甘蔗己糖转运蛋白基因ShHXT6的亚细胞定位及表达

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.013

生物技术通报 ›› 2016, Vol. 32 ›› Issue (3): 79-86.doi: 10.13560/j.cnki.biotech.bull.1985.2016.03.013

甘蔗己糖转运蛋白基因ShHXT6的亚细胞定位及表达

马晓雯^1,2,赵婷婷²,王俊刚²,张树珍²,杨本鹏²

1.海南大学农学院, 海口 570228；2.中国热带农业科学院甘蔗研究中心中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开发实验, 海口 571101

收稿日期:2015-06-16 出版日期:2016-03-24 发布日期:2016-03-25
作者简介:马晓雯, 女, 硕士研究生, 研究方向：植物遗传育种, E-mail：mxwf15095555680@163.com；赵婷婷为本文并列第一作者
基金资助:
国家“863”计划(2013AA102604-1), 海南省自然科学基金项目(313078), 海南省重大科技(ZDZX2013023-1)

Subcellular Localization and Expression Analysis of Hexose Transporter Gene ShHXT6 in Sugarcane

MA Xiao-wen^{1, 2}, ZHAO Ting-ting², WANG Jun-gang², ZHANG Shu-zhen², YANG Ben-peng²

1. College of Agriculture, Hainan University, Haikou 570228；
2. Sugarcane Research Center of CATAS, Institute of Tropical Bioscience and Biotechnology of CATAS, Key Laboratory for Tropical Crops of Ministry of Agriculture, Haikou 571101

Received:2015-06-16 Published:2016-03-24 Online:2016-03-25

摘要/Abstract

摘要： 通过NCBI公布的同源物种设计特异性引物克隆出甘蔗己糖转运蛋白基因ShHXT6。该基因cDNA长1 929 bp, 其开放阅读框编码490个氨基酸。蛋白分子量为53.87 kD, 理论等电点为9.44。该基因编码的氨基酸与玉米、狗尾草和水稻的己糖转运蛋白的一致性分别为80.85%、76.89%和72.86%等。系统进化树分析表明, ShHXT6氨基酸与玉米HXT6蛋白一致性非常相近。ShHXT6在未成熟组织中表达较高, 尤其是未成熟叶, 在根中几乎不表达。构建该基因的瞬时表达载体, 通过农杆菌注射洋葱表皮的方法对ShHXT6编码的蛋白进行亚细胞定位, 结果表明, 该基因编码的蛋白定位于细胞膜。

关键词: 甘蔗, 己糖转运蛋白, 生长发育, 表达, 亚细胞定位

Abstract: The gene ShHXT6 of hexose transporter(HXT)of sugarcane was cloned with the specific primers designed according to the homologous species in NCBI. The cDNA of ShHXT6 was 1 929 bp, encoding 460 amino acids. The predicted molecular mass was 53.87 kD, and pI was 9.44. The deduced amino acid of ShHXT6 with the HXT from Zea mays, Setaria italica and Oryza sativa was in consistence by 80.85%, 76.89% and 72.86%, respectively. Phylogenetic tree analysis showed that the consistency of ShHXT6 amino acid and maize HXT6 protein was very similar. The expression of ShHXT6 was high in the immature tissues, especially in the immature leaves, but nearly no expression in the roots. The transient expression vector of this gene was constructed, and the subcellular localization of protein encoded by ShHXT6 was determined by the method of Agrobacterium injection into onion epidermis, and the results showed that the protein encoded by this gene was located in the cell membrane.

Key words: sugarcane, hexose transporter, growth and development, expression, subcelular localization

马晓雯,赵婷婷,王俊刚,张树珍,杨本鹏. 甘蔗己糖转运蛋白基因ShHXT6的亚细胞定位及表达[J]. 生物技术通报, 2016, 32(3): 79-86.

MA Xiao-wen, ZHAO Ting-ting, WANG Jun-gang, ZHANG Shu-zhen, YANG Ben-peng. Subcellular Localization and Expression Analysis of Hexose Transporter Gene ShHXT6 in Sugarcane[J]. Biotechnology Bulletin, 2016, 32(3): 79-86.

参考文献

[1]王俊刚, 赵婷婷. 甘蔗体内的蔗糖转运与运输途径[J]. 植物生物学通讯, 2008, 44(3)：605-610.
[2]Geiger D. Plant sucrose transporters from a biophysical point of view[J]. Molecular Plant, 2011, 4(3)：395.
[3]Wind J, Smeekens S, Hanson J. Sucrose：Metabolite and signaling molecule[J]. Phytochemistry, 2010, 71(14-15)：1610-1614.
[4]Dinant S, Lemoine R. The phlom pathway：new issues and old debates[J]. Comptes Rendus Biologies, 2010, 333(4)：307-319.
[5]王俊刚, 赵婷婷. 植物单糖转运蛋白[J]. 植物生物学通讯, 2007, 43(6)：1195-1198.
[6]Bush DR. Proton-coupled sugar and amino acid transport-ers in plants[J]. Annual Review of Plant Biology, 1993, 44(1)：513-542.
[7]刘珊. 质子对植物蔗糖转运蛋白和己糖转运蛋白活性的调节[J]. 黑龙江农业科学, 2012(7)：14-18.
[8]Lecourieux F, Kappel C, Lecourieux D, et al. An update on sugar transport and signalling in grapevine[J]. J Exp Bot, 2014, 65(3)：821-832.
[9]Xuan YH, Hu YB, Chen LQ, et al. Functional role of oligomerization for bacterial and plant SWEET sugar transporter family[J]. Proceedings of the National Academy of Sciences, 2013, 110(39)：E3685-E3694.
[10]Yuan M, Wang S. Rice MtN3/Saliva/SWEET family genes and their homologs in cellulLar organisms[J]. Molecular Plant, 2013, 6(3)：665-674.
[11]Chen LQ, Qu XQ, Hou BH, et al. Sucrose efflux mediated by SWEET proteins as a key step for phloem transport[J]. Science, 2012, 335：207-211.
[12]Buttner M, Saue N. Monosaccharide transporters in plants：structure, function and physiology[J]. Bochimic Biophysica Acta, 2000, 1465：263-274.
[13]Cao H, Guo S, Xu Y, et al. Reduced expression of a gene encoding a Golgi localized monosaccharide transporter(OsGMST1)confers hypersensitivity to salt in rice(Oryza sativa)[J]. Journal of Experimental Botany, 2011, 62(13)：4595-4604.
[14]Xu K, Huang X, Wu M, et al. A rapid, highly efficient and econom-ical method of Agrobacterium-mediated in planta transient transfor-mation in living onion epidermis[J]. PLoS One, 2014, 9(1)：e83556.
[15]Sauer N, Stadle R. A sink specific H⁺/monosaccharide cotransporter from Nicotoana tabacum：cloning and HXT6erologous express in baker’s yeast[J]. Plant J, 1993, 4：601-610.
[16]Truernit E, Schmid J, Epple P, et al. The sink specific and stress-regulated Arabidopsis STP4 gene：enhanced express of a gene encoding a monmsaccharide transporter by wounding, elicitor, and pathogen challengge[J]. Plant Cell, 1996, 8：2169-2182.
[17]Agasse A, Vignault C, Kappel C, et al. Sugar transport and sugar sensing in grape[M]//Roubelakis-Angelakis KA, ed. Grapevine Molecular Physiology and Biotechnology. Dordrecht：Springer, 2009：105-139.
[18]Cheng J, Wang Z, Yao F, et al. Down-regulating CsHT1, a cucumber pollen-specific hexose transporter, inhibits pollen germination, tube growth and seed development[J]. Plant Physiology, 2015, 168(2)：635-647.
[19]Martinoia E, Meyer S, De Angeli A, et al. Vacuolar transporters in their physiological context[J]. Annual Review of Plant Biology, 2012, 63：183-213.

甘蔗己糖转运蛋白基因ShHXT6的亚细胞定位及表达

Subcellular Localization and Expression Analysis of Hexose Transporter Gene ShHXT6 in Sugarcane

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