Abstract: This work aims to study the changes of gene expression profiles of Lycium ruthenicum Murr. under salt stress with different concentrations,and to provide a basis for further studying the molecular mechanism of salt resistance of L. ruthenicum. First,the roots and leaves of tissue culture seedlings of L ruthenicum stressed by NaCl at the concentrations of 0（CK）,50 and 250 mmol/L were sampled at stress duration of 0,1 and 12 h,respectively. Then,the sequence analysis was conducted using transcriptome sequencing technique（RNA-Seq）. The results showed that a total of 222.49 Gb raw data were generated by transcriptome sequencing,from which 86 037 Unigenes were obtained. The 46 594 Unigenes,accounting for 54.76% of the total Unigenes,were annotated in the 7 functional databases（GO,KEGG,KOG,NR,Pfam,Swiss-Prot and egg NOG）,while the rest 38 929 Unigenes were not annotated in the known genes databases. These Unigenes were classified into 51 GO categories and 211 metabolic pathways using GO classification and KEGG Pathway cluster analysis. Analysis of differentially expressed genes revealed that the up-regulated genes and down-regulated genes in the leaves and roots of L. ruthenicum increased with the NaCl concentration as well as treatment time increasing. The up-regulated genes（7 514）in leaves were less than down-regulated（9 032）genes,while the up-regulated genes（12 347）in roots were more than down-regulated genes（11 559）. Total 28 325 SSR（Simple Sequence Repeats）loci were discovered in L. ruthenicum under salt stress transcriptome,most of which were single nucleotide SSR and accounted for 70.47% of the total. Comprehensive analysis showed that the responses of L. ruthenicum to salt stress involved many genes and were regulated by several collaborative biological processes. The changes of gene expression level might be the major approach in gene regulation.

Key words: Lycium ruthenicum Murr., salt stress, transcriptome sequencing, function annotation