生物技术通报 ›› 2022, Vol. 38 ›› Issue (7): 178-185.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1117

• 研究报告 • 上一篇    下一篇

甜瓜CmABCG8基因的表达特性分析

洪天澍1(), 海英2, 恩和巴雅尔1, 高峰1()   

  1. 1.内蒙古师范大学生命科学与技术学院,呼和浩特 010022
    2.内蒙古兴安盟科尔沁右翼前旗第一中学,兴安盟 137400
  • 收稿日期:2021-08-30 出版日期:2022-07-26 发布日期:2022-08-09
  • 作者简介:洪天澍,男,硕士研究生,研究方向:生物化学与分子生物学;E-mail: hongtianshuu@163.com
  • 基金资助:
    国家自然科学基金项目(31660577);内蒙古自治区高等学校青年科技英才支持计划(NJYT-19-B19);内蒙古自然科学基金项目(2020MS03013)

Analysis of Expression Characteristics of CmABCG8 Gene in Cucumis melo L.

HONG Tian-shu1(), HAI Ying2, ENHE Ba-ya-er1, GAO Feng1()   

  1. 1. College of Life Science and Technology,Inner Mongolia Normal University,Hohhot 010022
    2. First Middle School of Horqin Right Front Banner,Hinggan League,Inner Mongolia,Hinggan League 137400
  • Received:2021-08-30 Published:2022-07-26 Online:2022-08-09

摘要:

ABC转运蛋白,是一类功能广泛的具有主动介导多种分子转运的蛋白质,但在甜瓜(Cucumis melo L.)中的研究尚不清楚。本文通过生物信息学方法分析了ABC转运蛋白亚家族G成员CmABCG8的特点,同时使用荧光定量PCR检测了CmABCG8的不同时期果实、不同组织表达特性及对逆境胁迫的响应情况。进化树结果表明CmABCG8与黄瓜CsABCG23的亲缘关系最近,CmABCG8在甜瓜各组织中均有表达,在雌蕊和叶中表达量较高,CmABCG8有10个相互作用蛋白质。RT-qPCR结果表明,在果实不同时期的表达量中,CmABCG8在10 d时表达量最高;在甜瓜不同组织中,CmABCG8在叶子中表达量最高;分别用细胞分裂素、油菜素内酯、过氧化氢处理后,随着处理浓度的增加,CmABCG8的表达量均呈上升趋势;在铁、铜、锰3种金属离子处理后,CmABCG8的表达量呈下降趋势,因此分析其在甜瓜果实发育前期有一定影响,并对植物激素及金属离子有不同程度的响应,植物激素能够有效诱导CmABCG8的表达。

关键词: 甜瓜, ABC转运蛋白, 植物激素, 金属离子

Abstract:

ABC transporter is a kind of proteins with extensive functions,which can actively mediate the transport of various molecules,but its study in melon(Cucumis melo L.)is still unclear. In this paper,the characteristics of ABC transporter subfamily G member CmABCG8 were analyzed by bioinformatics method,and the expression characteristics of CmABCG8 in different fruits and tissues at different periods and their responses to stress were detected by fluorescence quantitative PCR. The phylogenetic tree results showed that CmABCG8 had the closest relationship with cucumber(Cucumis sativus L.)CsABCG23. CmABCG8 was expressed in all tissues of melon,and was highly expressed in the gynoecium and leaves. CmABCG8 had 10 interacting proteins. The results of RT-qPCR showed that the expression of CmABCG8 was the highest at 10 d. CmABCG8 expression was the highest in the leaves among different tissues of melon. After treatment with cytokinin,brassinosteroid and hydrogen peroxide,the expression of CmABCG8 presented an upward trend with the increase of the treatment concentration. After treatment with Fe2+,Cu2+ and Mn2+,the expression of CmABCG8 showed a downward trend. Therefore,it was analyzed that CmABCG8 had a certain influence on the early development of melon fruit and had different degrees of response to plant hormones and metal ions. Plant hormones could effectively induce the expression of CmABCG8.

Key words: Cucumis melo L., ABC transporter, plant hormone, metal ion