Application Progress on Aptasensors in Detection of Food-born Pathogenic Bacteria

doi:10.13560/j.cnki.biotech.bull.1985.2018-0608

Abstract

Abstract: Nowadays,food security is the focus of public,and food-born pathogenic bacteria are one of the main causes of food safety events. Therefore,it is necessary to develop a rapid,sensitive and Low-cost method for detection and supervision of food-borne pathogenic bacteria. Aptamers are DNA/RNA oligonuleotide binding targets with high affinity and high specificity,which are obtained from a Library of single-stand random oligonuleotide in vitro by cell-SELEX. Due to the complex surface structures of food-borne pathogenic bacteria,SELEX-based technologies for aptamer selection have been being improved constantly. This review introduces some current modified-SELEX techniques,compares 10 characterization methods for aptamer binding affinity,and summarizes important aptamers of several common food-borne pathogenic bacteria,including DNA aptamer,RNA aptamer and split aptamer. Comparing with antibodies,aptamers possess numerous advantages. Thus,aptasensors,used for the detection of food-born pathogenic bacteria,have been rapidly developed in recent years,of which the electrochemical sensors and optical sensors are the most widely applied. At Last,this review focuses on the Latest progresses on the electrochemical aptasensors and optical aptasensors for food-borne pathogenic bacteria and proposes the development trend of aptasensors. This pioneers a new field for microbial detection techniques and further ensures food safety and public’s health.

Key words: food-born pathogenic bacteria, aptamer, aptasensor, SELEX

LI Xue-tong, LIN Ying, ZHANG Yuan, LI Ying, LÜ Shu-xia, XU Wen-tao. Application Progress on Aptasensors in Detection of Food-born Pathogenic Bacteria[J]. Biotechnology Bulletin, 2019, 35(4): 125-138.

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