利用元基因组学方法从瘤胃中筛选的功能酶及酶基因研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.006

摘要/Abstract

摘要： 瘤胃微生物群落是一个复杂、庞大的生物体系，其生物多样性极为丰富，蕴藏着巨大的基因和生态资源，是酶制剂开发的重要宝库。元基因组学方法避免了微生物培养条件的限制，通过直接对未培养微生物进行DNA提取、基因筛选与表达，从而不断扩大自然界中的基因数据库，为新型生物催化剂的开发及筛选提供了可能。对元基因组学技术及利用此技术从瘤胃中筛选的功能酶类及酶基因的研究进展进行了综述。

关键词: 元基因组学, 瘤胃, 筛选, 酶类

Abstract: Rumen microbial community is a complex biological system with extremely rich biodiversity and large amount of genetic and ecological resources, and it is also an important treasury for the development of enzyme. Using metagenomic approach could avoid the restriction of microbial culturing, with directly extracting DNA, gene screening and expressing the uncultured microbes to enlarge the database of genes, which provides possibility for the development of novel biocatalyst. The study explained metagenomic approach, and summarized the application of metagenomic approach in screening functional enzymes and studying relevant genes from rumen.

Key words: metagenomic, rumen, screening, enzyme

张俊, 赵圣国, 王加启, 金迪, 卜登攀,. 利用元基因组学方法从瘤胃中筛选的功能酶及酶基因研究进展[J]. 生物技术通报, 2015, 31(5): 32-40.

Zhang Jun, Zhao Shengguo, Wang Jiaqi, Jin Di, Bu Dengpan. Research Progress of Enzyme and Relevant Gene Screened from Rumen by Metagenomic Approach[J]. Biotechnology Bulletin, 2015, 31(5): 32-40.

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