生物技术通报 ›› 2017, Vol. 33 ›› Issue (10): 143-147.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0536

• 研究报告 • 上一篇    下一篇

根皮苷降解真菌的筛选、鉴定及降解特性研究

刘淑艳1, 王芳2, 王建宇2, 林榕姗2   

  1. 1. 辽宁出入境检验检疫局,大连 116015
    2. 山东农业大学,泰安 271018
  • 收稿日期:2017-06-29 出版日期:2017-10-29 发布日期:2017-10-29
  • 作者简介:刘淑艳,女,博士,研究方向:食品微生物学及分子生物学;E-mail:Liusy@lnciq.gov.cn
  • 基金资助:
    国家“948”重点项目(2011-G30),国家质检公益课题(201310230),产毒真菌分子生物学快速检测体系的建立(2010IK131),“十三五”国家重点研发计划课题(SQ2017ZY060105-06)

Isolation,Identification and Degrading Properties of Phlorizin-Degrading Fungi

LIU Shu-yan1, WANG Fang2, WANG Jian-yu2, LIN Rong-shan2   

  1. 1. Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116015
    2. Shan Dong Agricultural University,Taian 271018
  • Received:2017-06-29 Published:2017-10-29 Online:2017-10-29

摘要: 筛选高效降解根皮苷的功能菌以期为生物降解根皮苷、缓解苹果连作障碍奠定基础。采用以根皮苷为唯一碳源选择性培养基筛选菌种,对筛选的菌种进行生理生化和分子生物学鉴定,并研究了不同培养条件下的降解特性。结果显示,从环渤海苹果主产区的12个县市地区的苹果根际土壤中筛选出一株能够高效降解根皮苷的菌株AMCC300110,经形态学和分子生物学方法初步鉴定该菌为土曲霉(Aspergillus terreus),该菌在根皮苷初始浓度为2 mmol/L、pH5.0,接种量2%条件下,40℃、160 r/min摇床培养96 h,降解率可达88.96%。降解特性研究结果表明,不同培养条件下摇床培养12 h,该菌分别在pH为5.0,培养温度40℃,初始根皮苷浓度为6 mmol/L时有最大的降解率,同时该菌还具有广谱降解酚酸的能力。通过选择性培养基筛选得到一株土曲霉,该菌具有高效降解根皮苷的能力,在生物降解酚酸类自毒物质,缓解苹果连作障碍方面有一定的应用潜力。

关键词: 根皮苷, 生物降解, 连作障碍, 土曲霉

Abstract: Screening highly efficient phlorizin-degrading microorganism is important for bioremediation of phlorizin-contaminated environment. This study aimed to isolate and identify high efficient phlorizin-degrading microorganism from the rhizosphere soils of apple,then to characterize degrading properties of target strains. Phlorizin-degrading microorganism were screened by the plate-culture method,used phloridzin as the sole carbon sources,and then the target strains were identified by the morphological and molecular methods. Degrading properties of phlorizin -degrading microorganism were determined under different condition of culture. The results showed that a phlorizin-degrading fungal strain AMCC300100 identified as Aspergillus terreus were obtained from the rhizosphere soils of apple replantd and non-replanted orchards surrounding Bohai gulf area. The phlorizin degradation rate of the strain AMCC300100 has reached 88.96%(phlorizin initial concentration of 2 mmol/L,pH5.0,2% inoculum)cultured 96h under the conditions of 40℃,160r/min;The strain AMCC300110 have high-efficient degradation ability not only for phlorizim but also for the other phenolic acids. Though plate-culture method,We obtained a fungal strain identified as Aspergillus terreus,this fungal strain have high-efficient degradation ability for phlorizim,and have tremendous potential in solving apple replant disease.

Key words: phlorizin, biodegradation, replant disease, Aspergillus terreus