MT-III基因在不同海拔地区藏羊及其脑组织不同部位的差异表达研究

摘要/Abstract

摘要： 为了了解金属硫蛋白-3(Metallothionein-3，MT-III)基因在不同海拔地区饲养的藏羊脑组织及脑组织不同部位间的表达差异，随机选取高海拔地区(青海河南县)饲养的成年雄性藏羊6只和低海拔地区(甘肃临洮县)饲养的成年雄性藏羊3只，屠宰后分别在脑组织的颞叶、顶叶、延髓、小脑及松果体各部位取样，利用实时荧光定量PCR法研究MT-III基因在藏羊脑组织及其不同部位的相对表达量。结果显示，MT-III基因在藏羊脑组织各部均有表达，但不同部位相对表达量有差异；藏羊松果体中MT-III基因的相对表达量极显著高于其它部位(P<0.01)；饲养在高海拔地区藏羊的脑组织的颞叶、顶叶和延髓中，MT-III基因的相对表达量均极显著高于低海拔地区饲养的藏羊(P<0.01)。MT-III是在藏羊脑组织不同部位普遍表达的一种蛋白，其在颞叶、顶叶和延髓较高的表达量可能与该区域脑细胞对低氧及高辐射的耐受性有关。

关键词: MT-III, 基因表达, 藏羊, 脑组织不同部位, 不同海拔地区

Abstract: The study was set out to find the differences of Metallothionein-3(Metallothionein-3, MT-III)gene of Tibetan sheep in different altitudes areas and its expression differences in different parts of the brain tissue. Randomly selected 6 Tibetan rams from high altitude areas(Henan county, Qinghai)and 3 Tibetan rams form low altitude areas(Lintao county, Gansu), sampled various parts of the temporal lobe, parietal lobe, medulla, cerebellum and pineal gland in the brain tissue after slaughter, respectively, and analyzed the relative expression levels of MT-III gene at the brain tissue and other different parts of Tibetan sheep in different altitude areas by real-time PCR method. MT-III gene were expressed in various parts of brain tissue of Tibetan sheep, but there are differences of the relative expression levels in different parts；the relative expression of MT-III gene in pineal gland was significantly higher than that of other parts(P <0.01)；at high altitude, the relative expression of MT-III gene in the temporal lobe, parietal lobe and medulla were significantly higher than those of Tibetan sheep at low altitude areas(P <0.01). MT-III is a protein that expressed in different parts of brain tissue of Tibetan sheep, its high expression in the temporal lobe, parietal lobe and medulla will cause the brain cells in this region enhanced tolerance to hypoxia and high radiation.

Key words: MT-III, Gene expression, Tibetan-sheep Different, Parts of the brain tissue, Different altitudes areas

王佳丽, 王欣荣, 赵生国, 王建福, 吴建平. MT-III基因在不同海拔地区藏羊及其脑组织不同部位的差异表达研究[J]. 生物技术通报, 2014, 0(5): 112-116.

Wang Jiali, Wang Xinrong, Zhao Shengguo, Wang Jianfu, Wu Jianping. Comparison of Metallothionein-3 Gene Expression Levels in Different Brain Parts of Tibetan-sheep Between Different Altitudes Areas[J]. Biotechnology Bulletin, 2014, 0(5): 112-116.

参考文献

[1] 赵有璋. 羊生产学[M]. 北京：中国农业出版社, 2007：110-112.
[2] Moffatt P, Seguin C. Express of the gene encoding metallothionein-3 in organs of the reproductive system[J]. DNA Cell Biol, 1998, 17：501-510.
[3] Wright J, George S, Martinez Lara E, et al. Levels of cellular glutathione and metallothionein affect the toxicity of oxidative stressors in an established carp cell line[J]. Mar Environ Res, 2000, 50：50-38.
[4] 杨联. 牦牛金属硫蛋白(MTs)-III/-IV分子特性及MT-III表达研究[D]. 兰州：甘肃农业大学, 2008：16-24.
[5] Miyazaki I, Asanuma M, Higashi Y, et al. Age-related changes in expression of metallothionein-III in rat brain[J]. Neuroscience Research, 2002：323-333.
[6] Ishihara K, Hirano T. IL-6 in autoimmune disease and chronic inflammatory Proliferative disease[J]. Cytokine Growth Factor Rev, 2002, 13：357-368.
[7] Ghazi IEI, Martin BL, Armitage IM. Metallothionein-3 is a component of amutiprotein complex in the mouse brain[J]. Exp Biol Med, 2006, 231：1500-1506.
[8] Miles AT, Hawksworth GM, Beattie JH, et al. Induction, regulation, degradation, and biological significance of mammalian metallothion-eins[J]. Crit Rev Bioehem Mol Biol, 2000, 35：35-70.
[9] Palmiter RD, Findley SD, Whitmore TE, et al. MT-III, a brain-specific member of the metallothionein gene family[J]. PNAS, 1992, 14：6333-6337.
[10] Donghoul K, Hae KE, Changmee K, et al. Differential regulation of metallothionein-I, II, and III mRNA expression in the rat brain following kainic acid treatment[J]. Biol Trace Elem Res, 2000, 74(1)：23-30.
[11] Giralta M, Carrascoa J, Penkowa M, et al. Astrocyte-targeted expression of interleukin-3 and interferon-α causes region-specific changes in metallothionein expression in the brain[J]. Experimental Neurology, 2001, 168(2)：334-346.
[12] Ono Sl, Cai L, Koropatnick J, et al. Radiation exposure does not alter metallothionein III isoform expression in mouse brain[J]. Biol Trace Elem Res, 2000, 74(1)：23-30.
[13] Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method[J]. Nature Protocols, 2008, 3：1101-1108 .
[14] Yu L, Domann FE. Rapid and direct quantitative RT-PCR method to measure promoter activity[J]. Biotechnology Progress, 2006, 22(5)：1461-1463.
[15] Ebadi M, Iversen PL, Hao R, et al. Expression and regulation of brain metallothionein[J]. Neurochem, 1995, 27：1-2.
[16] Cuajungco MP, Lees GJ. Zine metabolism in the brain：relevance to human neurodegenerative disorders[J]. Neurobiol Dis, 1997, 4：137-169.
[17] Lee SJ, Koh JY. Roles of zinc and metallothionein-3 in oxidative stress-induced lysosomal dysfunction, cell death and autophagy in neurons and astrocytes[J]. Mol Brain, 2010, 3(1)：30-39.
[18] Lee SJ, Park MH, Kim HJ, et al. Metallothionein-3 regulates lysosomal function in cultured astrocytes under both normal and oxidative conditions[J]. Glia, 2010, 58(10)：1186-1196.
[19] Durand J, Meloni G, Talmard C, et al. Zinc release of Zn7-metallothionein-3 induces fibrillar type amyloid-β aggregates[J].Metallomics, 2010, 2：741-744.
[20] Howells C, West AK, Chung RS. Neuronal growth-inhibitory factor(metallothionein-3)：evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain[J]. FEBS, 2010, 277(14)：2931-2939.
[21] Bailey MJ, Coon SL, Carter DA, et al. Night/day changes in pineal expression of >600 genes central role of adrenergic/cAMP signaling[J]. Biological Chemistry, 2009, 284：7606-7622.
[22] Kleina DC, Baileyb MJ, Carterc DA, et al. Pineal function：impact of microarray analysis[J]. Molecular and Cellular Endocrinology, 2010, 314(2)：170-183.