生物技术通报 ›› 2022, Vol. 38 ›› Issue (1): 98-107.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0132
收稿日期:
2021-02-02
出版日期:
2022-01-26
发布日期:
2022-02-22
作者简介:
杨馥榕,硕士研究生,研究方向:园林植物与应用;E-mail: 基金资助:
YANG Fu-rong(), WANG Xiao-hong(), XIAO Qi, FANG Juan, LI Li-hua
Received:
2021-02-02
Published:
2022-01-26
Online:
2022-02-22
摘要:
研究镉胁迫对木槿的生理影响,比较木槿品种的镉耐受能力,为镉污染地区的植物材料选择提供依据。以3个木槿品种为材料,采取盆栽控制试验,测定其生长、生理指标,通过隶属函数分析综合比较品种耐镉能力。木槿的株高与地径增长量、叶绿素A+B与可溶性蛋白含量随胁迫浓度增加呈先上升后降低或逐渐降低趋势,而且随时间延长,降低程度不断加强;游离脯氨酸、丙二醛含量、SOD活性随胁迫浓度与胁迫时间增加而逐渐上升,但长时间高浓度胁迫下,不同品种会出现不同程度的下降;隶属函数分析发现品种耐受能力因胁迫浓度有所差异。镉胁迫浓度对木槿生长有低促高抑作用,而且随时间延长出现促进减弱、抑制加强;3个品种中‘红星’的表现最佳,值得推广。
杨馥榕, 王晓红, 肖琪, 方娟, 李立华. 木槿品种对镉胁迫的生理响应及耐镉能力评价[J]. 生物技术通报, 2022, 38(1): 98-107.
YANG Fu-rong, WANG Xiao-hong, XIAO Qi, FANG Juan, LI Li-hua. Physiological Response of Hibiscus syriacus Varieties to Cadmium Stress and Evaluation of Cadmium Tolerance[J]. Biotechnology Bulletin, 2022, 38(1): 98-107.
品种 Varieties | 镉处理浓度 Cadmium treatment concentration/(mg·kg-1) | 株高增长量 Growth of plant height/cm | 地径增长量 Growth of the earth’s path/cm |
---|---|---|---|
‘牡丹’ ‘f. paeoniflorus’ | 0 | 35.633±23.981a | 0.226±0.212a |
50 | 18.267±3.19ab | 0.316±0.087a | |
100 | 20.667±12.254ab | 0.236±0.109a | |
200 | 7.667±1.704b | 0.131±0.087a | |
400 | 5.6±1.803b | 0.101±0.052a | |
‘红星’ ‘Hong xing’ | 0 | 17.833±5.829ab | 0.224±0.137a |
50 | 33.533±14.994a | 0.221±0.114a | |
100 | 21.567±13.428ab | 0.203±0.063a | |
200 | 22.8±3.951ab | 0.161±0.077a | |
400 | 6.667±3.63b | 0.101±0.096a | |
‘白花重瓣’ ‘f. albus-plenus’ | 0 | 13.067±9.028a | 0.26±0.158a |
50 | 22.4±14.245a | 0.217±0.151a | |
100 | 10.2±1.97a | 0.12±0.048a | |
200 | 8.033±6.463a | 0.097±0.044a | |
400 | 7.2±5.012a | 0.094±0.019a |
表1 不同镉浓度处理60 d后3个木槿品种生长指标变化情况
Table 1 Changes of growth indexes of three Hibiscus syriacus varieties treated with different cadmium concentrations for 60 d
品种 Varieties | 镉处理浓度 Cadmium treatment concentration/(mg·kg-1) | 株高增长量 Growth of plant height/cm | 地径增长量 Growth of the earth’s path/cm |
---|---|---|---|
‘牡丹’ ‘f. paeoniflorus’ | 0 | 35.633±23.981a | 0.226±0.212a |
50 | 18.267±3.19ab | 0.316±0.087a | |
100 | 20.667±12.254ab | 0.236±0.109a | |
200 | 7.667±1.704b | 0.131±0.087a | |
400 | 5.6±1.803b | 0.101±0.052a | |
‘红星’ ‘Hong xing’ | 0 | 17.833±5.829ab | 0.224±0.137a |
50 | 33.533±14.994a | 0.221±0.114a | |
100 | 21.567±13.428ab | 0.203±0.063a | |
200 | 22.8±3.951ab | 0.161±0.077a | |
400 | 6.667±3.63b | 0.101±0.096a | |
‘白花重瓣’ ‘f. albus-plenus’ | 0 | 13.067±9.028a | 0.26±0.158a |
50 | 22.4±14.245a | 0.217±0.151a | |
100 | 10.2±1.97a | 0.12±0.048a | |
200 | 8.033±6.463a | 0.097±0.044a | |
400 | 7.2±5.012a | 0.094±0.019a |
图1 镉胁迫下3个木槿品种叶绿素A+B含量变化情况 不同小写字母表示差异显著(P <0.05),下同
Fig. 1 Changes in chlorophyll A+B content of three H. syriacus varieties under cadmium stress Different lowercase letters indicate significant difference(P < 0.05),the same below
Cd处理浓度Cadmium concentration/(mg·kg-1) | 品种 Varieties | 隶属函数值 Membership function value | 平均值 Average value | 排名 Ranking | ||||||
---|---|---|---|---|---|---|---|---|---|---|
X1 | X2 | X3 | X4 | X5 | X6 | X7 | ||||
50 | ‘牡丹’(‘f. paeoniflorus’) | 0.368 | 0.417 | 0.392 | 0.539 | 0.556 | 0.367 | 0.481 | 0.446 | 3 |
‘红星’(‘Hong xing’) | 0.532 | 0.634 | 0.461 | 0.642 | 0.500 | 0.371 | 0.409 | 0.507 | 1 | |
‘白花重瓣’(‘f. albus-plenus’) | 0.578 | 0.353 | 0.560 | 0.355 | 0.667 | 0.422 | 0.518 | 0.493 | 2 | |
100 | ‘牡丹’(‘f. paeoniflorus’) | 0.560 | 0.454 | 0.557 | 0.475 | 0.667 | 0.410 | 0.520 | 0.520 | 3 |
‘红星’(‘Hong xing’) | 0.460 | 0.559 | 0.646 | 0.646 | 0.567 | 0.639 | 0.529 | 0.578 | 1 | |
‘白花重瓣’(‘f. albus-plenus’) | 0.421 | 0.403 | 0.637 | 0.477 | 0.630 | 0.599 | 0.515 | 0.526 | 2 | |
200 | ‘牡丹’(‘f. paeoniflorus’) | 0.480 | 0.479 | 0.420 | 0.381 | 0.500 | 0.600 | 0.426 | 0.469 | 2 |
‘红星’(‘Hong xing’) | 0.608 | 0.544 | 0.603 | 0.658 | 0.400 | 0.465 | 0.467 | 0.535 | 1 | |
‘白花重瓣’(‘f. albus-plenus’) | 0.399 | 0.462 | 0.427 | 0.400 | 0.452 | 0.399 | 0.537 | 0.439 | 3 | |
400 | ‘牡丹’(‘f. paeoniflorus’) | 0.429 | 0.427 | 0.384 | 0.548 | 0.524 | 0.353 | 0.521 | 0.455 | 3 |
‘红星’(‘Hong xing’) | 0.463 | 0.560 | 0.337 | 0.394 | 0.422 | 0.495 | 0.602 | 0.468 | 2 | |
‘白花重瓣’(‘f. albus-plenus)’ | 0.480 | 0.526 | 0.558 | 0.502 | 0.377 | 0.592 | 0.500 | 0.505 | 1 |
表2 3个木槿品种耐镉能力综合评价
Table 2 Comprehensive evaluation of Cd resistance of three H. syriacus varieties
Cd处理浓度Cadmium concentration/(mg·kg-1) | 品种 Varieties | 隶属函数值 Membership function value | 平均值 Average value | 排名 Ranking | ||||||
---|---|---|---|---|---|---|---|---|---|---|
X1 | X2 | X3 | X4 | X5 | X6 | X7 | ||||
50 | ‘牡丹’(‘f. paeoniflorus’) | 0.368 | 0.417 | 0.392 | 0.539 | 0.556 | 0.367 | 0.481 | 0.446 | 3 |
‘红星’(‘Hong xing’) | 0.532 | 0.634 | 0.461 | 0.642 | 0.500 | 0.371 | 0.409 | 0.507 | 1 | |
‘白花重瓣’(‘f. albus-plenus’) | 0.578 | 0.353 | 0.560 | 0.355 | 0.667 | 0.422 | 0.518 | 0.493 | 2 | |
100 | ‘牡丹’(‘f. paeoniflorus’) | 0.560 | 0.454 | 0.557 | 0.475 | 0.667 | 0.410 | 0.520 | 0.520 | 3 |
‘红星’(‘Hong xing’) | 0.460 | 0.559 | 0.646 | 0.646 | 0.567 | 0.639 | 0.529 | 0.578 | 1 | |
‘白花重瓣’(‘f. albus-plenus’) | 0.421 | 0.403 | 0.637 | 0.477 | 0.630 | 0.599 | 0.515 | 0.526 | 2 | |
200 | ‘牡丹’(‘f. paeoniflorus’) | 0.480 | 0.479 | 0.420 | 0.381 | 0.500 | 0.600 | 0.426 | 0.469 | 2 |
‘红星’(‘Hong xing’) | 0.608 | 0.544 | 0.603 | 0.658 | 0.400 | 0.465 | 0.467 | 0.535 | 1 | |
‘白花重瓣’(‘f. albus-plenus’) | 0.399 | 0.462 | 0.427 | 0.400 | 0.452 | 0.399 | 0.537 | 0.439 | 3 | |
400 | ‘牡丹’(‘f. paeoniflorus’) | 0.429 | 0.427 | 0.384 | 0.548 | 0.524 | 0.353 | 0.521 | 0.455 | 3 |
‘红星’(‘Hong xing’) | 0.463 | 0.560 | 0.337 | 0.394 | 0.422 | 0.495 | 0.602 | 0.468 | 2 | |
‘白花重瓣’(‘f. albus-plenus)’ | 0.480 | 0.526 | 0.558 | 0.502 | 0.377 | 0.592 | 0.500 | 0.505 | 1 |
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