马铃薯LEA_2基因家族鉴定及响应低温与激素功能分析

doi:10.13560/j.cnki.biotech.bull.1985.2025-1472

生物技术通报 ›› 2026, Vol. 42 ›› Issue (6): 40-52.doi: 10.13560/j.cnki.biotech.bull.1985.2025-1472

马铃薯LEA_2基因家族鉴定及响应低温与激素功能分析

段雅馨¹(), 李成晨², 潘玉玲², 王丽², 安康², 李小波²^,³, 祝光涛¹(), 刘计涛²()

^1.云南师范大学生命科学学院马铃薯科学研究院云南省马铃薯生物学重点实验室，昆明 650500
^2.广东省农业科学院作物研究所广东省农作物遗传改良重点实验室，广州 510640
^3.广东省农业科学院蔬菜研究所，广州 510640

收稿日期:2026-01-02 出版日期:2026-06-26 发布日期:2026-07-11
通讯作者: 祝光涛，教授，研究方向：马铃薯基因组学与分子育种；E-mail: zhuguangtao@ynnu.edu.cn
刘计涛，助理研究员，研究方向：马铃薯抗逆遗传育种；E-mail: liujtpotato@126.com
作者简介:段雅馨，硕士研究生，研究方向：马铃薯抗寒分子机理；E-mail: duanyaxin399@163.com
基金资助:
国家重点研发计划项目(YS2023YFD1200005-5);广东省基础与应用基础研究重大项目(2021B0301030004);广东省基础与应用基础研究基金项目(2023A1515012611)

Identification and Functional Analysis of the LEA_2 Gene Family in Potato in Response to Cold Stress and Hormones

DUAN Ya-xin¹(), LI Cheng-chen², PAN Yu-ling², WANG Li², AN Kang², LI Xiao-bo²^,³, ZHU Guang-tao¹(), LIU Ji-tao²()

^1.College of Life Sciences, Yunnan Normal University, Potato Science Research Institute, Yunnan Key Laboratory of Potato Biology, Kunming 650500
^2.Crop Research Institute, Guangdong Academy of ?Agricultural Sciences / Guangdong Key Laboratory of Crop Genetic Improvement, Guangzhou 510640
^3.Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640

Received:2026-01-02 Published:2026-06-26 Online:2026-07-11

摘要/Abstract

摘要：

目的胚胎发育晚期丰富蛋白（late embryogenesis abundant proteins, LEA）是调节植物生长发育和逆境胁迫响应的重要基因，研究StLEA_2基因的生物学功能，为马铃薯耐寒遗传改良提供理论依据和基因资源。方法从马铃薯基因组鉴定LEA_2基因家族成员，并对其理化性质、亚细胞定位、基因结构、进化特征和顺式作用元件进行分析；采用转录组和RT-qPCR分析StLEA_2在低温胁迫、茉莉酸（JA）以及赤霉素（GA）处理下的表达模式；通过农杆菌介导的烟草瞬时过表达解析StLEA37在低温胁迫应答中的功能。结果马铃薯栽培种（Solanum tuberosum L.）鉴定出45个StLEA_2基因，耐寒野生种（Solanum commersonii）鉴定出42个ScLEA_2基因，分为9个亚族。亚细胞定位预测LEA_2家族成员主要定位于细胞质和叶绿体上；共线性分析发现马铃薯栽培种和野生种LEA家族中分别有10对和11对同源基因。StLEA_2启动子上含有大量光、逆境和激素响应元件。表达分析结果表明，44个StLEA_2基因参与低温胁迫应答；在GA与JA处理下，分别有39和43个基因表达显著变化。StLEA37定位于细胞膜上，瞬时过表达StLEA37可激活COR-CBF调节通路和活性氧清除机制参与植物低温胁迫耐性调节。结论马铃薯全基因组共鉴定得到45个StLEA_2基因。StLEA37基因定位于细胞膜上，可能通过影响COR-CBF通路、活性氧清除以及介导GA和JA信号通路参与植物低温胁迫应答。

关键词: 马铃薯, LEA_2基因家族, 低温胁迫, 茉莉酸, 赤霉素, 表达分析

Abstract:

Objective Late embryogenesis abundant proteins (LEA) are important genes that regulate plant growth and development as well as responses to abiotic stress. This study aimed to investigate the biological function of StLEA_2 genes, so as to provide a theoretical basis and genetic resources for improving potato tolerance to cold. Method Members of the LEA_2 gene family were identified from the potato genome, and their physicochemical properties, subcellular localization, gene structure, evolutionary features and cis-acting elements were analyzed. Transcriptome and RT-qPCR analyses were employed to examine the expression patterns of StLEA_2 under cold stress, jasmonic acid (JA) and gibberellic acid (GA) treatments. The function of StLEA37 in cold stress response was analyzed through Agrobacterium-mediated transient overexpression in tobacco. Result A total of 45 StLEA_2 genes were identified in cultivated potato (Solanum tuberosum L.), while 42 ScLEA_2 genes were identified in the cold-tolerant wild species (Solanum commersonii), all of them were classified into nine subgroups. Subcellular localization prediction indicates that members of the LEA_2 gene family are mainly localized in the cytoplasm and chloroplasts. Collinearity analysis revealed that there are 10 and 11 pairs of homologous genes in the LEA family of cultivated and wild potatoes, respectively. The promoters of StLEA_2 genes contain numerous light-, stress-, and hormone-responsive elements. Expression analysis indicated that 44 StLEA_2 genes are involved in responses to low-temperature stress. Under GA and JA treatments, the expressions of 39 and 43 genes, respectively, changed significantly. StLEA37 is localized on the plasma membrane, and its transient overexpression contributes to the regulation of plant cold stress tolerance by activating the COR-CBF regulatory pathway and reactive oxygen species scavenging mechanisms. Conclusion A total of 45 StLEA_2 genes were identified in the potato genome. StLEA37 is localized on the plasma membrane and may participate in plant responses to cold stress by affecting the COR-CBF pathway and ROS scavenging mechanisms, as well as by mediating GA and JA signaling pathways.

Key words: potato, LEA_2 gene family, cold stress, jasmonic acid, gibberellic acid, expression analysis

段雅馨, 李成晨, 潘玉玲, 王丽, 安康, 李小波, 祝光涛, 刘计涛. 马铃薯LEA_2基因家族鉴定及响应低温与激素功能分析[J]. 生物技术通报, 2026, 42(6): 40-52.

DUAN Ya-xin, LI Cheng-chen, PAN Yu-ling, WANG Li, AN Kang, LI Xiao-bo, ZHU Guang-tao, LIU Ji-tao. Identification and Functional Analysis of the LEA_2 Gene Family in Potato in Response to Cold Stress and Hormones[J]. Biotechnology Bulletin, 2026, 42(6): 40-52.

图/表 9

图1 马铃薯、番茄、水稻和拟南芥的LEA_2基因家族系统进化树

Fig. 1 Phylogenetic tree of the LEA_2 gene family in potato, tomato, rice and Arabidopsis

图2 马铃薯LEA_2基因家族基因结构（A）和保守氨基酸基序（B）分析

Fig. 2 Analysis of gene structure (A) and conserved amino acid motifs (B) of the LEA_2 gene family in potato

图3 马铃薯栽培种（A）与野生种（B）LEA_2基因家族共线性分析

Fig. 3 Collinearity analysis of the LEA_2 gene family in cultivated (A) and wild (B) potatoes

图4 马铃薯、番茄、拟南芥和水稻LEA_2基因家族共线性分析

Fig. 4 Collinearity analysis of the LEA_2 gene family in potato, tomato, A. thaliana, and rice

图5 马铃薯LEA_2基因家族成员启动子顺式作用调控元件

Fig. 5 Cis-acting regulatory elements in the promoters of potato LEA_2 gene family members

图6 马铃薯LEA_2基因家族低温胁迫下的表达模式分析

Fig. 6 Expression patterns analysis of the potato LEA_2 gene family under low temperature stress

图7 马铃薯LEA_2基因家族在GA（A）与JA（B）激素处理下的表达模式分析

Fig. 7 Expression patterns analysis of the potato LEA_2 gene family under GA (A) and JA (B) hormone treatments

图8 马铃薯LEA_2基因不同成员响应低温、GA与JA的表达模式不同字母代表P0.05差异显著，数值为平均值±标准误（n=3），下同

Fig. 8 Expression patterns of different potato LEA_2 gene members in response to low temperature, GA, and JADifferent letters indicate significant differences at P0.05. Values are presented as means ± SE (n=3), the same below

图9 马铃薯StLEA37基因在烟草叶片中的亚细胞定位及基因表达分析

Fig. 9 Subcellular localization and gene expression analysis of the potato StLEA37 gene in tobacco leaves

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马铃薯LEA_2基因家族鉴定及响应低温与激素功能分析

Identification and Functional Analysis of the LEA_2 Gene Family in Potato in Response to Cold Stress and Hormones

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