西瓜枯萎病菌的快速检测与鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.009

生物技术通报 ›› 2015, Vol. 31 ›› Issue (7): 58-63.doi: 10.13560/j.cnki.biotech.bull.1985.2015.07.009

西瓜枯萎病菌的快速检测与鉴定

曹月霞, 凌键, 谢丙炎, 杨宇红

(中国农业科学院蔬菜花卉研究所，北京 100081)

收稿日期:2014-11-05 出版日期:2015-07-16 发布日期:2015-07-16
作者简介:曹月霞，女，硕士研究生，研究方向：分子植物病理学；E-mail：caoyuexia815@163.com
基金资助:
国家自然科学基金项目(31272003)，国家科技支撑计划“果蔬重大病虫害防控技术研究与集成示范(2012BAD19B06)，公益性行业(农业)科研专项(200903049)，中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS)

Rapid Detection and Identification of Fusarium oxysporum f. sp. niveum

Cao Yuexia, Ling Jian, Xie Bingyan, Yang Yuhong

(Institute of Vegetable and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081)

Received:2014-11-05 Published:2015-07-16 Online:2015-07-16

摘要/Abstract

摘要： 通过西瓜枯萎病菌与其他专化型枯萎病菌及瓜类几种重要病原菌的比较基因组分析，获得了西瓜枯萎病菌的基因组特异序列。在此基础上，设计出特异引物，筛选可扩增出西瓜枯萎病菌特异性DNA条带的引物。将特异性引物和尖孢镰刀菌专化型的通用引物W106R/W106S结合，建立双重PCR检测体系。该双重PCR检测体系可以在一次PCR反应中快速、准确的检测出西瓜枯萎病菌，为通过分子方法快速鉴定西瓜枯萎病菌提供技术支持。

关键词: 西瓜枯萎病菌, 双重PCR, 尖孢镰刀菌

Abstract: Based on the genome comparison analysis between Fusarium oxysporum f. sp. niveum(Fon)and other closely-related pathogens, the specific sequences of Fon were obtained. Then we developed specific primers that could amplify Fon-specific DNA bands. Combined the Fon-specific primers with the universal primer of Fusarium oxysporum (W106R/W106S), we established a duplex PCR method that might rapidly and accurately detect Fon by 1 PCR reaction, which is beneficial to technically support the rapid identification of Fon by method of molecular detection..

Key words: Fusarium oxysporum f. sp. niveum, duplex PCR, Fusarium oxysporum

曹月霞, 凌键, 谢丙炎, 杨宇红. 西瓜枯萎病菌的快速检测与鉴定[J]. 生物技术通报, 2015, 31(7): 58-63.

Cao Yuexia, Ling Jian, Xie Bingyan, Yang Yuhong. Rapid Detection and Identification of Fusarium oxysporum f. sp. niveum[J]. Biotechnology Bulletin, 2015, 31(7): 58-63.

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西瓜枯萎病菌的快速检测与鉴定

Rapid Detection and Identification of Fusarium oxysporum f. sp. niveum

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