利用分子生物学技术提高微生物有机溶剂耐受性的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.015

摘要/Abstract

摘要： 耐有机溶剂微生物是一类能够在较高浓度有机溶剂中存活或者生长的微生物, 其在非水相生物催化等领域表现出巨大的应用优势。与自然筛选、长期驯化和传统诱变等方法相比, 利用分子生物学技术获得微生物有机溶剂耐受菌株是一种更为理性且高效的手段。主要综述了近年来利用分子生物学技术对耐性相关功能基因和转录因子进行改造, 提高微生物有机溶剂耐受性的研究进展, 并展望了有机溶剂耐受菌株的应用前景。

关键词: 有机溶剂耐受性, 分子生物学技术, 工程菌, 生物转化

Abstract: Organic-solvent-tolerant microorganisms can survive and grow in the higher concentrations of organic solvents, which exhibit significant application advantages in the field of the non-aqueous phase biocatalysis. Compared to natural screening, long-term domestication and traditional mutagenesis methods, molecular technique is a more rational and efficient way to obtain organic-solvent-tolerant strains. This paper mainly summarizes the research advances on the improvement of microbial organic-solvent tolerance by the reformation of functional genes related to the resistance to organic solvents and transcriptional factors using molecular biology techniques, also prospects the application of organic-solvent-tolerant strain.

Key words: organic solvent tolerance, molecular biology techniques, engineered strain, biotransformation

王艳霞,刘祥胜,王敏,骆健美. 利用分子生物学技术提高微生物有机溶剂耐受性的研究进展[J]. 生物技术通报, 2015, 31(10): 77-88.

Wang Yanxia, Liu Xiangsheng, Wang Min, Luo Jianmei. Research Advances on Molecular Techniques for Improving Microbial Tolerance to Organic Solvents[J]. Biotechnology Bulletin, 2015, 31(10): 77-88.

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