Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (5): 279-285.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0746
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SUN Bao-zhen1(), QUAN Long-ping1, KANG Hui1, YAO Yu-xin1, SHEN Tian2, CHEN Wei-ping2, DU Yuan-peng1, GAO Zhen1()
Received:
2021-06-08
Online:
2022-05-26
Published:
2022-06-10
Contact:
GAO Zhen
E-mail:q864070339@163.com;gaoz89@sdau.edu.cn
SUN Bao-zhen, QUAN Long-ping, KANG Hui, YAO Yu-xin, SHEN Tian, CHEN Wei-ping, DU Yuan-peng, GAO Zhen. Back-splicing Primers-based PCR Method for Specific Detection of circRNA[J]. Biotechnology Bulletin, 2022, 38(5): 279-285.
circRNA名称 Name of circRNA | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
circRNA_4363 | AGAGATGCAGAACAACAGAGTTATG | GCGGGATTGAATCTGTGAATC |
circRNA_6017 | CAGAAGGATACCGTGGAATCGAT | ACTCTGCTGCAGCCTGTAGCAT |
circRNA_6044 | CATGCAACAGCTTTCAAGCAA | GAGCCTTCCTCCTTTCTTCCA |
circRNA_7086 | TTTGCTGTTTCTGGATACGCTTCT | TGCAGCATCAACTCTTTCATTGTC |
Table 1 Primers for RT-PCR and qRT-PCR
circRNA名称 Name of circRNA | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
circRNA_4363 | AGAGATGCAGAACAACAGAGTTATG | GCGGGATTGAATCTGTGAATC |
circRNA_6017 | CAGAAGGATACCGTGGAATCGAT | ACTCTGCTGCAGCCTGTAGCAT |
circRNA_6044 | CATGCAACAGCTTTCAAGCAA | GAGCCTTCCTCCTTTCTTCCA |
circRNA_7086 | TTTGCTGTTTCTGGATACGCTTCT | TGCAGCATCAACTCTTTCATTGTC |
circRNA名称 Name of circRNA | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
circRNA_4363 | ACTGATGGAGTTGATGAGGAA | GGTGTCCTTCTTCGGTTAAACT |
circRNA_6017 | GCTCTGTAAACTTAACCAGGAAA | GCATGCCATCTGAGATAATCCT |
circRNA_6044 | AAGAGAGAGCCAAGAAGGAGA | CCTTCTTGCTTGAAAGCTGT |
circRNA_7086 | CGTTTTTTCTCAGAGAAGAAGG | ATGATGGAAGGATCAGTACCGT |
Table 2 Improved primers for RT-PCR and qRT-PCR
circRNA名称 Name of circRNA | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
circRNA_4363 | ACTGATGGAGTTGATGAGGAA | GGTGTCCTTCTTCGGTTAAACT |
circRNA_6017 | GCTCTGTAAACTTAACCAGGAAA | GCATGCCATCTGAGATAATCCT |
circRNA_6044 | AAGAGAGAGCCAAGAAGGAGA | CCTTCTTGCTTGAAAGCTGT |
circRNA_7086 | CGTTTTTTCTCAGAGAAGAAGG | ATGATGGAAGGATCAGTACCGT |
Fig. 1 Alternative back-splicing circularization of circRNA a : Juxtaposition relationship. b : Crossover relationship. c and d : Inclusion relatio-nship
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