植物线粒体和叶绿体RNA编辑的比较

生物技术通报 ›› 2013, Vol. 0 ›› Issue (3): 90-95.

植物线粒体和叶绿体RNA编辑的比较

万平

首都师范大学生命科学学院，北京 100048

收稿日期:2012-10-24 修回日期:2013-03-21 出版日期:2013-03-20 发布日期:2013-03-21
作者简介:万平，男，博士，研究方向：生物信息学；E-mail ：wanp_cnu@yahoo.com.cn

Comparasion of RNA Editing in Plant Mitochondria and Chloroplasts

Wan Ping

College of Life Science,Capital Normal University,Beijing 100048）

Received:2012-10-24 Revised:2013-03-21 Published:2013-03-20 Online:2013-03-21

摘要/Abstract

摘要： 植物中RNA 编辑发生在叶绿体和线粒体两种细胞器中，通过对非维管植物（角苔）和维管植物（蕨类和被子植物）线粒体中4 892 个RNA 编辑位点和叶绿体中1 228 个RNA 编辑位点的（1）氨基酸转移概率、（2）密码子转移概率、（3）编辑位点在密码子中出现位置概率、（4）编辑位点-1 位碱基出现概率、（5）编辑位点+1 位碱基出现概率5 个方面进行比较分析。结果发现，被子植物中线粒体和叶绿体RNA 编辑在氨基酸转移概率、密码子转移概率方面存在显著差异。

关键词: 非维管植物, 维管植物, 线粒体, 叶绿体, RNA, 编辑

Abstract: In plants, the process of RNA editing occurs in organelles chloroplasts and mitochondria. In this work, we analyzed the 4 892 mitochondrial RNA editing sites and 1 228 chloroplastic RNA editing sites of non-vascular and vascular land plants in features of（1）the probabilities of amino acid transitions, （2）the probabilities of codon transitions, （3）the probabilities of positions of editing site occurring in the codon, （4）the probabilities of the occurrence of four bases at the minus 1 position near the RNA editing site, and（5）the probabilities of the occurrence of four bases at the plus 1 position near the RNA editing site. We find that in eudicot, the probabilities of amino acid transitions and the probabilities of codon transitions are both significantly different between mitochondrial and chloroplast RNA editing.

Key words: Non-vascular land plants, Vascular land plants, Mitochondrion, Chloroplast, RNA editing

万平. 植物线粒体和叶绿体RNA编辑的比较[J]. 生物技术通报, 2013, 0(3): 90-95.

Wan Ping. Comparasion of RNA Editing in Plant Mitochondria and Chloroplasts [J]. Biotechnology Bulletin, 2013, 0(3): 90-95.

参考文献

[1] Gott JM. Expanding genome capacity via RNA editing[J]. Comptes Rendus Biologies, 2003, 326(10-11):901-908.
[2] Brennicke A, Marchfelder A, Binder S. RNA editing
[K]. FEMS Microbiol Rev, 1999, 23(3):297-316.
[3] Knoop V. When you can’t trust the DNA :RNA editing changes transcript sequences[J]. Cell Mol Life Scie, 2011, 68 :1-20.
[4] Benne R, Van den Burg J, Brakenhoff JP, et al. Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA[J]. Cell, 1986, 46(6):819.
[5] Covello PS, Gray MW. RNA editing in plant mitochondria[J]. Nature, 1989, 341 :662-666.
[6] Hiesel R, Wissinger B, Schuster W, Brennicke A. RNA editing in plant mitochondria[J]. Science, 1989, 246 :1632-1634.
[7] Gualberto JM, Lamattina L, Bonnard G, et al. RNA editing in wheat mitochondria results in the conservation of protein sequences[J]. Nature, 1989, 341(6243):660-662.
[8] Hoch B, Maier RM, Appel K, et al. Editing of a chloroplast mRNA by creation of an initiation codon[J]. Nature, 1991, 353 :178-180.
[9] Grosjean H. Modification and editing of RNA :historical overview and important facts to remember[J]. Topics in Current Genetics, 2005, 12 :1-22.
[10] Chateigner-Boutin AL. Plant RNA editing[J]. RNA Biology, 2010, 7(2):213-219.
[11] Freyer R, Kiefer-Meyer MC, K?ssel H. Occurrence of plastid RNA editing in all major lineages of land plants[J]. PNAS, 1997, 94 (12):6285- 6290.
[12] Pfitzinger H, Weil JH, Pillay DTN, Guillemaut P. Codon recognition mechanisms in plant chloroplasts[J]. Plant Molecular Biology, 1990, 14(5):805-814.
[13] Delannoy E, Le Ret M, Faivre-Nitschke E, et al. Arabidopsis tRNA adenosine deaminase arginine edits the wobble nucleotide of chloroplast tRNAArg(ACG)and is essential for efficient chloroplast translation[J]. The Plant Cell, 2009, 21(7):2058-2071.
[14] Gray MW. RNA editing in plant mitochondria:20 years later[J]. IUBMB Life, 2009, 61(12):1101-1104.
[15] Kotera E, Tasaka M, Shikanai T. A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts[J]. Nature, 2005, 433(7023):326-330.
[16] Fujii S, Small I. The evolution of RNA editing and pentatricopeptide repeat genes[J]. New Phytol, 2011, 191(1):37-47.
[17] Thompson J, Gopal S. Genetic algorithm learning as a robust approach to RNA editing site prediction[J]. BMC Bioinformatics, 2006, 7(1):145.
[18] Thompson J, Gopal S. Correction :genetic algorithm learning as a robust approach to RNA editing site prediction[J]. BMC Bioinformatics, 2006, 7(1):406.
[19] Gray MW. Evolutionary origin of RNA editing[J]. Biochemistry, 2012, 51(26):5235-5242.
[20] Marechal-Drouard L, Weil JH, Guillemaut P. Import of several tRNAs from the cytoplasm into the mitochrondria in bean Phaseolus vulgaris[J]. Nucl Acids Res, 1988, 16(11):4777-4788.
[21] Ohyama K, Fukuzawa H, Kohchi T, et al. Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA[J]. Nature, 1986, 322 :572-574.
[22] Shinozaki K, Ohme M, Tanaka M, et al. The complete nucleotide sequence of the tobacco chloroplast genome :its gene organization and expression[J]. The EMBO Journal, 1986, 5(9):2043-2049.
[23] Takenaka M, Zehrmann A, Verbitskiy D, et al. Multiple organellar RNA editing factor(MORF)family proteins are required for RNA editing in mitochondria and plastids of plants[J]. PNAS, 2012, 109(13):5104-5109.