathways of organisms. Because the structure of Cys contains thiol,which makes it being able to specifically bind with heavy metal ion,thus involving in the heavy metal resistance of organism,directly or indirectly. Considering a series of physiological and biochemical process in biological cells are orderly conducted under the heterogeneous condition,the investigation of Cys and the structure characteristics of Cys-M（M represents metal ion）complexes has significant reference value for studying Cys and the physiological behavior of Cys-M at the molecular level. In recent years,the application of new technique in the biological science,for example atomic force microscope（AFM）,makes this research feasible. In this paper,the basic characteristics and the biosynthetic pathway of Cys are introduced,concurrently,the research progress of Cys involved in heavy metal resistance are summarized,which aims to provide certain scientific theoretical basis for studying the detoxification mechanism of Cys in heavy metal resistance of organism.

Key words: cysteine, biosynthetic pathway, heavy metal resistance

ZHANG Li, SUN Dui, WANG Xiao, ZHENG Chun-li. Research Progress on Cysteine Participation in Heavy Metal Resistance in Organism[J]. Biotechnology Bulletin, 2017, 33(5): 26-33.

References

[1] 孙雪梅. 植物的硫同化及其相关酶活性在镉胁迫下的调节[J]. 植物生理与分子生物学学报, 2006, 32（1）：9-16.
[2] Harada E, Yamaguchi Y, Koizumi N, et al. Cadmium stress induces production of thiol compounds and transcripts for enzymes involved in sulfur assimilation pathways in Arabidopsis[J]. Journal of Plant Physiology, 2002, 159（159）：445-448.
[3] Ilyas S, Rehman A. Oxidative stress, glutathione level and antioxidant response to heavy metals in multi-resistant pathogen, Candida tropicalis[J]. Environmental Monitoring & Assessment, 2015, 187（1）：4115-4122.
[4] 张贵珠, 王月梅, 赵鹏, 等. 以钴离子为探针测定半胱氨酸的研究[J]. 稀有金属, 1998, 22（1）：55-59.
[5] Yang NJ, Wang XX, Wan QJ. Electrochemical reduction of Zn（II）ions on L-cysteine coated gold electrodes[J]. Electrochimica Acta, 2006, 51（10）：2050-2056.
[6] 刘文涵, 单胜艳, 张丹, 等. 原子吸收硫化锌法间接测定半胱氨酸络合反应的机理研究[J]. 光谱学与光谱分析, 2005, 25（10）：1717-1719.
[7] Ghiamati E, Boroujerdi R. Cd-Cysteine nanorods as a fluorescence sensor for determination of Fe（III）in real samples[J]. Journal of Fluorescence, 2015, 26（1）：1-13.
[8] 段静静. Pseudomonas sp. QR-101生物合成L-半胱氨酸的系统生物学及相关转化途径研究[D]. 天津：南开大学, 2012.
[9] Guédon E, Martin-Verstraete I. Cysteine metabolism and its regulation in bacteria[J]. Microbiol Monographs, 2007, 5：195-218.
[10] Bick JA, Dennis JJ, Zylstra GJ, et al. Identification of a new class of 5’-adenylysulfate（APS）reductasefrom sulfate-assimilating bacteria[J]. Journal of Bactertiology, 2000, 182（1）：135-142.
[11] Brzywczy J, Sienko M, Kucharska A, et al. Sulphur amino acid synthesis in Schizosaccharomyces pombe represents a specific variant of sulphur metabolism in fungi[J]. Yeast, 2002, 19：29-35.
[12] 宋超, 郑春丽, 王建英. 微生物硫酸盐的同化途径及其与重金属抗性的关系[J]. 安徽农业科学, 2012, 40（11）：6368-6370, 6400.
[13] Ono B, Hazu T, Yoshida S, et al. Cysteine biosynthesis in Saccharomyces cerevisiae：a new outlook on pathway andregulation[J]. Yeast, 1999, 15：1365-1375.
[14] Koprivova A, Meyer AJ, Schween G, et al. Functional knockout of theadenosine 5'-phosphosulfate reductase gene in Physcomitrella patens revives an old route of sulfate assimilation[J]. Journal of Biological Chemistry, 2002, 277（35）：32195-32201.
[15] 钱林, 郑春丽, 柳建设. 嗜酸氧化亚铁硫杆菌ATP硫酸化酶的表达、纯化及性质鉴定[J]. 生物技术通报, 2012（6）：136-140.
[16] Zheng CL, Zhang YY, Liu YD, et al. Characterization and reconstitute of a[Fe4S4]adenosine 5'-phosphosulfate reductase from Acidithiobacillus ferrooxidans[J]. Current Microbiology, 2009, 58（6）：586-592.
[17] Zheng CL, Nie L, Qian L, et al. K30, H150, and H168 are essential residues for coordinating pyridoxal 5’-phosphate of O-Acetylserine sulfhydrylase from Acidithiobacillus ferrooxidans[J]. Current Microbiology, 2010, 60：461-465.
[18] 郑春丽, 李艳君, 钱林, 等. 嗜酸氧化亚铁硫杆菌半胱氨酸合成酶的表达、纯化及其性质鉴定[J]. 生物技术通报, 2011（3）：180-184.
[19] Zheng CL, Chen MJ, Tao ZL, et al. Differential expression of sulfur assimilation pathway genes in Acidithiobacillus ferrooxidans under Cd 2+ stress：evidence from transcriptional, enzymatic, and metabolic profiles[J]. Extremophiles, 2015, 19：429-436.
[20] 郑春丽, 王丹, 张礼, 等. 嗜酸氧化亚铁硫杆菌硫酸盐同化相关基因的鉴定与分析[J]. 生物技术通报, 2016, 32（2）：131-139.
[21] Thomas D, Surdin-Kerjan Y. Metabolism of sulphur amino acids in Saccharomyces cerevisiae[J]. Microbiology & Molecular Biology Reviews Mmbr, 1997, 61：503-532.
[22] 唐杰. 基于半耽氨酸与重金属离子相互作用的分析应用研究[D]. 重庆：西南大学, 2011.
[23] Yang W, Gooding JJ, Hibbert DB. Characterisation of gold eleetrodes modified with self-assembled monolayers of L-cysteine for the adsorptive stripping analysis of Copper[J]. Journal of Electroanalytical Chemistry, 2001, 516（1-2）：10-16.
[24] Prudent M, Girault HH. The role of Copper in cysteine oxidation：study of intra- and inter-molecular reactions in mass spectrometry[J]. Metallomics Integrated Biometal Science, 2009, 1（2）：157-165.
[25] 赵楠, 林璨瑜, 王淑芳, 等. 过表达B基因的转基因微型番茄的获得[J]. 南开大学学报, 2006, 39（4）：103-107.
[26] 梁智万, 林秋红, 肖启华, 等. 口服谷胱甘肽（GSH）治疗慢性铅中毒临床疗效观察[J]. 中国工业医学杂志, 1997, 10（6）：352-353.
[27] Prashant M, Nisha KR, Sudesh KY. Cadmium induced oxidative stress influence on glutathione metabolic genes of Camellia sinensis（L.）O. Kuntze[J]. Environmental Toxicology, 2007, 22（4）：368-374.
[28] 刘慧, 王晓蓉, 王为本, 等. 不同形态锌离子对鲫鱼谷胱甘肽系统的影响[J]. 中国环境学报, 2005, 25（2）：169-173.
[29] 孙琴, 王超. 土壤外源Cd和Pb复合污染对小麦（Tritioum asetivum L.）根系植物络合素和谷胱甘肽的影响[J]. 生态环境, 2008, 17（5）：1833-1838.
[30] Rehman A, Anjum MS. Multiple metal tolerance and biosorption of cadmium by Candida tropicalis isolated from industrial effluents：glutathione as detoxifying agent[J]. Environmental Monitoring & Assessment, 2011, 174（1-4）：585-595.
[31] Domínguez-Solís JR, López-Martín MC, Ager FJ, et al. Increased cysteine availability is essential for cadmium tolerance and accumulation in Arabidopsis thaliana[J]. Plant Biotechnology Journal, 2004, 2（6）：469-476.
[32] Noji M, Saito M, Nakamura M, et al. Cysteine synthase overexpression in tobacco confers tolerance to sulfur-containing environment pollutants[J]. Plant Physiol, 2001, 126（3）：973-980.
[33] Dominguez-Solis JR, Gutierrez-Alcala G, Romero LC, et al. The cytosolic O-acetylserine（thiol）lyase gene is regulated by heavy metals and can function in cadmium tolerance[J]. Journal of Biological Chemistry, 2001, 276（12）：9297-9302.
[34] Kawashima CG, Noji M, Nakamura M, et al. Heavy metal tolerance of transgenic tobacco plants over-expressing cysteine synthase[J]. Biotechnology Letters, 2004, 26（2）：153-157.
[35] Romeroy-Isart N, Vasak M. Advances in the structure and chemistry of metallothioneins[J]. Journal of Inorganic Biochemistry, 2002, 88（3-4）：388-396.
[36] 赵之伟, 曹冠华, 李涛. 金属硫蛋白的研究进展[J]. 云南大学学报：自然科学版, 2013, 35（3）：390-398.
[37] 张艳, 杨传平. 金属硫蛋白的研究进展[J]. 分子植物育种, 2006, 4（3）：73-78.
[38] Haq F, Mahoney M, Koropatnick J. Signaling events for metallothionein induction[J]. Mutation Research/fundamental & Molecular Mechanisms of Mutagenesis, 2003, 533（1-2）：211-226.
[39] 田晓丽, 郭军华. 金属硫蛋白的研究进展[J]. 国外医学药学分册, 2005, 32（2）：119-124.
[40] Li LS, Meng YP, Cao QF, et al. Type 1 metallothionein（ZjMT）is responsible for heavy metal tolerance in Ziziphus jujube[J]. Biochemistry, 2016, 81（6）：565-573.
[41] Vallee BL. Introduction to metallothionein[J]. Methods Enzymol, 1991, 205（1）：3-7.
[42] Cobbett CP. Goldsbrough. Phytochelatins and metallothioneins：roles in heavy metal detoxification and homeostasis[J]. Plant Biology, 2002, 53（53）：159-182.
[43] Coyle P, Philcox JC, Carey LC, et al. Metallothionein：the multipurpose protein[J]. Cellular & Molecular Life Sciences Cmls, 2002, 59（4）：627-647.
[44] Kassim R, Ramseyer C, Enescu M. Oxidation reactivity of zinc-cysteine clusters in metallothionein[J]. Journal of Biological Inorganic Chemistry, 2013, 18（3）：333-342.
[45] Lavradas RT, Hauser-Davis RA, Lavandier RC, et al. Metal, metallothionein and glutathione levels in blue crab（Callinectes sp. ）specimens from southeastern Brazil[J]. Ecotoxicology & Environmental Safety, 2014, 107（9）：55-60.
[46] Wang C, Zhang F, Cao W, et al. The identification of metallothionein in rare minnow（Gobiocypris rarus）and its expression following heavy metal exposure[J]. Environmental Toxicology & Pharmacology, 2014, 37（3）：1283-1291.
[47] Han YL, Zhang S, Liu GD, et al. Cloning, characterization and cadmium inducibility of metallothionein in the testes of the mudskipper Boleophthalmus pectinirostris[J]. Ecotoxicology & Environmental Safety, 2015, 119：1-8.
[48] Santovito G, Boldrin F, Irato, P. Metal and metallothionein distribution in different tissues of the mediterranean clam Venerupis philippinarum during copper treatment and detoxification[J]. Comparative Biochemistry & Physiology Part C Toxicology & Pharmacology, 2015, 174-175：46-53.
[49] Murphy A, Taiz L. Comparison of metallothionein gene expression and nonprotein thiols in ten Arabidopsis ecotypes[J]. Plant Physiology, 1995, 109（3）：945-954.
[50] Dundar E, Sonmez GD, Unver T. Isolation, molecular characterization and functional analysis of OeMT2, an olive metallothionein with a bioremediation potential[J]. Molecular Genetics and Genomics, 2015, 290（1）：187-199.
[51] Tomas M, Pagani MA, Andreo CS, et al. Sunflower metallothionein family characterization. Study of the Zn（II）- and Cd（II）-binding abilities of the HaMT1 and HaMT2 isoforms[J]. Journal of Inorganic Biochemistry, 2015, 148：35-48.
[52] Klaassen CD, Liu J, Choudhuri S. Metallothionein：an intracellular protein to protect against cadmium toxicity[J]. Pharmacology and Toxicology, 1999, 39（39）：267-294.
[53] 卢永科, 川岛明, 堀井郁夫, 等. 顺铂对大鼠肝细胞毒性及谷胱甘肽的保护作用[J]. 中国公共卫生, 2004, 20（4）：440-441.
[54] Magda M, Helmut S. Cytotoxicity of metals in isolated fish cells：Importance of the cellular glutathione status[J]. Comparative Biochemistry and Physiology PartA, 1998, 120（1）：83-88.
[55] 阮湘元, 彭敏, 徐经伟, 等. 谷胱甘肽在汞表面吸附与自组装行为的原子力显微镜研究[J]. 分析化学研究简报, 2005, 32（11）：1587-1589.
[56] 彭敏. 谷胱甘肽对Cd 的解毒机理研究[J]. 东莞理工学院学报, 2014, 21（5）：69-73.
[57] 刘建华, 李燕, 王海军. 量子化学研究Cd 2+ 、Hg 2+ 、Pb 2+ 与谷肤甘肤相互作用[J]. 计算机与应用化学, 2013, 30（2）：141-146.
[58] Vicky M, Farideh J. Mercury（II）complex formation with glutathione in alkaline aqueous solution[J]. Journal of Biological Inorganic Chemistry, 2008, 13（4）：541 -553.
[59] Aviva L, Zhang LB, Peter LA. Structure and reactivity of a chromium（V）glutathione complex[J]. Inorganic Chemistry, 2003, 42（3）：767-784.
[60] Cheng F, Zhou XY. Electrochemical studies of glutathione monolayer assembled on a polycrystalline gold electrode[J]. Wuhan University Journal of Natural Sciences, 2002, 7（1）：102 -106.
[61] Cheng F, Zhou XY. Voltammetry and EQCM investigation of glutathione monolayer and its complex ation with Cu 2+ [J]. Electroanalysis, 2003, 15（20）：1632 -1638.
[62] Cobbett CS. Phytochelatin biosynthesis and function in heavy-metal detoxification[J]. Current Opinion in Plant Biology, 2000, 3（3）：211-216.
[63] Cobbet CS. Phytochelatin and their roles in heavy metal detoxification[J]. Plant Physiology, 2000, 123（3）：825-832.