Cloning of σ-GST Gene in Anodonta woodiana and Its Expression Analysis Under Pentachlorophenol Stress

doi:10.13560/j.cnki.biotech.bull.1985.2018-0871

Abstract

Abstract: Glutathione S-transferases（GSTs）play a prominent role in protecting cells against oxidative stress. Our previous studies reveal that the pentachlorophenol（PCP）causes significant oxidative stress and acute toxic effects on Anodonta woodiana,but its chronic toxicity remains unclear. In order to investigate the chronic effect of PCP,A. woodiana were randomly grouped into PCP-treated group with 13.9 μg/L concentrations of PCP and control group with the same volume of dimethyl sulfoxide. Meanwhile,one GST sequences were cloned from A. woodiana and named as σ-GST,and the effect of PCP on the σ-GST was analyzed. The full-length cDNA of σ-GST consisted of 132 bp 5' untranslated region（UTR）,80 bp 3' UTR and 609 bp open reading frame of an encoded polypeptide by 203 amino acids. σ-GST had the tag sequence of GST and conserved amino acids,and had high homology with σ-like GST sequence of other species. The σ-GST was widely expressed in different tissues such as foot,mantle,adductor muscle,heart,hepatopancreas,hemocytes and gill. PCP treatment resulted in a significant increase of σ-GST expression in the hepatopancreas,gill and hemocytes. In the hepatopancreas,σ-GST mRNA level of PCP-treated group increased more than 28.73% at day 1,then 70.37%（P<0.05）at day 3,reached 6.64 times（P<0.01）at day 15 in contrast to the control group. In the gill,σ-GST mRNA expression showed a up-regulation by 1.14 times（P<0.05）in the PCP-treated group during whole experiment,compared with that of control group. These results indicate that up-regulation of σ-GST expression in A. woodiana is conducive to its resistance to the stress from PCP treatment during experiment.

Key words: Anodonta woodiana, σtype glutathione S-transferases, gene cloning, stress expression

XIA Xi-chao, LIU Qing-chun, HUA Chun-xiu, ZHANG Ke, LI Bing-jie, SONG Guo-ying, XUE Shi-peng, YU Rui-xue, WANG Zhong-xiao, ZHANG Qing-yuan, LIU Li. Cloning of σ-GST Gene in Anodonta woodiana and Its Expression Analysis Under Pentachlorophenol Stress[J]. Biotechnology Bulletin, 2019, 35(5): 93-101.

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