纳米硒（SeNPs）缓解烟草幼苗铅胁迫和促生效应

doi:10.13560/j.cnki.biotech.bull.1985.2024-0078

生物技术通报 ›› 2024, Vol. 40 ›› Issue (7): 183-196.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0078

纳米硒（SeNPs）缓解烟草幼苗铅胁迫和促生效应

杜仲阳¹(), 杨泽¹, 梁梦静¹, 刘义珍¹, 崔红利¹^,², 史达明¹, 薛金爱¹, 孙岩¹, 张春辉¹, 季春丽¹(), 李润植¹()

1.山西农业大学农学院分子农业与生物能源研究所山西省特用作物遗传与代谢工程研究中心，太谷 030801
2.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室，烟台 264003

收稿日期:2024-01-18 出版日期:2024-07-26 发布日期:2024-05-24
通讯作者: 李润植，男，博士，教授，研究方向：特色植物资源开发与利用；E-mail: rli2001@126.com；
季春丽，女，博士，副教授，研究方向：特色植物资源开发与利用；E-mail: jichunli@sxau.edu.cn
作者简介:杜仲阳，女，硕士研究生，研究方向：特色植物资源开发与功能验证；E-mail: zhongyang0349@163.com
基金资助:
国家重点研发计划部省联动项目(2021YFD1901100);省部共建有机旱作农业国家重点实验室（筹）自主研发课题(202105D121008-3-6);山西省高等学校科技创新计划项目(2021L119);山西省农业农村厅“六新”项目

Effect of Nano-selenium（SeNPs）in Alleviating Lead Stress and Promoting Growth of Tobacco Seedlings

DU Zhong-yang¹(), YANG Ze¹, LIANG Meng-jing¹, LIU Yi-zhen¹, CUI Hong-li¹^,², SHI Da-ming¹, XUE Jin-ai¹, SUN Yan¹, ZHANG Chun-hui¹, JI Chun-li¹(), LI Run-zhi¹()

1. College of Agronomy, Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Shanxi Engineering Research Center for Genetics and Metabolism of Special Crops, Taigu 030801
2. Key Laboratory of Coastal Biology and Bio-Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003

Received:2024-01-18 Published:2024-07-26 Online:2024-05-24

摘要/Abstract

摘要：

【目的】 旨在探究纳米硒（SeNPs）对铅（Pb）胁迫烟草幼苗的生长、抗逆性、铅吸收和转运的影响，以揭示SeNPs的促生效应、富硒和阻滞铅吸收及转运的机制。【方法】 以普通烟草（Nicotiana tabacum）为研究对象，通过盆栽实验设置不同处理，包括低剂量（100 mg/L）和高剂量（200 mg/L）Pb胁迫，无机硒（Na₂SeO₃）、SeNPs处理和空白对照组。测定各处理组烟草幼苗的生物量、光合生理参数、抗氧化酶活性、脂质过氧化产物含量以及相关基因表达水平，分析铅和硒在植物体内的含量和分布。【结果】 与对照组相比，硒处理显著促进了烟草幼苗的生长和光合作用，SeNPs促生效应更显著。硒处理还增强了烟草幼苗的抗氧化酶（SOD、POD和APX等）活性和抗氧化物质（抗坏血酸和谷胱甘肽等）的含量，降低了脂质过氧化产物（H₂O₂和MDA）的积累。在铅胁迫条件下，硒处理可显著提高烟草幼苗中硒的含量，同时降低铅的吸收和转运率。【结论】 纳米硒能显著提高植物生物量，保护光合系统，激活抗氧化系统，阻滞铅吸收和转运，促进植物硒富集和改善植物对铅胁迫的抗性。

关键词: 纳米硒, 铅胁迫, 烟草幼苗, 促生效应, 抗氧化系统, 生物有效性, 分子机制

Abstract:

【Objective】 This study is to explore effects of nano-selenium（SeNPs）on growth and stress resistance of tobacco seedlings under lead（Pb）stress, as well as Pb absorption and translocation, aiming to reveal the mechanisms of SeNPs-mediated growth promotion, Se enrichment, and inhibition of Pb uptake and transfer in plants. 【Method】 Common tobacco（Nicotiana tabacum）was selected as the test crops. Various treatments were designed and used in a series of pot experiments, including low（100 mg/L）and high（200 mg/L）doses of Pb stresses, inorganic Se（Na₂SeO₃）and SeNPs trteatments as well as blank controls. Physiological and biochemical indexes of tobacco seedlings were quantitatively measured, including biomass, photosynthetic physiological parameters, antioxidant enzyme activities, lipid peroxidation product contents, and the related gene expression levels in tobacco seedling. The contents and distribution of Se and Pb were also examined in both aboveground and underground organs. 【Result】 Compared with the control group, Se treatments significantly promoted the growth and photosynthesis of tobacco seedlings, with SeNPs showing more significant growth-promoting effect. Se treatments also enhanced the antioxidant enzyme（SOD, CAT, POD and APX）activities and accumulation of lipid peroxidation products（proline, ascorbic acid and glutathione）, followed by reducing accumulation of lipid peroxidation products（proline, ascorbic acid and glutathione）in tobacco seedlings. Under Pb stress conditions, Se treatments significantly increased the selenium content in tobacco seedlings, whereas the Pb absorption and transport rate from underground to aboveground parts were largely reduced in tobacco seedlings. 【Conclusion】 SeNPs demonstrates signicantly growth-promoting effects, including increasing plant biomass, protecting photosynthesis system, activating antioxidant system, blocking lead absorption and transport, facilitating selenium enrichment and improving plant resistance to lead stress.

Key words: selenium nanoparticles, lead stress, Nicotiana tabacum seedlings, growth-promoting effect, antioxidant mechanisms, bioavailability, molecular mechanisms

杜仲阳, 杨泽, 梁梦静, 刘义珍, 崔红利, 史达明, 薛金爱, 孙岩, 张春辉, 季春丽, 李润植. 纳米硒（SeNPs）缓解烟草幼苗铅胁迫和促生效应[J]. 生物技术通报, 2024, 40(7): 183-196.

DU Zhong-yang, YANG Ze, LIANG Meng-jing, LIU Yi-zhen, CUI Hong-li, SHI Da-ming, XUE Jin-ai, SUN Yan, ZHANG Chun-hui, JI Chun-li, LI Run-zhi. Effect of Nano-selenium（SeNPs）in Alleviating Lead Stress and Promoting Growth of Tobacco Seedlings[J]. Biotechnology Bulletin, 2024, 40(7): 183-196.

图/表 11

参考文献 51

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组别 Group	1/2 Hoagland	Se（25 mg/L）	SeNPs（25 mg/L）	Pb（100 mg/L）	Pb（200 mg/L）
con	+	-	-	-	-
se	+	+	-	-	-
SeNPs	+	-	+	-	-
pb-100	+	-	-	+	-
se-pb-100	+	+	-	+	-
SeNPs-pb-100	+	-	+	+	-
pb-200	+	-	-	-	+
se-pb-200	+	+	-	-	+
SeNPs-pb-200	+	-	+	-	+

组别 Group	1/2 Hoagland	Se（25 mg/L）	SeNPs（25 mg/L）	Pb（100 mg/L）	Pb（200 mg/L）
con	+	-	-	-	-
se	+	+	-	-	-
SeNPs	+	-	+	-	-
pb-100	+	-	-	+	-
se-pb-100	+	+	-	+	-
SeNPs-pb-100	+	-	+	+	-
pb-200	+	-	-	-	+
se-pb-200	+	+	-	-	+
SeNPs-pb-200	+	-	+	-	+

基因Gene	引物序列 Primer sequence（5'-3'）
NtPOD-F NtPOD-R	TCACTCTACCATCGCCATACTC TGCTGTTCTGCTGTCTCTTCT
NtCAT-F NtCAT-R	TCTTGCCATTGATGCCAGTTG TCCTGCCTGCTTGAAGTTGT
NtActin-F NtActin-R	CAAAGCAAGCCTACGCTCT ATACGAATGCCCCCGACT

基因Gene	引物序列 Primer sequence（5'-3'）
NtPOD-F NtPOD-R	TCACTCTACCATCGCCATACTC TGCTGTTCTGCTGTCTCTTCT
NtCAT-F NtCAT-R	TCTTGCCATTGATGCCAGTTG TCCTGCCTGCTTGAAGTTGT
NtActin-F NtActin-R	CAAAGCAAGCCTACGCTCT ATACGAATGCCCCCGACT

组别 Group	株高 Plant height/cm	根长 Root length/cm	总叶片鲜重 Total leaf fresh weight/g	总叶片干重 Total leaf dry weight/g	最大叶片鲜重 Maximum leaf fresh weight/g	最大叶片干重 Maximum leaf dry weight/g	茎秆鲜重 Stem fresh weight/g	根鲜重 Root fresh weight/g
con	26.13±0.63^a	18.46±2.99^ab	16.63±0.25^a	0.92±0.08^a	2.56±0.31^a	0.16±0.01^a	6.14±0.24^a	4.05±0.37^a
se	24.25±0.96^b	20.58±1.24^a	16.16±0.85^a	0.87±0.03^a	2.61±0.3^a	0.16±0.01^a	6.13±0.3^a	4.17±0.09^a
SeNPs	24.28±0.96^b	20.61±1.24^a	16.18±0.85^a	0.87±0.03^a	2.63±0.3^a	0.16±0.01^a	6.25±0.3^a	4.25±0.09^a
pb-100	23.38±2.29^bc	16.04±1.01^cd	14.32±0.39^cd	0.61±0.08^bc	2.53±0.49^a	0.13±0.01^b	5.45±0.49^b	2.69±0.58^bc
se-pb-100	23.88±0.25^b	17.06±1.33^bc	15.39±1.13^abc	0.68±0.02^b	2.53±0.26^a	0.13±0.01^b	5.92±0.17^a	2.95±0.28^b
SeNPs-pb-100	24.19±0.23^b	17.39±1.33^bc	15.68±1.12^ab	0.69±0.02^b	2.56±0.26^a	0.13±0.01^b	6.03±0.18^a	3±0.28^b
pb-200	21.38±1.8^d	14.56±2.14^d	13.95±0.75^d	0.54±0.01^c	2.33±0.3^a	0.12±0.01^b	5.17±0.09^b	1.82±0.61^d
se-pb-200	21.88±0.63^cd	17.64±1.15^bc	14.26±1.29^cd	0.66±0.04^b	2.39±0.31^a	0.13±0.01^b	5.21±0.43^b	2.18±0.47^cd
SeNPs-pb-200	22.14±0.64^cd	17.94±1.15^bc	14.55±1.29^bcd	0.67±0.04^b	2.42±0.31^a	0.13±0.01^b	5.31±0.43^b	2.22±0.48^cd

纳米硒（SeNPs）缓解烟草幼苗铅胁迫和促生效应

Effect of Nano-selenium（SeNPs）in Alleviating Lead Stress and Promoting Growth of Tobacco Seedlings

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Metrics

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组别 Group	硒含量 Se concentration/（μg·g^-1 FW）		铅含量 Pb concentration/（μg·g^-1 FW）
组别 Group	叶 Shoot	根 Root	叶 Shoot	根 Root
con	-	-	-	-
se	307.4±5.53^e	86.4±1.55^e	-	-
SeNPs	423.99±0.74^c	120.1±1.55^c	-	-
pb-100	-	-	88.76±1.37^d	472.02±7.29^d
se-pb-100	316.83±3.44^d	86.85±0.97^d	78.83±0.08^e	412.57±1.12^e
SeNPs-pb-100	452.3±3.39^b	127.12±0.95^b	69.97±0.91^f	375.75±4.1^f
pb-200	-	-	137.44±1.71^a	730.9±9.11^a
se-pb-200	313.76±1.69^d	89.85±0.62^d	113.76±1.69^b	612.64±4.77^b
SeNPs-pb-100	591.94±1.31^a	172.45±0.37^a	102.2±1.31^c	548.17±1.26^c

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