生物技术通报 ›› 2022, Vol. 38 ›› Issue (2): 10-20.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0542

• 研究报告 • 上一篇    下一篇

拟南芥RPP1A参与幼苗生长的蛋白质组学分析

李兵娟1,2(), 郑璐1, 沈仁芳1,2, 兰平1,2()   

  1. 1.中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京 210008
    2.中国科学院大学,北京 100049
  • 收稿日期:2021-04-23 出版日期:2022-02-26 发布日期:2022-03-09
  • 作者简介:李兵娟,女,博士研究生,研究方向:植物营养蛋白质组学;E-mail: libingjuan313@163.com
  • 基金资助:
    国家重点研发计划(2016YFD0200308);国家自然科学基金项目(32070279);国家自然科学基金项目(31370280)

Proteomic Analysis of RPP1A Involved in the Seedling Growth of Arabidopsis thaliana

LI Bing-juan1,2(), ZHENG Lu1, SHEN Ren-fang1,2, LAN Ping1,2()   

  1. 1. State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008
    2. University of Chinese Academy of Sciences,Beijing 100049
  • Received:2021-04-23 Published:2022-02-26 Online:2022-03-09

摘要:

核糖体蛋白不仅参与蛋白质合成,而且参与植物生长发育的调控。利用拟南芥核糖体磷酸蛋白P1(ribosomal phosphoprotein P1,RPP1)家族基因RPP1A缺失突变体rpp1a研究RPP1A缺失对幼苗蛋白质表达水平的影响,揭示其参与调控幼苗生长的作用机制。表型分析发现,与野生型WT相比,RPP1A缺失导致拟南芥幼苗生物量、叶片总表面积和叶柄长度显著增加。基于label-free的蛋白质组学分析,与野生型相比,rpp1a-2突变体有280个蛋白质表达量具有显著差异,其中98个显著上调,182个显著下调。差异蛋白主要富集在细胞过程、代谢过程、刺激反应、细胞成分组织或生物发生和氧化还原过程等生物学过程,并在核糖体、光合作用、mRNA监视途径、RNA降解和苯丙素的生物合成代谢通路中显著富集。拟南芥rpp1a-2突变体通过抑制刺激反应和增强光合作用等过程促进植物生长。

关键词: 拟南芥, 核糖体蛋白, 幼苗生长, 蛋白质组, 刺激反应, 光合作用

Abstract:

Ribosomal proteins are not only involved in protein synthesis but also regulate plant growth and development. Arabidopsis thaliana ribosomal phosphoprotein P1(RPP1)family RPP1A deletion mutant rpp1a was used to investigate the effect of RPP1A deletion on the protein profile of seedlings and to reveal the mechanism of its involvement in regulation of seedling growth. Phenotypic analysis revealed that the RPP1A deletion resulted in significant increases in biomass,total leaf surface area,and petiole length of the seedlings compared with wild-type(WT). The label-free proteomic analysis revealed that a total of 280 differentially expressed proteins were identified in the mutant rpp1a-2 compared with the WT,with 98 proteins significantly up-regulated and 182 proteins significantly down-regulated. Differentially expressed proteins were mainly involved in cellular process,metabolic process,response to stimulus,cellular component organization or biogenesis,and oxidation-reduction process,and were significantly enriched in the metabolic pathways of ribosome,photosynthesis,mRNA surveillance pathway,RNA degradation and phenylpropanoid biosynthesis. The A. thaliana rpp1a-2 mutant promotes plant growth by inhibiting the stimulus-response and enhancing photosynthesis.

Key words: Arabidopsis thaliana, ribosomal proteins, seedling growth, proteomics, stimulus response, photosynthesis