Effects of Nitric Oxide on Oxidative Damage Metabolism in Wheat Seedling Under Cadmium Stress

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.015

Abstract

Abstract: This study is to further study the response mechanism of nitric oxide gas signal on oxidative damage of wheat seedlings under cadmium（Cd）stress. Through nutrient solution cultivation,Triticum aestivum ‘Jinmai 8’ was selected as the plant material,and nitric oxide（NO）-donor sodium nitroprusside（SNP）,NO-scavenger haemoglobin（Hb）,and sodium ferrocyanide（SNP analogue）were used to investigate the effects of NO on oxidative damage metabolism in seedlings under Cd stress. The results showed that SNP alleviated the growth inhibition in wheat seedlings,significantly increased the contents of soluble protein,chlorophyll and glutathion,decreased the contents of malondiadehyde（MDA）and hydrogen peroxide（H₂O₂）,as well as the production of O₂·^-,and the accumulations of soluble sugar and proline. However,the NO-scavenger hemoglobin treatment increased the toxicity of Cd stress to wheat seedlings,and the SNP analogue treatment presented no effect. Meanwhile,SNP decreased the activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione reductase（GR）and ascorbate peroxidase（APX）,which eliminated the oxidative damage under Cd stress. Conclusively,NO alleviates Cd-damage by regulating the antioxidase system.

Key words: wheat seedlings, cadmium stress, nitric oxide, oxidative damage

MA Xiao-li, JI Rui-ping, TIAN Bao-hua, HUO Jian-xin. Effects of Nitric Oxide on Oxidative Damage Metabolism in Wheat Seedling Under Cadmium Stress[J]. Biotechnology Bulletin, 2017, 33(5): 102-107.

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