生物技术通报 ›› 2016, Vol. 32 ›› Issue (12): 34-39.

• 技术与方法 • 上一篇    下一篇

植物糖基转移酶基因的分离方法及其生物学功能研究进展

罗燕,刘小刚,周志钦   

  1. 西南大学园艺园林学院,重庆 400716
  • 收稿日期:2016-02-26 出版日期:2016-12-25 发布日期:2016-12-07
  • 作者简介:罗燕,女,硕士,研究方向:柑橘类黄酮生物合成代谢;E-mail:wodeaily7@163.com
  • 基金资助:
    基于DNA条形码和基因芯片技术的柑橘物种鉴定与分类研究(31171930)

Research Progress on Methods for Isolating the Gene of Plant Glycosyltransferase,and Its Biological Functions

LUO Yan,LIU Xiao-gang,ZHOU Zhi-qin   

  1. School of Horticulture and Landscape,Southwest University,Chongqing 400716
  • Received:2016-02-26 Published:2016-12-25 Online:2016-12-07

摘要: 植物糖基转移酶是植物体内广泛存在的一种进行糖基化反应的转移酶,可以对糖、蛋白质等受体化合物进行糖基化修饰,从而改变其理化性质,对植物的次生代谢和维持体内激素稳态等的生长发育以及对生物及非生物胁迫的响应具有重要的意义。综述了近几年来植物糖基转移酶研究方法及生物学功能的进展情况,并对以后的研究热点进行了展望,旨为更多植物糖基转移酶的鉴定及分离方法提供一定的借鉴,同时希望对该家族基因进一步的功能分析有所帮助。

关键词: 糖基转移酶, 转录组测序, 全基因组分析, 生物学功能, 胁迫

Abstract: Plant glycosyltransferases widely exist in plant for glycosylation reaction,and they could modify receptor chemicals,such as sugar and proteins,by glycosylation to change their physical and chemical properties,which is of significance for the growth and development of plant secondary metabolism and hormonal homeostasis maintenance,as well as responses to biotic and abiotic stresses. Here,we reviewed the research progress on the methods and biological functions of plant glycosyltransferase,and predicted the research focus of it in the future;aiming at providing some references for the identification and isolation of more plant glycosyltransferase genes,and assisting the further functional analysis of this gene family.

Key words: glycosyltransferase, transcriptome sequencing, whole genome analysis, biological function, stress

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