植物非生物胁迫相关转录因子研究方法

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.008

摘要/Abstract

摘要： 转录因子是一类能够与启动子区域顺式作用元件特异性结合的蛋白质，是一大类转录调控因子，也是植物中最大的基因家族之一。转录因子可以调节众多下游基因的表达，对植物的生长发育、形态建成、激素调节，以及抵抗多种生物和非生物胁迫具有重要作用。结合近年来转录因子的研究进展，归纳总结了植物非生物胁迫相关转录因子研究的主要策略和方法，包括转录因子结构域、亚细胞定位、转录激活作用、转录因子复合体以及转录因子功能的研究，为植物转录因子的相关研究提供理论和方法的参考。

关键词: 非生物胁迫, 转录因子, 瞬时表达, 转录激活, 转基因植物

Abstract: Transcription factor is a kind of proteins which bind to the cis element region of the promoters specifically and a kind of transcription regulatory factors, and it is also one of the biggest gene families in plants. Many transcription factors can regulate the expression of downstream genes, and play important roles on plant growth and development, morphogenesis, hormone adjustment, and resistance to a variety of biological and abiotic stress. In combination with the research progress of transcription factors in recent years, we have summarized main strategies and methods of transcription factors to the abiotic stress, including the transcription factor domain structure, subcellular localization and transcriptional activation, transcription factor complex and functions of transcription factors, to provide related research theories and methods to plant transcription factors for reference.

Key words: abiotic stress, transcription factor, transient expression, transcriptional activation, transgenic plants

宿明星,孙颖颢,施鹤,李秋莉. 植物非生物胁迫相关转录因子研究方法[J]. 生物技术通报, 2015, 31(1): 51-60.

Su Mingxing, Sun Yinghao, Shi He, Li Qiuli. Research Methods of Abiotic Stress-related Transcription Factors in Plants[J]. Biotechnology Bulletin, 2015, 31(1): 51-60.

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