Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (12): 12-20.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0422
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ZHAI Nan-xin1(), CHI Hui1, XIA Yue-lin1, LIU Cai-yue2, PEI Xin-wu2(), YUAN Qian-hua1()
Received:
2020-04-14
Online:
2020-12-26
Published:
2020-12-22
Contact:
PEI Xin-wu,YUAN Qian-hua
E-mail:1045292080@qq.com;peixinwu@caas.cn;qhyuan@163.com
ZHAI Nan-xin, CHI Hui, XIA Yue-lin, LIU Cai-yue, PEI Xin-wu, YUAN Qian-hua. Transcriptome Analysis of Drought-resistant Genes in Hainan Shanlan Upland Rice[J]. Biotechnology Bulletin, 2020, 36(12): 12-20.
Gene_ID /Name | Forward primer(5'-3') | Reverse primer(5'-3') |
---|---|---|
OsLEA3-2 | CCAAGAACAAGCTGGGCGAG | CTTGAACTCCGTCGCCTTCT |
RAB21 | GCTCCAGCTCAAGCTCGTC | CATGGCATGCTGCTGCTC |
Os09g0351700 | TCTCGAACAGCAACCTGCAT | GGATGGAATTGTCCCGCTCA |
Os07g0658600 | GGGTTCGACACGTGCTACA | GGTGTGGATCACCACGTTCT |
Os02g0693700 | GTTCTACGAGCCTCTCACGG | CTGGTTGACGAGGCCAATCT |
Os11g0115100 | TCAGGAACCTCAACATGGGC | CTTGGAGCAGTCGACGGAG |
Os07g0251900 | CTTCGCTCGGGAACCTAACA | TGAGGTAGAGTGACCGGAGG |
Os01g0910800 | TGGCGCAGTACTACTCGGA | GCCGGTGCAGGAGTCGTA |
OsActin | TGGCATCTCTCAGCACATTCC | TGCACAATGGATGGGCCAGA |
Gene_ID /Name | Forward primer(5'-3') | Reverse primer(5'-3') |
---|---|---|
OsLEA3-2 | CCAAGAACAAGCTGGGCGAG | CTTGAACTCCGTCGCCTTCT |
RAB21 | GCTCCAGCTCAAGCTCGTC | CATGGCATGCTGCTGCTC |
Os09g0351700 | TCTCGAACAGCAACCTGCAT | GGATGGAATTGTCCCGCTCA |
Os07g0658600 | GGGTTCGACACGTGCTACA | GGTGTGGATCACCACGTTCT |
Os02g0693700 | GTTCTACGAGCCTCTCACGG | CTGGTTGACGAGGCCAATCT |
Os11g0115100 | TCAGGAACCTCAACATGGGC | CTTGGAGCAGTCGACGGAG |
Os07g0251900 | CTTCGCTCGGGAACCTAACA | TGAGGTAGAGTGACCGGAGG |
Os01g0910800 | TGGCGCAGTACTACTCGGA | GCCGGTGCAGGAGTCGTA |
OsActin | TGGCATCTCTCAGCACATTCC | TGCACAATGGATGGGCCAGA |
Sample name | Total reads | Total mapped | Multiple mapped | Uniquely mapped | Non-splice reads | Splice reads |
---|---|---|---|---|---|---|
No-drought | 57 202 689 | 53 682 100 93.77% | 1 407 400 2.45% | 52 274 700 91.32% | 33 494 592 58.51% | 18 780 108 32.81% |
Drought | 51 734 760 | 48 915 870 94.56% | 1 027 373 1.99% | 47 888 497 92.57% | 30 689 990 59.31% | 17 198 507 33.27% |
Sample name | Total reads | Total mapped | Multiple mapped | Uniquely mapped | Non-splice reads | Splice reads |
---|---|---|---|---|---|---|
No-drought | 57 202 689 | 53 682 100 93.77% | 1 407 400 2.45% | 52 274 700 91.32% | 33 494 592 58.51% | 18 780 108 32.81% |
Drought | 51 734 760 | 48 915 870 94.56% | 1 027 373 1.99% | 47 888 497 92.57% | 30 689 990 59.31% | 17 198 507 33.27% |
Baishanuo upland rice | Up | Down | Total |
---|---|---|---|
Known genes | 1 586 | 1 064 | 2 650 |
Unknown Gene | 83 | 58 | 141 |
Total | 1 669 | 1 122 | 2 791 |
Baishanuo upland rice | Up | Down | Total |
---|---|---|---|
Known genes | 1 586 | 1 064 | 2 650 |
Unknown Gene | 83 | 58 | 141 |
Total | 1 669 | 1 122 | 2 791 |
TF family | Gene_ID | Discription | Readcount | ||
---|---|---|---|---|---|
CK | D | ||||
Znf(RING-finger) | Os03g0304400 | Zinc finger,RING/FYVE/PHD-type domain containing protein. | 99.68 | 260.35 | |
Znf | Os01g0785900 | Zinc finger,C2H2-type domain containing protein;Zinc finger,C2H2-type domain containing protein. | 0.00 | 48.73 | |
Znf(WRKY) | Os02g0652100 | Similar to WRKY-like DNA-binding protein. | 3.27 | 63.90 | |
Aux/IAA | Os03g0797800 | AUX/IAA protein family protein. | 0.56 | 136.83 | |
NAC | Os01g0191300 | Similar to NAC-type transcription factor;Similar to NAC-type transcription factor;Similar to NAC-type transcription factor. | 273.74 | 554.36 | |
MYB/MYC | Os04g0594100 | MYB transcription factor,Regulation of cellulose biosynthesis during secondary cell wall formation. | 11.65 | 210.07 | |
AP2/ERF | Os02g0654700 | AP2/ERF family protein,Abiotic stress response. | 1 609.36 | 693.36 | |
bZIP | Os01g0756200 | Similar to VirE2-interacting protein VIP1. | 1.69 | 107.25 | |
HD-ZIP | Os02g0649300 | HD-ZIP I protein,Transcription activator,Stress response,Panicle development . | 12.33 | 2 541.59 | |
HSF | Os05g0530400 | Heat stress transcription factor Spl7(Heat shock transcription factor)(Heat shock factor RHSF10). | 1 154.08 | 362.62 | |
DREB | Os04g0549700 | Similar to Dehydration responsive element binding protein 1F(DREB1F protein). | 9.16 | 227.34 |
TF family | Gene_ID | Discription | Readcount | ||
---|---|---|---|---|---|
CK | D | ||||
Znf(RING-finger) | Os03g0304400 | Zinc finger,RING/FYVE/PHD-type domain containing protein. | 99.68 | 260.35 | |
Znf | Os01g0785900 | Zinc finger,C2H2-type domain containing protein;Zinc finger,C2H2-type domain containing protein. | 0.00 | 48.73 | |
Znf(WRKY) | Os02g0652100 | Similar to WRKY-like DNA-binding protein. | 3.27 | 63.90 | |
Aux/IAA | Os03g0797800 | AUX/IAA protein family protein. | 0.56 | 136.83 | |
NAC | Os01g0191300 | Similar to NAC-type transcription factor;Similar to NAC-type transcription factor;Similar to NAC-type transcription factor. | 273.74 | 554.36 | |
MYB/MYC | Os04g0594100 | MYB transcription factor,Regulation of cellulose biosynthesis during secondary cell wall formation. | 11.65 | 210.07 | |
AP2/ERF | Os02g0654700 | AP2/ERF family protein,Abiotic stress response. | 1 609.36 | 693.36 | |
bZIP | Os01g0756200 | Similar to VirE2-interacting protein VIP1. | 1.69 | 107.25 | |
HD-ZIP | Os02g0649300 | HD-ZIP I protein,Transcription activator,Stress response,Panicle development . | 12.33 | 2 541.59 | |
HSF | Os05g0530400 | Heat stress transcription factor Spl7(Heat shock transcription factor)(Heat shock factor RHSF10). | 1 154.08 | 362.62 | |
DREB | Os04g0549700 | Similar to Dehydration responsive element binding protein 1F(DREB1F protein). | 9.16 | 227.34 |
TF family | Up-regulated | Down-regulated | Percentage/% |
---|---|---|---|
Znf(RING-finger) | 22 | 16 | 20.65 |
Znf | 41 | 48 | 48.37 |
Znf(WRKY) | 1 | 24 | 13.59 |
Aux/IAA | 16 | 6 | 11.96 |
NAC | 14 | 7 | 11.41 |
MYB/MYC | 17 | 1 | 9.78 |
AP2/ERF | 14 | 3 | 9.24 |
bZIP | 10 | 3 | 7.07 |
HD-ZIP | 2 | 0 | 1.09 |
HSF | 0 | 1 | 0.54 |
DREB | 1 | 0 | 0.54 |
TF family | Up-regulated | Down-regulated | Percentage/% |
---|---|---|---|
Znf(RING-finger) | 22 | 16 | 20.65 |
Znf | 41 | 48 | 48.37 |
Znf(WRKY) | 1 | 24 | 13.59 |
Aux/IAA | 16 | 6 | 11.96 |
NAC | 14 | 7 | 11.41 |
MYB/MYC | 17 | 1 | 9.78 |
AP2/ERF | 14 | 3 | 9.24 |
bZIP | 10 | 3 | 7.07 |
HD-ZIP | 2 | 0 | 1.09 |
HSF | 0 | 1 | 0.54 |
DREB | 1 | 0 | 0.54 |
Gene_ID | Discription | Readcount | ||
---|---|---|---|---|
CK | D | |||
Os09g0351700 | Protein kinase,catalytic domain domain containing protein. | 169.76 | 2.51 | |
Os11g0115100 | Similar to Lipid transfer protein. | 0.42 | 1 909.17 | |
Os07g0658600 | Similar to Nucleoid DNA-binding-like protein. | 1.06 | 510.01 | |
Os07g0251900 | Leucine-rich repeat,N-terminal domain containing protein. | 829.35 | 20.05 | |
Os02g0693700 | Multidrug resistance protein,putative,expressed | 0.84 | 320.03 | |
Os01g0910800 | Conserved hypothetical protein. | 2.58 | 3 210.34 |
Gene_ID | Discription | Readcount | ||
---|---|---|---|---|
CK | D | |||
Os09g0351700 | Protein kinase,catalytic domain domain containing protein. | 169.76 | 2.51 | |
Os11g0115100 | Similar to Lipid transfer protein. | 0.42 | 1 909.17 | |
Os07g0658600 | Similar to Nucleoid DNA-binding-like protein. | 1.06 | 510.01 | |
Os07g0251900 | Leucine-rich repeat,N-terminal domain containing protein. | 829.35 | 20.05 | |
Os02g0693700 | Multidrug resistance protein,putative,expressed | 0.84 | 320.03 | |
Os01g0910800 | Conserved hypothetical protein. | 2.58 | 3 210.34 |
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