环境胁迫下内生菌与宿主代谢相互作用研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-0973

摘要/Abstract

摘要：

植物体内成分是实时反映其生理状态的最直接指标,是其遭受生物或非生物胁迫应激状态的体现,微生物与植物的共生抗逆亦由代谢的重置与调控得以实现。内生菌可以自身细胞功能或代谢产物调控宿主代谢,其自身可产生独特的、显著区别于宿主的代谢成分参与抗逆;而宿主内环境的长期“驯化”亦可改变内生菌的表型和代谢。较全面地分析了植物与微生物共生抗逆在代谢层面的相互作用,旨为同一领域工作者提供有价值的参考。

关键词: 代谢调控, 植物与内生菌互作, 共生抗逆, 代谢变化

Abstract:

Chemicals inside plants are the direct indicators of their physiological status under biotic or abiotic stresses in real time. The symbiotic resistance of microorganisms and plants is also achieved by the reset and regulation of metabolism. Endophytes can regulate host metabolism by their own cell functions or metabolites,and they can produce unique and significantly different metabolic components from the host to participate in stress resistance. The long-term“domestication” of the host environment can also change the phenotype and metabolism of endophytes. This review comprehensively analyzed the interactions between plants and microorganism on symbiotic resistance at metabolic level,and may provide valuable reference for colleagues working in the same field.

Key words: metabolic regulation, interaction between plants and endophytes, symbiotic resistance, metabolic variation

马勤, 雷瑞峰, 迪力热巴·阿不都肉苏力, 穆耶赛尔·奥斯曼, 祖力胡玛尔·肉孜, 安登第. 环境胁迫下内生菌与宿主代谢相互作用研究进展[J]. 生物技术通报, 2021, 37(3): 153-161.

MA qin, LEI Rui-feng, Dilireba Abudourousuli, Muyesaier Aosiman, Zulihumaer Rouzi, AN Deng-di. Research Progress on the Symbiotic Metabolic of Endophytes and Plants Under Stress[J]. Biotechnology Bulletin, 2021, 37(3): 153-161.

图/表 1

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菌株	宿主植物	调控模式	主要基因/成分	参考文献
Streptomyces sp. EN27	拟南芥 Arabidopsis thaliana	激活系统获得性抗性（SAR）	诱导PR-1基因	[33]
Streptomyces sp. EN28	拟南芥 Arabidopsis thaliana	触发茉莉酸/乙烯（JA/ET）防卫信号途径	诱导PDF1.2基因	[33]
Bacillus thuringiensis GS1	黄瓜 Cucumis sativus L.	诱导病程相关蛋白和酶,引起防御反应	诱导PR蛋白、愈疮木酚过氧化物酶（GPOD）、多酚氧化酶（PPO）	[34]
Pseudomonasfluorescens SS101	拟南芥 Arabidopsis thaliana	促进植物生长	释放13-Tetradecadien-1-ol,2-butanone 和2-甲基-n-1-十三碳烯等挥发性有机物	[35]
Pseudomonas fluorescens FPT9601-T5	拟南芥 Arabidopsis thaliana	激活系统抗性	升高内源性SA水平、上调PR蛋白的表达和SAR相关基因（例如咖啡酰辅酶A 3-O-甲基转移酶）	[36]
Piriformospora indica	大麦Hordeum vulgare L.	糖、氮代谢和激素生物合成	海藻糖-6-磷酸合酶（TPS）、海藻糖磷酸磷酸酯酶（TPP）的表达升高;谷氨酰胺合成酶1（GS1）下调,谷氨酰胺合成酶2（GS2）上调;ABA基因HV22D表达下调,正调控ACC氧化酶	[37]
Pseudomonas putida 1290	萝卜Raphanus sativus L.	植物激素	升高加氧酶降解IAA	[44]
Bacillus licheniformis SA03	菊花 Chrysanthemum sp.	激活非生物胁迫和铁获取相关途径	通过ABA和NO激活适应性反应	[48]
Pseudonocardia sp.	黄花蒿 Artemisia annua L.	诱导次生代谢	诱导紫穗槐-4,11-二烯合酶（ADS）、细胞色素P450羟化酶（CYP71AV1）、细胞色素P450氧化还原酶（CPR）的调控基因,提高青蒿素的产量	[49]
Clavicipitilean endophytes	黑麦草 Lolium perenne	诱导宿主的次生代谢	诱导具有抗氧化活性的绿原酸的合成	[43]
Taxomyces andreanae	短叶红豆杉 Taxus brevifolia	与宿主共享代谢	持续产生紫杉醇	[53]
Trichoderma	喜树 Camptotheca acuminata	与宿主共享代谢	持续产生喜树碱	[57]
Diaporthe sp.	山茶树 Oiltea camellia	转化宿主代谢物	C4 -黄烷选择性反应生成二氢黄烷衍生物	[59]
B. subtilis Roh-1	大豆 Glycine max	转化宿主代谢产物	将大豆苷元转化为正二羟基异黄酮和大豆黄素	[63]
P. variabile	柱冠粗榧 Cephalotaxus harringtonia	转化宿主代谢产物	通过脱糖作用将糖基化黄酮类化合物转化为相应苷元	[65]

菌株	宿主植物	调控模式	主要基因/成分	参考文献
Streptomyces sp. EN27	拟南芥 Arabidopsis thaliana	激活系统获得性抗性（SAR）	诱导PR-1基因	[33]
Streptomyces sp. EN28	拟南芥 Arabidopsis thaliana	触发茉莉酸/乙烯（JA/ET）防卫信号途径	诱导PDF1.2基因	[33]
Bacillus thuringiensis GS1	黄瓜 Cucumis sativus L.	诱导病程相关蛋白和酶,引起防御反应	诱导PR蛋白、愈疮木酚过氧化物酶（GPOD）、多酚氧化酶（PPO）	[34]
Pseudomonasfluorescens SS101	拟南芥 Arabidopsis thaliana	促进植物生长	释放13-Tetradecadien-1-ol,2-butanone 和2-甲基-n-1-十三碳烯等挥发性有机物	[35]
Pseudomonas fluorescens FPT9601-T5	拟南芥 Arabidopsis thaliana	激活系统抗性	升高内源性SA水平、上调PR蛋白的表达和SAR相关基因（例如咖啡酰辅酶A 3-O-甲基转移酶）	[36]
Piriformospora indica	大麦Hordeum vulgare L.	糖、氮代谢和激素生物合成	海藻糖-6-磷酸合酶（TPS）、海藻糖磷酸磷酸酯酶（TPP）的表达升高;谷氨酰胺合成酶1（GS1）下调,谷氨酰胺合成酶2（GS2）上调;ABA基因HV22D表达下调,正调控ACC氧化酶	[37]
Pseudomonas putida 1290	萝卜Raphanus sativus L.	植物激素	升高加氧酶降解IAA	[44]
Bacillus licheniformis SA03	菊花 Chrysanthemum sp.	激活非生物胁迫和铁获取相关途径	通过ABA和NO激活适应性反应	[48]
Pseudonocardia sp.	黄花蒿 Artemisia annua L.	诱导次生代谢	诱导紫穗槐-4,11-二烯合酶（ADS）、细胞色素P450羟化酶（CYP71AV1）、细胞色素P450氧化还原酶（CPR）的调控基因,提高青蒿素的产量	[49]
Clavicipitilean endophytes	黑麦草 Lolium perenne	诱导宿主的次生代谢	诱导具有抗氧化活性的绿原酸的合成	[43]
Taxomyces andreanae	短叶红豆杉 Taxus brevifolia	与宿主共享代谢	持续产生紫杉醇	[53]
Trichoderma	喜树 Camptotheca acuminata	与宿主共享代谢	持续产生喜树碱	[57]
Diaporthe sp.	山茶树 Oiltea camellia	转化宿主代谢物	C4 -黄烷选择性反应生成二氢黄烷衍生物	[59]
B. subtilis Roh-1	大豆 Glycine max	转化宿主代谢产物	将大豆苷元转化为正二羟基异黄酮和大豆黄素	[63]
P. variabile	柱冠粗榧 Cephalotaxus harringtonia	转化宿主代谢产物	通过脱糖作用将糖基化黄酮类化合物转化为相应苷元	[65]