栀子ERF基因的克隆及其在果实成熟过程中的表达

摘要/Abstract

摘要： 乙烯反应元件因子（ethylene-responsive element-binding factor，ERF）是一类植物DNA 结合蛋白，是乙烯信号传导过程中的一类转录因子，与植物的生长发育和生理过程有关。从栀子（Gardenia jasminoides Ellis）果实的cDNA文库中筛选获得栀子乙烯反应元件因子GjERF的cDNA。该基因全长962 bp，5'端非翻译区长82 bp，3'端非翻译区长100 bp，预测ORF为780 bp，编码259个氨基酸，分子量为28.6 kD。序列分析表明GjERF含有59个氨基酸构成的AP2/ERF结构域及N端的MC（MCGGAII）模体，它属于ERF家族的第Ⅶ亚类。RT-PCR分析表明GjERF基因在栀子成熟叶片中的表达高于在果实中的表达，并且在果实中的表达与果实的成熟过程无关。

关键词: 栀子, 乙烯反应元件反应因子, 克隆, 表达

Abstract: Ethylene-responsive element-binding factor（ERF）genes constitute one of the DNA-binding protein gene families in plants, and are transcription factors associated with ethylene signal transduction, developmental and physiological processes. In this paper, a new member of the AP2/ERF transcription factor family, GjERF, was isolated from Gardenia jasminoides fruit cDNA library. The GjERF cDNA is 962 bp, contains a predicated 780 bp open reading frame that encodes a protein of 259 amino acids with calculated molecular mass of 28.6 kD, and with a 82 bp non-coding region at 5' end, a 100 bp of non-coding region flank at 3' end. Sequence analysis showed that GjERF contained an AP2/ERF domain of 59 amino acids and a N- terminal MC motif（MCGGAII）. It belonged to a group Ⅶ protein in the ERF subfamily. The RT-PCR analysis indicated that the transcription of GjERF gene accumulated primarily in mature leaves of G.jasminoides, and was not related with fruit developmental stages.

Key words: Gardenia jasminoides, ERF, Clone, Expression

高蓝,卢静雯. 栀子ERF基因的克隆及其在果实成熟过程中的表达[J]. 生物技术通报, 2013, 0(7): 60-65.

Gao Lan, Lu Jingwen. Molecular Cloning and Expression Analysis of ERF Transcription Factor Gene in Gardenia jasminoides During Fruit Development [J]. Biotechnology Bulletin, 2013, 0(7): 60-65.

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