Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (10): 243-252.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0373

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Revealing the Flavonoid Biosynthesis of Soybean GmERD15c under Salt Stress by Combined Analysis of Transcriptome and Metabolome

HAN Le-le1,2(), SONG Wen-di1, BIAN Jia-shen1, LI Yang1, YANG Shuang-sheng1, CHEN Zi-yi1, LI Xiao-wei1,2()   

  1. 1. College of Life Sciences, Jilin Agricultural University, Changchun 130118
    2. Technology Innovation Laboratory for Creation and High-value Utilization of Oil Germplasm Resources, Changchun 130118
  • Received:2024-04-18 Online:2024-10-26 Published:2024-11-20
  • Contact: LI Xiao-wei E-mail:1491410856@qq.com;xiaoweili1206@163.com

Abstract:

【Objective】 Soybean GmERD15c is one of the members of the ERD15 transcription factor family, and the gene expression and substance metabolism of soybean lines transgenic with GmERD15c gene under salt stress were explored, and the relationship between GmERD15c and soybean's tolerance to salt was revealed.【Method】 Using the transgenic soybean line and its recipient soybean ‘Dongnong 50'as the materials, and the roots after the salt stress treatment were to have analysis of transcriptomics and metabolomics.【Result】 There were significant changes in the metabolites of flavonoid synthesis pathway before and after salt stress, with significant variations in the content of dihydroquercetin, catechin, and fisetin after stress. And the content of quercetin was higher in the transgenic line of GmERD15c. Meanwhile, the combined analysis of transcriptomics and metabolomics revealed that the GmERD15c gene was closely related to the flavonoid biosynthesis-related enzymes.【Conclusion】 It is hypothesized that the soybean GmERD15c gene may affect the biosynthesis of flavonoids by regulating the enzymes related to flavonoid synthesis under salt stress, and flavonoids may reduce the oxidative stress caused by salt, which provides new clues for subsequently revealing the salt tolerance mechanism of the GmERD15c gene.

Key words: soybean, GmERD15, flavonoids, transcriptomics, metabolomics