Identification of the CmPOD Gene Family in Melon and Expression Analysis of Leaf Tissues under Low-temperature Stress

doi:10.13560/j.cnki.biotech.bull.1985.2025-0940

Abstract

Abstract:

Objective The class Ⅲ peroxidase (POD) gene family is involved in various biotic and abiotic stresses in plants. This study aimed to identify the CmPOD family genes and analyze their expression in leaf tissues under low-temperature stress to explore their response to cold stress. Method The thin-skinned melon variety ‘Tongtianmi 4’ was used as the material. Bioinformatics methods were employed to identify the melon POD gene family, and a systematic analysis was conducted on their physicochemical properties, phylogenetic tree, gene structure, and cis-acting elements. Protein structure prediction and expression analysis under low-temperature stress were performed for selected CmPOD genes. Result A total of 77 members of the CmPOD gene family were identified and named CmPOD1-1 to CmPOD12-7. The amino acid residue number ranged from 121 to 416 aa, and the pI value ranged from 4.53 to 9.50. Subcellular localization was mainly predicted in chloroplasts and the extracellular matrix. The CmPOD genes were unevenly distributed across 12 chromosomes, with 15 members each on Chr01 and Chr11. Phylogenetic analysis divided the CmPOD gene family into 13 subfamilies. The promoter regions were rich in stress-responsive cis-elements such as MYB, MYC, ABRE, and LTR. RT-qPCR results showed that after treatment at 4 ℃ for 24 h and 48 h, genes including CmPOD11-13, CmPOD12-6, and CmPOD11-14 were upregulated in leaves, while CmPOD7-3 was downregulated. Conclusion Seventy-seven members of the POD gene family were identified in melon, and their expression levels varied under low-temperature stress. It is speculated that the CmPOD gene family is involved in the response of melon to low-temperature stress.

Key words: melon, POD gene family, low-temperature stress, expression analysis, bioinformatics analysis

LIANG Zhi-lun, QI Si-qing, LI Xin-yang, LIANG Jian-hao, LIU Su-shuang. Identification of the CmPOD Gene Family in Melon and Expression Analysis of Leaf Tissues under Low-temperature Stress[J]. Biotechnology Bulletin, 2026, 42(10): 188-197.

Figures/Tables 10

References 31

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顺式作用元件 Cis-acting element	功能 Function
ABRE	脱落酸响应元件，参与ABA信号通路和胁迫响应
DRE core	脱水响应元件，响应低温与干旱
MYB	转录因子结合位点，参与发育、代谢和胁迫响应
MYC	转录因子结合位点，参与JA信号通路和胁迫响应
ARE	抗氧化响应元件，参与活性氧清除和氧化胁迫防御
LTR	低温响应元件，参与冷胁迫基因调控
ERE	乙烯响应元件，调控果实成熟和胁迫响应
GARE-motif	赤霉素响应元件，介导GA信号通路
AuxRR-core	生长素响应元件，调控生长素依赖基因表达
Box	光响应元件，调控光诱导基因表达
Box 4	光响应元件，参与光调控基因表达
Sp1	通用转录因子结合位点，增强基础转录
W box	WRKY转录因子结合位点，调控病原菌防御反应和SA信号通路
TC-rich repeats	防御和胁迫响应元件，参与病原体应答及机械损伤响应
MBS	MYB结合位点，参与干旱诱导基因表达
TATA-box	核心启动子元件，介导RNA聚合酶Ⅱ的转录起始定位
CAAT-box	增强子元件，提高基础转录效率
GC-motif	增强子元件，参与光诱导和胁迫响应

顺式作用元件 Cis-acting element	功能 Function
ABRE	脱落酸响应元件，参与ABA信号通路和胁迫响应
DRE core	脱水响应元件，响应低温与干旱
MYB	转录因子结合位点，参与发育、代谢和胁迫响应
MYC	转录因子结合位点，参与JA信号通路和胁迫响应
ARE	抗氧化响应元件，参与活性氧清除和氧化胁迫防御
LTR	低温响应元件，参与冷胁迫基因调控
ERE	乙烯响应元件，调控果实成熟和胁迫响应
GARE-motif	赤霉素响应元件，介导GA信号通路
AuxRR-core	生长素响应元件，调控生长素依赖基因表达
Box	光响应元件，调控光诱导基因表达
Box 4	光响应元件，参与光调控基因表达
Sp1	通用转录因子结合位点，增强基础转录
W box	WRKY转录因子结合位点，调控病原菌防御反应和SA信号通路
TC-rich repeats	防御和胁迫响应元件，参与病原体应答及机械损伤响应
MBS	MYB结合位点，参与干旱诱导基因表达
TATA-box	核心启动子元件，介导RNA聚合酶Ⅱ的转录起始定位
CAAT-box	增强子元件，提高基础转录效率
GC-motif	增强子元件，参与光诱导和胁迫响应