牛的基因编辑技术研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0413

摘要/Abstract

摘要： 基因编辑技术是对基因组进行定点修饰的一种新兴生物技术,主要包括锌指核酸酶（ZFNs）技术、转录激活因子样效应物核酸酶（TALENs）技术以及最新的CRISPR/Cas9技术,正在动植物育种、生物制药、基因治疗等方面发挥前所未有的影响力。本文总结了基因打靶技术及各种基因编辑技术的发展简史,重点介绍了基因编辑技术在抗病、改善奶品质、提高瘦肉率和去角等牛新品种培育领域的研究进展。最后提出建立基因编辑农业动物监管体系的建议,以期加快我国基因工程动物产业化。

关键词: 基因编辑技术, ZFNs, TALENs, Cas9, 牛品质改良, 基因编辑生物的监管政策

Abstract: Gene editing technology is an emerging biotechnology for site-directed modification of the genome,including zinc-finger nuclease（ZFN）technology,transcriptional activator-like effect nuclease（TALEN）technology and the recently developed CRISPR/Cas9 technology,which has exerted unprecedented influence in the breeding of the animal and plant,biopharmaceuticals and gene therapy,etc. In this review,we summarize the brief history of gene targeting and gene editing technology,and highlight the research progress of gene editing technology in the fields of the resistance of disease,the improvement of milk quality and lean meat percentage,and the cultivation of new varieties such as hornless cattle. Finally,we also propose the establishment of a gene editing plants and animals’ supervision system to speed up the industrialization of genetic engineering animals in China.

Key words: gene editing technology, ZFNs, TALENs, Cas9, cow genetic improvement, gene editing biology regulatory policy

汤波,孙照霖,戴蕴平. 牛的基因编辑技术研究进展[J]. 生物技术通报, 2018, 34(5): 41-47.

TANG Bo, SUN Zhao-lin, DAI Yun-ping. Research Progress of Gene Editing Technology on Cattle[J]. Biotechnology Bulletin, 2018, 34(5): 41-47.

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