Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (12): 12-20.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0422

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Transcriptome Analysis of Drought-resistant Genes in Hainan Shanlan Upland Rice

ZHAI Nan-xin1(), CHI Hui1, XIA Yue-lin1, LIU Cai-yue2, PEI Xin-wu2(), YUAN Qian-hua1()   

  1. 1. College of Tropical Crops,Hainan University,Haikou 570228
    2. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2020-04-14 Online:2020-12-26 Published:2020-12-22
  • Contact: PEI Xin-wu,YUAN Qian-hua E-mail:1045292080@qq.com;peixinwu@caas.cn;qhyuan@163.com

Abstract:

Shanlan upland rice is an unique local upland rice in Hainan province,which is rich in drought- resistant gene resources. In this study,a Hainan Shanlan upland rice variety,Baishanuo was used to study the changes of transcriptome level after simulated drought stress. The main results were as follows:2 791 genes were differentially expressed after simulated drought stress,and 184 transcription factors were significantly up-regulated after simulated drought stress. These transcription factors include Znf,AP2/ERF,MYB,NAC,bZIP and so on,among which the number of Znf transcription factors was the largest. Expression analysis showed that some unannotated genes,such as Os01g0910800,their expressions increased with the time of stress,and the multiple of expression was obvious. The transcriptome data and digital gene expression profiling data obtained in this study provide information for the study of drought resistance mechanism of Shanlan upland rice,and lay a foundation for further analysis of the drought resistance mechanism of Shanlan upland rice.

Key words: Shanlan upland rice, upland rice, RNA-seq, drought resistance gene