Isolation and Function Study of Actinomycetes from Rhizosphere Soil of Soybean in the Black Soil Region

doi:10.13560/j.cnki.biotech.bull.1985.2024-0994

Abstract

Abstract:

Objective Soybean root rot, caused by Phytophthora sojae, is a serious soil-borne disease. The actinomycetes resources with antagonistic effect on P. sojae in the black soil region were explored. Method Actinomycete from the rhizosphere soil of healthy and diseased soybean plants were isolated by the dilution plate scribing method using four isolation media, and strains with inhibitory activity against P. sojae were screened by the plate confrontation method, then the cultivation characteristics, physiological and biochemical properties of candidate strains were measured, and molecular identification based on 16S rRNA was performed. Result The total 87 strains of actinomycetes were isolated from rhizosphere soil of healthy plants and total 43 strains from rhizosphere soil of diseased plants. Among the 130 strains, eleven strains showed obvious antifungal activity. HV-HDN-12 from the diseased root soil significantly inhibited P. sojae with an inhibition rate of 45.48%, and LSV-JDN-1 from the healthy root soil significantly inhibited P. sojae with an inhibition rate of 45.84%. HV-HDN-12 and LSV-JDN-1 have ACC deaminase activity and produce plant growth-promoting indoleacetic acid (IAA). LSV-JDN-1 produced siderophore. HVNDN-12 showed broad-spectrum antifungal activity and was identified as Streptomyces albidoflavus based on its morphological, physiological and biochemical results and 16S rRNA sequence analysis, and LSV-JDN-1 was identified as Streptomyces cavourensis. Conclusion S. alboflavus HVNDN-12 and S. cavourensis LSV-JDN-1 are actinomycete resource with great biocontrol potential against the root rot of soybean with comprehensive anti-fungi and plant growth promotion from soybean rhizosphere in black soil region.

Key words: actinomycete, antagonistic bacteria, screening, antagonism, Phytophthora sojae

CHEN Yong-qi, LI Zhi-wen, LI Xin, YUAN Ruo-xi, WANG Chun-xu, HAN Yi-qiang, GAO Ya-mei. Isolation and Function Study of Actinomycetes from Rhizosphere Soil of Soybean in the Black Soil Region[J]. Biotechnology Bulletin, 2025, 41(5): 255-266.

Figures/Tables 11

References 42

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菌株 Strain	大豆疫霉 P. sojae	尖孢镰刀菌 F. oxysporum	小麦赤霉菌 G. triticum	燕麦镰刀菌 F. oat	玉米大斑病菌 J. spot	核盘菌 S. sclerotiorum	芹菜灰霉菌 Celery cinerine mold	麦根镰刀菌 F. rhizome	稻瘟病菌 M. oryzae
LSV-JDN-1	45.84	30.67	14.74	11.16	17.88	17.53	19.55	30.98	26.98
LSV-JDN-3	44.67	30.79	27.99	20.48	20.78	19.35	17.46	17.69	27.38
GS2-JDN-7	40.27	32.03	20.61	19.52	12.48	16.35	15.20	18.34	25.56
LSV-JDN-8	42.56	27.80	15.05	14.62	14.45	18.26	24.55	27.38	35.95
LSV-JDN-10	41.58	20.74	14.70	12.64	20.75	17.68	25.64	26.10	28.39
GS-JDN-13	45.64	22.56	无	无	8.56	18.91	18.58	25.87	34.10
HV-HDN-5-1	42.87	30.69	13.26	8.64	16.58	15.24	23.13	19.35	30.64
LSV-HDN-6	40.77	20.14	12.84	18.76	13.26	19.21	20.68	25.67	31.49
HV-HDN-12	45.48	34.59	10.19	21.15	20.18	18.18	22.87	24.87	28.76
GS-HDN-12-1	43.44	34.98	20.69	25.05	22.14	16.54	15.74	11.43	23.98
GS-HDN-12-2	43.64	36.39	无	12.38	23.67	12.36	21.35	18.64	29.86

菌株 Strain	大豆疫霉 P. sojae	尖孢镰刀菌 F. oxysporum	小麦赤霉菌 G. triticum	燕麦镰刀菌 F. oat	玉米大斑病菌 J. spot	核盘菌 S. sclerotiorum	芹菜灰霉菌 Celery cinerine mold	麦根镰刀菌 F. rhizome	稻瘟病菌 M. oryzae
LSV-JDN-1	45.84	30.67	14.74	11.16	17.88	17.53	19.55	30.98	26.98
LSV-JDN-3	44.67	30.79	27.99	20.48	20.78	19.35	17.46	17.69	27.38
GS2-JDN-7	40.27	32.03	20.61	19.52	12.48	16.35	15.20	18.34	25.56
LSV-JDN-8	42.56	27.80	15.05	14.62	14.45	18.26	24.55	27.38	35.95
LSV-JDN-10	41.58	20.74	14.70	12.64	20.75	17.68	25.64	26.10	28.39
GS-JDN-13	45.64	22.56	无	无	8.56	18.91	18.58	25.87	34.10
HV-HDN-5-1	42.87	30.69	13.26	8.64	16.58	15.24	23.13	19.35	30.64
LSV-HDN-6	40.77	20.14	12.84	18.76	13.26	19.21	20.68	25.67	31.49
HV-HDN-12	45.48	34.59	10.19	21.15	20.18	18.18	22.87	24.87	28.76
GS-HDN-12-1	43.44	34.98	20.69	25.05	22.14	16.54	15.74	11.43	23.98
GS-HDN-12-2	43.64	36.39	无	12.38	23.67	12.36	21.35	18.64	29.86

实验项目 Test	LSV-JDN-1	HV-HDN-12	实验项目 Test item	LSV- JDN-1	HV-HDN-12
乳糖 Lactose	+	+	苏氨酸 Threonine	+	+
鼠李糖 Rhamnose	+	-	谷氨酰胺 Glutamine	+	+
棉子糖 Marshmallow	+	-	酪氨酸 Tyrosine	-	+
甘露醇 Mannitol	+	-	精氨酸 Argnine	+	+
葡萄糖 Glucose	+	+	丙氨酸 Alanine	+	+
木糖 Xylose	-	+	牛奶凝固与胨化 Milk peptonization	+	+
甘露糖 Mannose	+	+	明胶液化 Gelatin Liquefaction	+	+
阿拉伯糖 Arabinose	-	+	淀粉水解 Starch hydrolysis	+	+
肌醇 Inositol	+	-	硫化氢产生 Hydrogen Sulfide Generation	-	-
半乳糖 Galactose	+	+	硝酸盐还原 Nitrate Reduction	+	+
蔗糖 Fructose	+	-	纤维素水解 Urease	-	-
天冬氨酸 Aspartic acid	-	-	脲酶 Lipase	-	-
肌酸 Creatine	+	+

实验项目 Test	LSV-JDN-1	HV-HDN-12	实验项目 Test item	LSV- JDN-1	HV-HDN-12
乳糖 Lactose	+	+	苏氨酸 Threonine	+	+
鼠李糖 Rhamnose	+	-	谷氨酰胺 Glutamine	+	+
棉子糖 Marshmallow	+	-	酪氨酸 Tyrosine	-	+
甘露醇 Mannitol	+	-	精氨酸 Argnine	+	+
葡萄糖 Glucose	+	+	丙氨酸 Alanine	+	+
木糖 Xylose	-	+	牛奶凝固与胨化 Milk peptonization	+	+
甘露糖 Mannose	+	+	明胶液化 Gelatin Liquefaction	+	+
阿拉伯糖 Arabinose	-	+	淀粉水解 Starch hydrolysis	+	+
肌醇 Inositol	+	-	硫化氢产生 Hydrogen Sulfide Generation	-	-
半乳糖 Galactose	+	+	硝酸盐还原 Nitrate Reduction	+	+
蔗糖 Fructose	+	-	纤维素水解 Urease	-	-
天冬氨酸 Aspartic acid	-	-	脲酶 Lipase	-	-
肌酸 Creatine	+	+

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