生物技术通报 ›› 2022, Vol. 38 ›› Issue (2): 245-251.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0975

• 综述与专论 • 上一篇    下一篇

核糖核酸5'端NAD+帽子修饰研究进展

董海娇1,2(), 杨晓玉3, 莫蓓莘1, 陈雪梅4, 崔洁1()   

  1. 1.深圳大学生命与海洋科学学院,深圳 518037
    2.深圳大学光电工程学院 光电子器件与系统(教育部/广东省)重点实验室,深圳 518060
    3.山东农业大学园艺科学与工程学院,泰安 271018
    4.美国加利福尼亚大学河滨分校整合基因组学研究中心/植物科学系,美国加州河滨 92521
  • 收稿日期:2021-07-30 出版日期:2022-02-26 发布日期:2022-03-09
  • 作者简介:董海娇,女,博士,研究方向:植物表观遗传学;E-mail: 547860420@qq.com
  • 基金资助:
    国家自然科学基金青年科学基金项目(31801077);国家自然科学基金青年科学基金项目(32000153);中国博士后科学基金面上资助(2018M640822);深圳市高等院校稳定支持计划面上项目(20200809224536001)

Research Progress in NAD+ Cap Modification at the 5' End of RNA

DONG Hai-jiao1,2(), YANG Xiao-yu3, MO Bei-xin1, CHEN Xue-mei4, CUI Jie1()   

  1. 1. College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518037
    2. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University,Shenzhen 518060
    3. College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018
    4. Department of Botany and Plant Sciences,Institute of Integrative Genome Biology,University of California,Riverside,CA,92521,USA
  • Received:2021-07-30 Published:2022-02-26 Online:2022-03-09

摘要:

m7G帽子具有保护RNA不被降解以及招募相关蛋白参与内含子剪切、poly(A)加尾、出核和翻译等功能。一直以来,它被认为是真核生物mRNA所特有的修饰类型。然而近年来,在包括原核生物在内的多个物种中均检测到一种新的RNA 5'端修饰,即核酸代谢物烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NAD+)帽子。目前NAD+修饰RNA(NAD-RNA)的生物学功能研究仍处于起始阶段。本文概述了NAD-RNA的发现及其检测和鉴定技术的发展;探讨了NAD+帽子对RNA的调控功能,以及NAD-RNA脱帽和加帽的影响因素;并进一步推测NAD-RNA在生物的生长、发育和环境响应中发挥的潜在功能。最后,展望了未来NAD-RNA的研究方向和主题。

关键词: RNA帽子, NAD+, NAD-RNA, 转录后调控, 生物学功能

Abstract:

The m7G cap,which has long been considered as a hallmark of eukaryotic mRNAs,protects RNAs from degradation and recruits the machinery for splicing,polyadenylation,nuclear export,and translation. The nucleotide-containing metabolite,nicotinamide adenine dinucleotide(NAD+),has recently been detected at the 5' end of RNAs in several species including prokaryotes as a new cap. Now research on the biological functions of NAD+-capped RNAs(NAD-RNAs)is still at the initial stage. This review summarizes the discovery of NAD-RNAs as well as the development of its detection and identification technology,discusses the regulatory functions of the NAD+ cap on RNA as well as the factors affecting RNA NAD+ capping and decapping,and speculates on the potential functions of NAD-RNAs in growth,development and environmental responses. Finally,we propose future research directions and topics in order to garner attention on the outstanding questions regarding the molecular and biological functions NAD-RNAs.

Key words: RNA cap, NAD+, NAD-RNA, post-transcriptional regulation, biological function