小桐子转化酶基因家族的生物信息学分析及表达特性

doi:10.13560/j.cnki.biotech.bull.1985.2017-0303

生物技术通报 ›› 2017, Vol. 33 ›› Issue (9): 172-178.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0303

小桐子转化酶基因家族的生物信息学分析及表达特性

王海波,辛胡,刘潮,唐利洲

曲靖师范学院云南高原生物资源保护与利用研究中心云南省高校云贵高原动植物遗传多样性及生态适应性重点实验室,曲靖 655011

收稿日期:2017-04-14 出版日期:2017-09-01 发布日期:2017-09-15
作者简介:王海波,男,博士,副教授,硕士生导师,研究方向：植物逆境分子生物学；E-mail：bocai0406@163.com
基金资助:
国家自然科学基金项目（31460179）

Genome-wide Identification and Expression Characteristics of Invertase Gene Family in Jatropha curcas

WANG Hai-bo,XIN Hu,LIU Chao,TANG Li-zhou

Center for Yunnan Plateau Biological Resources Protection and Utilization,Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yungui Plateau,Qujing Normal University,Qujing 655011

Received:2017-04-14 Published:2017-09-01 Online:2017-09-15

摘要/Abstract

摘要： 转化酶是植物蔗糖代谢与抗逆性形成的关键酶。基于小桐子基因组数据,鉴定到16个小桐子转化酶基因,并对其系统进化、基因结构、理化性质、保守基序及表达特性进行了分析。结果表明,小桐子转化酶基因家族聚类为3个亚家族,中性/碱性转化酶α亚组与酸性转化酶基因包含6或7个外显子,β亚组包含4或5个外显子。同时,酸性转化酶N端都包含信号肽序列,且都鉴定到GH32家族保守的-NDPNG/A-与-MWECV/P-基序。qRT-PCR表达分析显示,JcC-INVD基因在小桐子子叶中表达量最高,而在根中表达量较低,且在叶中属于低温诱导基因。

关键词: 小桐子, 转化酶, 基因家族, 表达分析

Abstract: Plant invertase is one of critical enzymes in sucrose metabolism and stress resistance formation. The 16 invertase genes were identified based on complete genome data of Jatropha curcas,then the evolutionary relationship,gene structure,physical and chemical characteristics,conserved motif,and expression feature were analyzed. The results showed that invertase gene family in J. curcas was clustered to three subfamilies according to phylogenetic analysis. The α subgroup of neutral/alkaline invertases and acidic invertase gene owned 6-7 exons,and β subgroup owned 4-5 exons in gene structure. Signal peptides in N-terminal and conserved motifs of -NDPNG/A- and -MWECV/P- belonging to GH32 family were identified in acidic invertase protein sequences. qRT-PCR analysis revealed that JcC-INVD expressed differently in different tissues,with abundantly in cotyledon,but scarcely in root,and remarkably cold-induced expression in leaf.

Key words: Jatropha curcas, invertase, gene family, expression analysis

王海波,辛胡,刘潮,唐利洲. 小桐子转化酶基因家族的生物信息学分析及表达特性[J]. 生物技术通报, 2017, 33(9): 172-178.

WANG Hai-bo,XIN Hu,LIU Chao,TANG Li-zhou. Genome-wide Identification and Expression Characteristics of Invertase Gene Family in Jatropha curcas[J]. Biotechnology Bulletin, 2017, 33(9): 172-178.

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小桐子转化酶基因家族的生物信息学分析及表达特性

Genome-wide Identification and Expression Characteristics of Invertase Gene Family in Jatropha curcas

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