Effects of Major Enzymes in the Biosynthetic Pathway of Vitamin K2 on MK-7 Production

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.032

Abstract

Abstract: This paper focused on understanding the effects of different enzyme genes on MK-7 production by cloning and expressing the genes of 6 enzymes in the biosynthetic pathway of vitamin K₂ with strain Y-2. Firstly,the recombinant plasmids with single gene of the 6 genes were constructed according to the description of Guide to Molecular Cloning Experiment. Subsequently,the recombinant plasmids were successfully transformed into the strain Y-2 via the improved “spizizen” transformation method. Results showed that respective overexpression of the 6 genes involved in vitamin K₂ biosynthetic pathway all was beneficial to raise MK-7 production. It should be noted that overexpression of gene hep S had the greatest effects on MK-7 production among these 6 genes,which resulted in an increase by 38% in shaking group and by 48% in static group. However,the effects of other genes on MK-7 production varied depending on the culture mode.

Key words: Bacillus amyloliquefaciens, menaquinone-7, biosynthetic pathway, catalyzing enzyme

XU Jian-zhong, WANG Ying-yu, YAN Wei-liu, ZHANG Wei-guo. Effects of Major Enzymes in the Biosynthetic Pathway of Vitamin K₂on MK-7 Production[J]. Biotechnology Bulletin, 2016, 32(11): 248-254.

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Effects of Major Enzymes in the Biosynthetic Pathway of Vitamin K₂on MK-7 Production

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