逆境胁迫下植物表观遗传机制的研究进展

摘要/Abstract

摘要：

植物着地固定生长不能主动逃避外界危害，只能依靠自身的一些响应机制来防御外界胁迫，表观遗传调控在这个响应机制中起着重要的作用，主要表现在DNA甲基化、组蛋白修饰、染色质重塑及非编码RNA。植物在遭受低温、高温、干旱、盐、重金属、病毒及激素等因素胁迫后，通过调节抗逆相关基因的表达来响应外界危害。综述表观遗传修饰在各种胁迫下的调控机制，为作物的抗逆研究提供理论依据。

关键词: 胁迫, DNA甲基化, 组蛋白修饰, 染色质重塑, 非编码RNA

Abstract:

Plant as sedentary organisms, needs to adapt their gene activity to the adverse or stressful environmental challenges. Epigenetic regulation accompanies stressful environments, such as extreme temperature, drought, salinity, heavy metal, pathogen and hormones etc., which lead to the impressive development and phenotype variation of different plant species with adaptability to unfavorable conditions. In this paper, the current research status of epigenetic changes induced by stresses, including DNA methylation, histone post-translational modification, chromatin modification, non-coding RNA, as well as the interaction between these epigenetic incidences were reviewed.

Key words: Stress, DNA methylation, Histone modification, Chromatin reshaping, Non-coding RNA

冉莉萍, 孔月琴, 方婷婷, 王幼平. 逆境胁迫下植物表观遗传机制的研究进展[J]. 生物技术通报, 2014, 0(8): 8-15.

Ran Liping, Kong Yueqin, Fang Tingting, Wang Youping. Research Progresses of Stress-induced Epigenetic Regulation Mechanism in Plant[J]. Biotechnology Bulletin, 2014, 0(8): 8-15.

参考文献

［1］Dyachenko OV, Zakharchenko NS, Shevchuk TV, et al. Effect of hy-permethylation of CCWGG sequences in DNA of Mesembryanthemum crystallinum plants on their adaptation to salt stress［J］. Bioche-mistry, 2006, 71（4）：461-465.
［2］Veiseth SV, Rahman MA, Yap KL, et al. The SUVR4 histone lysine methyltransferase binds ubiquitin and converts H3K9me1 to H3K9me3 on transposon chromatin in Arabidopsis［J］. PLoS Genet, 2011, 7（3）：e1001325.
［3］Karan R, DeLeon T, Biradar H, et al. Salt stress induced variation in DNA methylation pattern and its influence on gene expression in contrasting rice genotypes［J］. PLoS One, 2012, 7（6）：e40203.
［4］Finnegan EJ. Epialleles-a source of random variation in times of stress［J］. Curr Opin Plant Biol, 2001, 5（2）：101-106.
［5］Chinnusamy V, Zhu JK. Epigenetic regulation of stress responses in plants［J］. Curr Opin Plant Biol, 2009, 12（2）：133-139.
［6］Zhang K, Sridhar VV, Zhu JH, et al. Distinctive core histone post-translational modification patterns in Arabidopsis thaliana［J］. PLoS One, 2007, 2（11）：e1210.
［7］Tittel-Elmer M, Bucher B, Broger L, et al. Stress-induced activation of heterochromatic transcription［J］. PLoS Genet, 2010, 6（10）：e1001175.
［8］Finnegan EJ, Kovac KA. Plant DNA methyltransferases［J］. Plant Mol Biol, 2000, 43（2-3）：189-201.
［9］Miguel C, Marum L. An epigenetic view of plant cells cultured in vitro：somaclonal variation and beyond［J］. J Exp Bot, 2011, 62（11）：3713-3725.
［10］Steward N, Kusano T, Sano H. Expression of ZmMET1, a gene encoding a DNA methyltransferase from maize, is associated not only with DNA replication in actively proliferating cells, but also with altered DNA methylation status in cold-stressed quiescent cells［J］. Nucl Acids Res, 2000, 28（17）：3250-3259.
［11］Law JA, Jacobsen SE. Establishing, maintaining and modifying DNA methylation patterns in plants and animals［J］. Nat Rev Genet, 2010, 11（3）：204-220.
［12］Zilberman D, Henikoff S. Epigenetic inheritance in Arabidopsis：selective silence［J］. Curr Opin Genet Dev, 2005, 15（5）：557-562.
［13］Choi CS, Sano H. Abiotic-stress induces demethylation and transc-riptional activation of a gene encoding a glycerophosphodiesterase-like protein in tobacco plants［J］. Mol Genet Genomics, 2007, 2779（5）：589-600.
［14］Bhardwaj J, Mahajan M, Yadav SK. Comparative analysis of DNA methylation polymorphism in drought sensitive（HPKC2）and tolerant（HPK4）genotypes of horse gram（Macrotyloma uniflorum）［J］. Biochem Genet, 2013, 51（7-8）：493-502.
［15］Gayacharan, Joel AJ. Epigenetic responses to drought stress in rice（Oryza sativa L.）［J］. Physiol Mol Biol Plants, 2013, 19（3）：379-87.
［16］Wang WS, Pan YJ, Zhao XQ, et al. Drought-induced site-specific DNA methylation and its association with drought tolerance in rice（Oryza sativa L.）［J］. J Exp Bot, 2011, 62（6）：1951-1960.
［17］Angers B, Castonguay E, Massicotte R. Environmentally induced phenotypes and DNA methylation：how to deal with unpredictable conditions until the next generation and after［J］. Mol Ecol, 2010, 19（7）：1283-1295.
［18］Wada Y, Miyamoto K, Kusano T, et al. Association between up-regulation of stress-responsive genes and hypomethylation of genomic DNA in tobacco plants［J］. Mol Genet Genomics, 2004, 271（6）：658-666.
［19］Muthamilarasan M, Prasad M. Plant innate immunity：an updated insight into defense mechanism［J］. J Biosci, 2013, 38（2）：433-449.
［20］Dowen RH, Pelizzola M, Schmitz RJ, et al. Widespread dynamic DNA methylation in response to biotic stress［J］. Proc Natl Acad Sci USA, 2012, 109（32）：2183-2191.
［21］Wang YS, An CF, Zhang XD, et al. The Arabidopsis elongator complex subunit 2 epigenetically regulates plant immune responses［J］. Plant Cell, 2013, 25（2）：762-776.
［22］Yadav RK, Chattopadhyay D. Enhanced viral intergenic region-specific short interfering RNA accumulation and DNA methylation correlates with resistance against a geminivirus［J］. Mol Plant Microbe Interact, 2011, 24（10）：1189-1197.
［23］Boyko A, Kathiria P, Zemp FJ, et al. Transgenerational changes in the genome stability and methylation in pathogen-infected plants：（virus-induced plant genome instability）［J］. Nucl Acids Res, 2007, 35（5）：1714-1725.
［24］Li XY, Wang XF, He K, et al. High-resolution mapping of epigenetic modifications of the rice genome uncovers between DNA methylation, histone methylation, and gene expression［J］. Plant Cell, 2008, 20（2）：259-276.
［25］Amente S, Bertoni A, Morano A, et al. LSD1-mediated demethyla-tion of histone H3 lysine 4 triggers myc-induced transcription［J］. Oncogene, 2010, 29（25）：3691-3702.
［26］Liu CY, Lu FL, Cui X, et al. Histone methylation in higher plants［J］. Annu Rev Plant Biol, 2010, 61：395-420.
［27］Kim JM, To TK, Ishida J, et al. Alterations of lysine modifications on the histone H3 N-tail under drought stress conditions in Arabidopsis thaliana［J］. Plant Cell Physiol, 2008, 49（10）：1580-1588.
［28］Scippa GS, Di Michele M, Onelli E, et al. The histone-like protein H1-S and the response of tomato leaves to water deficit［J］. J Exp Bot, 2004, 55（394）：99-109.
［29］Tsuji H, Saika H, Tsutsumi N, et al. Dynamic and reversible changes in histone H3-Lys4 methylation and H3 acetylation occurring at submergence-inducible genes in rice［J］. Plant Cell Physiol, 2006, 47（7）：995-1003.
［30］Jiang DH, Wang YQ, Wang YZ, et al. Repression of FLOWERING LOCUS C and FLOWERING LOCUS T by the Arabidopsis poly-comb repressive complex 2 components［J］. PLoS One, 2008, 3（10）：e3404.
［31］Strahl BD, Allis CD. The language of covalent histone modifica-tions［J］. Nature, 2000, 403（6765）：41-45.
［32］Kim JM, To TK, Seki M. An epigenetic integrator：new insights into genome regulation, environmental stress responses and developmental controls by histone deacetylase 6［J］. Plant Cell Physiol, 2012, 53（5）：794-800.
［33］To TK, Nakaminami K, Kim JM, et al. Arabidopsis HDA6 is requ-ired for freezing tolerance［J］. Biochem Biophys Res Commun, 2011, 406（3）：414-419.
［34］Zhu JH, Jeong JC, Zhu YM, et al. Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance［J］. Proc Natl Acad Sci USA, 2008, 105（12）：4945-4950.
［35］Alvarez ME, Nota F, Cambiagno DA. Epigenetic control of plant immunity［J］. Mol Plant Pathol, 2010, 11（4）：563-576.
［36］Kim KC, Lai ZB, Fan BF, et al. Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense［J］. Plant Cell, 2008, 20（9）：2357-2371.
［37］Chen LT, Wu KQ. Role of histone deacetylases HDA6 and HDA19 in ABA and abiotic stress response［J］. Plant Signal Behav, 2010, 5（10）：1318-1320.
［38］Sridha S, Wu K. Identification of AtHD2C as a novel regulator of abscisic acid responses in Arabidopsis［J］. Plant J, 2006, 46（1）：124-133.
［39］Park HJ, Kim WY, Park HC, et al. SUMO and SUMOylation in plants［J］. Mol Cells, 2011, 32（4）：305-316.
［40］Hu Y, Zhu N, Wang X, et al. Analysis of rice Snf2 family proteins and their potential roles in epigenetic regulation［J］. Plant Physiol Biochem, 2013, 70：33-42.
［41］Efroni I, Han SK, Kim HJ, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses［J］. Cell, 2013, 24（4）：438-445.
［42］Saez A, Rodrigues A, Santiago J, et al. HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis［J］. Plant Cell, 2008, 20（11）：2972-2988.
［43］Rios G, Gagete AP, Castillo J, et al. Abscisic acid and desiccation-dependent expression of a novel putative SNF5-type chromatin-remodeling gene in Pisum sativum［J］. Plant Physiol Biochem, 2007, 45（6-7）：427-435.
［44］Jeddeloh JA, Stokes TL, Richards EJ. Maintenance of genomic methylation requires a SWI2/SNF2-like protein［J］. Nat Genet, 1999, 22（1）：94-97.
［45］Vongs A, Kakutani T, Martienssen RA, et al. Arabidopsis thaliana DNA methylation mutants［J］. Science, 1993, 260（5116）：1926-1928.
［46］Yao YL, Bilichak A, Golubov A, et al. ddm1 plants are sensitive to methyl methane sulfonate and NaCl stresses and are deficient in DNA repair［J］. Plant Cell Rep, 2012, 31（9）：1549-1561.
［47］March-Díaz R, García-Domínguez M, Florencio FJ, et al. SEF, a new protein required for flowering repression in Arabidopsis, interacts with PIE1 and ARP6［J］. Plant Physiol, 2007, 143（2）：893-901.
［48］Sunkar R, Kapoor A, Zhu JK. Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by down-regulation of miR398 and important for oxidative stress tolerance［J］. Plant Cell, 2006, 18（8）：2051-2065.
［49］Contreras-Cubas C, Palomar M, Arteaga-Vazquez M, et al. Non-coding RNAs in the plant response to abiotic stress［J］. Planta, 2012, 236（4）：943-958.
［50］Mendoza-Soto AB, Sanchez F, Hernandez G. MicroRNAs as regulators in plant metal toxicity response［J］. Front Plant Sci, 2012, 3：105.
［51］Dugas DV, Bartel B. Sucrose induction of Arabidopsis miR398 represses two Cu/Zn superoxide dismutases［J］. Plant Mol Biol, 2008, 67（4）：403-417.
［52］Khraiwesh B, Zhu JK, Zhu JH. Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants［J］. Biochim Biophys Acta, 2012, 1819（2）：137-148.
［53］Liu PP, Montgomery TA, Fahlgren N, et al. Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages［J］. Plant J, 2007, 52（1）：133-146.
［54］Lv DK, Bai X, Li Y, et al. Profiling of cold-stress-responsive mi-RNAs in rice by microarrays［J］. Gene, 2010, 459（1-2）：39-47.
［55］Gao P, Bai X, Yang L, et al. Over-expression of osa-MIR396c decr-eases salt and alkali stress tolerance［J］. Planta, 2010, 231（5）：991-1001.
［56］Bottino MC, Rosario S, Grativol C, et al. High-throughput sequencing of small RNA transcriptome reveals salt stress regulated microRNAs in sugarcane［J］. PLoS One, 2013, 8（3）：e59423.
［57］Navarro L, Dunoyer P, Jay F, et al. A plant miRNA contributes to antibacterial resistance by repressing auxin signaling［J］. Science, 2006, 312（5772）：436-439.
［58］Fahlgren N, Howell MD, Kasschau KD, et al. High-throughput sequencing of Arabidopsis microRNAs：evidence for frequent birth and death of MIRNA genes［J］. PLoS One, 2007, 2（2）：e219.
［59］Ito H. Small RNAs and regulation of transposons in plants［J］. Genes Genet Syst, 2013, 88（1）：3-7.
［60］Yao YL, Bilichak A, Golubov A, et al. Differential sensitivity of Arabidopsis siRNA biogenesis mutants to genotoxic stress［J］. Plant Cell Rep, 2010, 29（12）：1401-1410.
［61］Katiyar-Agarwal S, Morgan R, Dahlbeck D, et al. A pathogen-inducible endogenous siRNA in plant immunity［J］. Proc Natl Acad Sci USA, 2006, 103（47）：18002-18007.
［62］Sahu PP, Rai NK, Chakraborty S, et al. Tomato cultivar tolerant to Tomato leaf curl New Delhi virus infection induces virus-specific short interfering RNA accumulation and defence-associated host gene expression［J］. Mol Plant Pathol, 2010, 11（14）：531-544.
［63］Sahu PP, Rai NK, Puranik S, et al. Dynamics of defense-related components in two contrasting genotypes of tomato upon infection with tomato leaf curl new Delhi virus［J］. Mol Biotechnol, 2012, 52（2）：140-150.

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