纳米硒抗胰岛β细胞凋亡作用与机制的研究

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.035

生物技术通报 ›› 2016, Vol. 32 ›› Issue (11): 271-277.doi: 10.13560/j.cnki.biotech.bull.1985.2016.11.035

纳米硒抗胰岛β细胞凋亡作用与机制的研究

饶磊¹, 饶敏², 仇红红¹, 李红辉¹, 刘静¹, 庄满娇³

1. 成都医学院生物医学系,成都 610500;
2. 蚌埠94565部队卫生队,蚌埠 233000;
3. 广州大学生命科学学院,广州 510006

收稿日期:2016-07-04 出版日期:2016-11-25 发布日期:2016-11-11
作者简介:饶磊,男,博士研究生,讲师,研究方向：纳米药物;E-mail：591617735@qq.com
基金资助:
四川养老中心项目（YLZBZ1517）

Effect and Mechanism of Selenium Nanoparticle Against Islet β Cell Apoptosis

RAO Lei¹, RAO Min², QIU Hong-hong¹, LI Hong-hui¹, LIU Jing¹, ZHUANG Man-jiao³

1. Biomedical Science,Chengdu Medical College,Chengdu 610500;
2. Bengbu 94565 Troops Medical Corp,Bengbu 233000;
3. College of Life Science,Guangzhou University,Guangzhou 510006

Received:2016-07-04 Published:2016-11-25 Online:2016-11-11

摘要/Abstract

摘要： 旨在研究纳米硒（SeNPs）抗二型糖尿病胰岛β细胞凋亡的作用及机制。通过电子显微镜,纳米粒度仪等技术方法对SeNPs的表征进行鉴定,并运用相关分子生物学技术验证其对于胰岛β细胞凋亡模型的保护作用及机制。结果表明,壳聚糖（CS）修饰后的SeNPs表面电荷增加到24.8 mv,其保存期延长到100 d以上。另外,细胞学实验表明浓度在2 μmol/L以下SeNPs不具有毒性,38.1 nmol/L SeNPs可通过激活硫氧还蛋白还原酶（TrxR）活性,从而减轻胞内氧化应激（ROS）水平,最终使胰岛β凋亡模型细胞存活率提高了31.75%。动物学实验也表明SeNPs能抗胰岛β细胞凋亡。因此,SeNPs可有效减轻二型糖尿病中胰岛β细胞凋亡,对于二型糖尿病具有潜在的治疗价值。

关键词: 纳米硒, 二型糖尿病, 氧化应激, 分子机制

Abstract: The purpose of this research was to study the effect and molecular mechanism of selenium nanoparticles（SeNPs）in the treatment of islet β cell apoptosis in type 2 diabetes mellitus. Electron microscope and nanoparticle size analyzer were used to identify SeNPs surface morphology,and relevant molecular biological techniques were used to validate the protection effect and mechanism of SeNPs on islet β cells apoptotic model. Results showed that the surface charge of selenium nanoparticle decorated by chitosan（CS）increased to 24.8 mv,and its storage time was extended to over 100 days. Cytology results showed that SeNPs under 2 μmol/L had no toxicity. Meanwhile,SeNPs in 38.1 nmol/L activated TrxR activity and reduced intracellular ROS level,which led to apoptotic model cells survival rate significantly increased 31.75%. Furthermore,the animal experiments also verified that SeNPs could resist the apoptosis of islet cell β. In conclusion,SeNPs may eliminate the apoptosis of islet cell β in type 2 diabetes mellitus,which shows a potential therapeutic activity in the treatment of type 2 diabetes.

Key words: selenium nanoparticle, type 2 diabetes mellitus, oxidative stress, molecular mechanism

饶磊, 饶敏, 仇红红, 李红辉, 刘静, 庄满娇. 纳米硒抗胰岛β细胞凋亡作用与机制的研究[J]. 生物技术通报, 2016, 32(11): 271-277.

RAO Lei, RAO Min, QIU Hong-hong, LI Hong-hui, LIU Jing, ZHUANG Man-jiao. Effect and Mechanism of Selenium Nanoparticle Against Islet β Cell Apoptosis[J]. Biotechnology Bulletin, 2016, 32(11): 271-277.

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纳米硒抗胰岛β细胞凋亡作用与机制的研究

Effect and Mechanism of Selenium Nanoparticle Against Islet β Cell Apoptosis

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