镉胁迫后旱柳转录组变化分析

doi:10.13560/j.cnki.biotech.bull.1985.2019-1018

摘要/Abstract

摘要： 随着重金属镉（Cd）应用范围的扩大,土壤Cd污染问题日益严重。以具有植物恢复潜力的旱柳（Saliz matsudana）作为研究对象,探究不同浓度的Cd胁迫后旱柳无性系1 d、7 d和30 d后基因表达与代谢通路的变化。转录组测序结果表明：共获得102 595个Unigenes,相同浓度不同时间的差异基因总数为26 623和32 154个;相同时间不同浓度的差异基因总数为8 550、3 444和11 428个。从中筛选得到与Cd胁迫响应密切相关的基因25个,其中金属硫蛋白、ABC转运蛋白、锌和锰转运蛋白等基因的表达不仅会随着Cd胁迫浓度变化而且同时受到胁迫时间的改变而发生改变;油菜素内酯合成通路的3,6-脱氧油菜素淄酮酶（ROT3）和黄酮类化合物合成通路的黄酮醇合成酶（FLS）、黄烷酮-3-羟化酶（F3H）均明显上调。此外旱柳响应Cd胁迫的GO条目主要集中在代谢过程、细胞过程、膜、细胞器、细胞、细胞部分、催化活化和结合蛋白上,参与这些GO条目的差异表达基因数随着Cd浓度和胁迫时间的增加而增加。通过转录组测序分析旱柳Cd胁迫后的响应机制,旨为旱柳修复土壤Cd污染提供理论指导。

关键词: 旱柳, 镉, 转录组, 差异基因

Abstract: With the expanded application of heavy metal cadmium,the soil cadmium pollution is increasingly serious. Saliz matsudana,which has potential of phytoremediation,was selected as research object,and the changes of gene expression and metabolic pathway were investigated after 1 d,7 d and 30 d under 2.5 mg/L and 50 mg/L concentrations of cadmium stress. The result of transcriptome sequencing showed that 102 595 Unigenes were obtained,and there were 26 623 and 32 154 differential expressed genes（DEGs）in the same concentration while at different stress time,and 8 550,3 444 and 11 428 DEGs in different concentrations while at the same stress time. Total 25 genes closely related to cadmium stress responses were screened from them. The changes of genes expression（such as metallothionein,ABC transporter,zinc and manganese transporter）depended on both concentration of cadmium stress and stress time. The expressions of several genes were obviously up-regulated after cadmium stress,for example,3,6-deoxyinosinone ketolase（ROT3）in brassinolide synthesis pathway,as well as flavonoid synthase（FLS）and flavanone-3-hydroxylase（F3H）in the synthesis pathway of flavonoids. In addition,GO analysis showed that GO entries were mainly enriched in metabolic processes,cellular processes,membranes,cell organelles,cells,cellular fractions,catalytic activation and binding proteins in response to cadmium stress,and the number of DGEs involved in these GO entries increased along with cadmium concentration and stress time. The analyzed response mechanisms of S. matsudana after cadmium stress in this study by transcriptome sequencing provide theoretical guidance for the remediation of cadmium pollution in soil by S. matsudana.

Key words: Saliz matsudana, cadmium, transcriptome, differential gene

曹继敏, 李双财, 何德. 镉胁迫后旱柳转录组变化分析[J]. 生物技术通报, 2020, 36(7): 32-39.

CAO Ji-min, LI Shuang-cai, HE De. Transcriptome Analysis of Saliz matsudana Under Cadmium Stress[J]. Biotechnology Bulletin, 2020, 36(7): 32-39.

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