拟南芥黑芥子酶TGG1对抗旱性的作用

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.029

摘要/Abstract

摘要： 为阐明拟南芥中黑芥子酶TGG1对抗旱性的影响,构建了35S启动子驱动的TGG1过表达载体,并将其转入拟南芥获得了转基因植株。以野生型和过量表达TGG1的转基因植株为材料,进行干旱胁迫实验,结果显示,在甘露醇模拟的干旱胁迫下35S∶TGG1种子平均发芽率显著高于野生型,平均相对电导率则显著低于野生型;自然干旱胁迫下,35S∶TGG1的相对失水速率显著慢于野生型,而干旱复水后的平均存活率则显著高于野生型。对气孔的观察结果表明,过表达TGG1的转基因植株气孔对ABA处理具有更高的敏感性,气孔关闭程度显著高于野生型植株。以上研究结果表明,过量表达TGG1基因可显著提高拟南芥的抗旱能力,而且其抗性机制很可能与气孔在逆境下的关闭程度有关。

关键词: 芥子油苷水解酶, TGG1, 拟南芥, 干旱胁迫, 气孔

Abstract: In order to illuminate the function of myrosinase TGG1 in drought resistance,an over-expressing vector with TGG1 gene driven by 35S promoter was constructed and transferred into Arabidopsis thaliana. Drought stress were tested using the wild and TGG1-overexpressed transgenic plants. Our results showed that in a simulated drought stress by the treatment of mannitol,the seed germination rate of transgenic seedlings was significantly higher than those of wild type,while relative conductivity rate of leaves in transgenic plants was significantly lower than that in wild type. In the condition of natural drought stress followed by re-watering,35S∶TGG1 showed higher survival rate and was more sensitive to ABA in the process of stomatal closure,and consequently presented the decreased water loss rate and permeability of cell membrane. In general,our study suggested that overexpressed TGG1 gene improved the drought tolerance of A. thaliana,and the mechanism is likely related to degree of stomata closing in stress.

Key words: myrosinase, TGG1, Arabidopsis thaliana, drought stress, stomata

毛明宇, 张文宇, 夏洪宇, 王洋, 李晶. 拟南芥黑芥子酶TGG1对抗旱性的作用[J]. 生物技术通报, 2016, 32(9): 218-224.

MAO Ming-yu¹, ZHANG Wen-yu¹, XIA Hong-yu¹, WANG Yang², LI Jing¹. The Role of Myrosinase TGG1 in Drought Resistance in Arabidosis thaliana[J]. Biotechnology Bulletin, 2016, 32(9): 218-224.

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