4-香豆酸辅酶A连接酶响应大豆孢囊线虫胁迫的潜在功能

doi:10.13560/j.cnki.biotech.bull.1985.2021-0378

摘要/Abstract

摘要：

大豆孢囊线虫是大豆产区病虫害防治策略的重要目标之一,大豆孢囊线虫的防控也一直是线虫领域研究热点之一。大豆孢囊线虫侵染不仅会造成大豆地下部分损伤,也使得地上部分受损从而影响其产量,因此需对大豆孢囊线虫的抗性机制进行分析以达到防控的目的。大豆孢囊线虫成功寄生宿主植物需对其细胞壁进行降解融合,形成为其生长发育提供唯一营养来源的合胞体。而阻碍大豆孢囊线虫移动和合胞体建立的细胞壁抗性是大豆抵御大豆孢囊线虫的关键,其中木质素是细胞壁发挥抗性的重要成分。木质素的生物合成主要包括莽草酸代谢途径、苯丙烷代谢途径和木质素合成的特异途径,4-香豆酸辅酶A连接酶（4-coumarate-Coenzyme A ligase,4CL）作为连接苯丙烷代谢途径和木质素特异合成途径的重要转折酶,决定了木质素的合成,其很可能是响应大豆孢囊线虫胁迫的重要调控因子。本文从线虫入侵需要细胞壁降解融合建立合胞体出发,围绕木质素导致的细胞壁抗性展开讨论,分析了4CL在细胞壁抗性中的响应机制,为进一步探索大豆孢囊线虫胁迫机制提供科学依据。

关键词: 大豆孢囊线虫, 合胞体, 木质素, 细胞壁, 4-香豆酸辅酶A连接酶

Abstract:

Soybean cyst nematode（SCN,Heterodera glycines）is one of the important targets of pest control strategies in soybean production areas. The prevention and control of SCN has always been one of the hot spots in the field of nematodes. SCN infection not only damage the underground part of soybean plant,but also damage the above ground part and thus affects soybean yield. Therefore,it is necessary to analyze the SCN resistance mechanism in order to achieve the purpose of prevention and control. That SCN successfully parasitizes host plants requires degradation and fusion of its cell wall to form a syncytium that provides the only source of nutrients for its growth and development. The cell wall resistance that hinders the movement of SCN and the establishment of syncytia is the key for soybean to resist SCN,while lignin is an important component of cell wall resistance. The biosynthesis of lignin mainly includes shikimic acid metabolic pathway,phenylpropane metabolic pathways,and specific pathways for lignin synthesis,4-coumarate-coenzyme A ligase（4CL）,as an important turning enzyme connecting the phenylpropane metabolic pathway and lignin-specific synthesis pathway,determines the synthesis of lignin,which may be an important regulatory factor in response to SCN stress. This article starts from the nematode invasion that requires cell wall degradation and fusion to establish syncytium,discusses the cell wall resistance caused by lignin,analyzes the response mechanism of 4CL in cell wall resistance,and provides a scientific basis for further exploring the SCN stress mechanism.

Key words: soybean cyst nematode, syncytium, lignin, cell wall, 4 coumaric acid-CoA ligase

王惠, 张顺斌, 金贺, 王晗, 张耕华, 夏诗宁, 陈井生, 段玉玺. 4-香豆酸辅酶A连接酶响应大豆孢囊线虫胁迫的潜在功能[J]. 生物技术通报, 2021, 37(7): 71-80.

WANG Hui, ZHANG Shun-bin, JIN He, WANG Han, ZHANG Geng-hua, XIA Shi-ning, CHEN Jing-sheng, DUAN Yu-xi. Potential Function of 4-coumaric Acid-CoA Ligase in Response to Soybean Cyst Nematode Stress[J]. Biotechnology Bulletin, 2021, 37(7): 71-80.

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