干旱胁迫下蒙古沙冬青叶片蛋白质组学研究

doi:10.13560/j.cnki.biotech.bull.1985.2017-0012

摘要/Abstract

摘要： 通过对蒙古沙冬青[Ammopiptanthus mongolicus（Maxim）Cheng. f]叶组织干旱胁迫下蛋白质组变化的研究,从蛋白表达水平阐释其应答干旱胁迫的分子机制。以20% PEG 6000胁迫处理1 h和72 h,以0 h为对照,提取叶片总蛋白,利用双向电泳和质谱鉴定技术分析差异表达蛋白。共鉴定了40个差异表达蛋白,按功能可分为9类：光合作用,ROS清除,蛋白的合成、加工与降解,物质运输,防御相关,RNA加工,氨基酸代谢,其他相关蛋白和功能未知蛋白质。蒙古沙冬青叶片应答干旱胁迫的核心是叶绿体结构和光合作用的维持。

关键词: 蒙古沙冬青, 叶片, 蛋白质组, 差异表达蛋白

Abstract: The aim of the present study is to reveal the proteomic variation of Ammopiptanthus mongolicus（Maxim）Cheng. f leaves under drought stress. The plants were exposed to 20% PEG 6000 for 1 and 72 h,and 0 h as control. The extracted leaves proteins of the treatment and the control were analyzed by two-dimensional electrophoresis（2-DE）,and the differentially expressed proteins were identified by MALDI-TOF-TOF. In total,40 differentially expressed proteins were detected. These identified proteins were mainly involved in photosynthesis,ROS scavenging,protein synthesis,processing and degradation,transport,defense related,RNA processing,amino acid metabolism,other and unknown function. Conclusively,the key of A. mongolicus response to drought stress was the maintenance of structure and photosynthesis of chloroplasts.

Key words: Ammopiptanthus mongolicus, leaf, proteome, differentially expressed proteins

付晨熙, 肖自华, 高飞, 周宜君. 干旱胁迫下蒙古沙冬青叶片蛋白质组学研究[J]. 生物技术通报, 2017, 33(6): 69-80.

FU Chen-xi, XIAO Zi-hua, GAO Fei, ZHOU Yi-jun. Proteomics Analysis of Ammopiptanthus mongolicus Leaves Under Drought Stress[J]. Biotechnology Bulletin, 2017, 33(6): 69-80.

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