生物技术通报 ›› 2023, Vol. 39 ›› Issue (10): 231-245.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0315

• 研究报告 • 上一篇    下一篇

菊花根状茎发育的转录组分析

徐俊1(), 叶雨晴1, 牛雅静2, 黄河1(), 张蒙蒙2()   

  1. 1.北京林业大学园林学院 花卉种质资源创新与分子育种北京市重点实验室 国家花卉工程技术研究中心 城乡生态环境北京实验室,北京 100083
    2.北京市植物园管理处 北京市花卉园艺工程技术研究中心,北京 100093
  • 收稿日期:2023-04-08 出版日期:2023-10-26 发布日期:2023-11-28
  • 通讯作者: 黄河,男,博士,教授,研究方向:花卉分子生物学;E-mail: 101navy@163.com
  • 作者简介:徐俊,男,硕士研究生,研究方向:花卉种质资源与遗传育种;E-mail: 1405549321@qq.com
  • 基金资助:
    北京市植物园管理处科技课题(BZ202203)

Transcriptome Analysis of Rhizome Development in Chrysanthemum× × morifolium

XU Jun1(), YE Yu-qing1, NIU Ya-jing2, HUANG He1(), ZHANG Meng-meng2()   

  1. 1. School of Landscape Architecture, Beijing Forestry University, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 100083
    2. Beijing Botanical Garden, Beijing Floriculture Engineering Technology Research Centre, Beijing 100093
  • Received:2023-04-08 Published:2023-10-26 Online:2023-11-28

摘要:

为了探究菊花根状茎形成的分子机制,本研究选取了具有稳定根状茎的菊花株系‘2017XS’的根状茎尖、根状茎中部、根状茎下部、叶片、茎段、根系、茎尖、舌状花8 个部位进行了转录组测序,并利用生物信息学方法对根状茎尖和整个根状茎中高表达的基因进行筛选。转录组测序共得到159.51 GB 数据,组装后得到100 235个Unigene。其中,64 956(64.80%)个Unigene在7个公共蛋白质数据库中得到了注释。为了找到根状茎发育的关键基因,通过加权基因共表达网络分析(weighted correlation network analysis,WGCNA)、K-means和基于Venn筛选差异基因3种方式筛选在根状茎尖和根状茎中高表达基因。最终筛选到20个在根状茎尖中高表达的基因和36个在根状茎中高表达的基因。这些基因包括了和植物非生物胁迫相关的基因、脱落酸(abscisic acid, ABA)代谢基因、红光受体和紫外光受体以及光周期核心转录因子,选取6个差异表达较显著的基因进行 RT-qPCR 荧光定量分析,结果与转录组测序数据一致,验证了转录组的有效性。且这些差异表达基因在同样具根茎的菊花株系‘2005042’中亦表现为在根状茎中特异高表达。综上,菊花根状茎的形成和发育可能受到包括脱落酸在内的植物激素以及光周期等因素的影响,本研究对进一步探究菊花根状茎发育的分子机制提供了重要依据。

关键词: 根状茎, 转录组, 内源激素

Abstract:

In order to explore the molecular mechanism of chrysanthemum rhizome formation, we performed transcriptome analysis of eight tissues(RH: rhizome apical; RT: middle of rhizome; RB: bottle of rhizome; SA: shoot apical; L: leaf; S: shoot; R: root; F: ray floret)of chrysanthemum. Approximately 159.51 gigabase(GB)paired-end clean reads were obtained and assembled into 100 235 Unigenes. Among these Unigenes, 64 956(64.80%)were annotated in seven public protein databases. In order to find the key genes of rhizome development, the highly expressed genes in the rhizome tip and rhizome were screened by weighted correlation network analysis(WGCNA), K-means and screening differential genes based on Venn. Finally, a total of 20 highly expressed genes in the rhizome tip and 36 highly expressed genes in the rhizome were obtained. These genes include a large number of genes related to plant abiotic stress, the ABA metabolic gene. In addition, the phytochrome gene PHYB, the UV-B photoreception gene UVR8, and the photoperiod core transcription factors were also uncovered, six differentially expressed genes were selected for RT-qPCR analysis, and the results were consistent with RNA-seq data, verifying the validity of RNA-seq data. And these differentially expressed genes were also expressed differentially in the rhizomes of chrysanthemum strain‘2005042’.Taken above together, the formation and development of chrysanthemum rhizomes may be affected by plant hormones including abscisic acid and photoperiod. This study provides important clues to further explore the molecular mechanism of the development of chrysanthemum rhizomes.

Key words: rhizome, transcriptome, endogenous hormones