Identification of ZmCI-1B Promoter and Its Seven Deletions in Transgenic Arabidopsis thaliana

Abstract

Abstract: The expression vectors of ZmCI-1B promoter and its seven 5' truncated fragments were transformed into Agrobacterium tumefaciens strain GV3101. After identified by PCR assay, with Arabidopsis thaliana as genetic transformation, the expression vectors were transformed into Arabidopsis thaliana by floral-dip method. The transformed Arabidopsis thaliana plants were identified by PCR assay, then the seedings, flowers and siliques from positive plants were conducted to GUS histochemical staining. The results showed that the characterization of ZmCI-1B promoter in Arabidopsis thaliana is different from miaze. The ZmCI-1B promoter and its seven 5' truncated promoter-GUS constructs had different GUS staining in transformed Arabidopsis thaliana plants, revealing that different-length promoter had different promoting activity. The cis-acting elements on the promoter may contribute to this.

Key words: Promoter 5&apos, truncated fragments, Arabidopsis thaliana, GUS, Staining

Li Ye, Liu Xiaoqing, Li Suzhen, Zhou Xiaojin, Yang Wenzhu, Chen Rumei. Identification of ZmCI-1B Promoter and Its Seven Deletions in Transgenic Arabidopsis thaliana[J]. Biotechnology Bulletin, 2014, 0(5): 69-75.

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