生物技术通报 ›› 2021, Vol. 37 ›› Issue (10): 26-33.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0588

• 研究报告 • 上一篇    下一篇

盐胁迫条件下‘巴麻火麻’内生真菌的分离鉴定与多样性分析

李娥1(), 黄勇1, 孟园园1, 李璇2, 杜光辉2(), 刘飞虎1,2()   

  1. 1.云南大学生命科学学院,昆明 650091
    2.云南大学农学院,昆明 650091
  • 收稿日期:2020-05-14 出版日期:2021-10-26 发布日期:2021-11-12
  • 作者简介:李娥,女,硕士研究生,研究方向:植物耐盐性及植物内生真菌;E-mail: 2049829995@qq.com
  • 基金资助:
    国家麻类产业技术体系建设专项资金(CARS-16-E15);国家自然科学基金项目(31871671);云南大学第十届研究生科研创新项目(2018194)

Isolation and Identification of the Endophytic Fungi of‘Bama hemp’ Under Salt Stress and Its Diversity Analysis

LI E1(), HUANG Yong1, MENG Yuan-yuan1, LI Xuan2, DU Guang-hui2(), LIU Fei-hu1,2()   

  1. 1. College of Life Sciences,Yunnan University,Kunming 650091
    2. School of Agriculture,Yunnan University,Kunming 650091
  • Received:2020-05-14 Published:2021-10-26 Online:2021-11-12

摘要:

旨在研究‘巴麻火麻’不同浓度盐处理下植物内生真菌动态变化规律,并筛选到与缓解盐胁迫密切相关的内生真菌。以前期筛选的耐盐工业大麻品种‘巴麻火麻’为研究材料,通过不同浓度(0、100、200和300 mmol/L)NaCl盐处理,利用传统方法对大麻内生真菌进行分离纯化,结合形态学和rDNA-ITS序列分析对菌种进行鉴定。1 400个大麻组织片中共分离到543株真菌,隶属于14个科18个属44个种。研究发现,盐胁迫处理影响大麻内生真菌群落组成,不同浓度NaCl处理下分离到的内生真菌不同,各处理间相似性系数均小于0.5。随着NaCl处理浓度的增加,内生真菌的丰富度、多样性指数、分离率均呈先降后升的趋势。其中,在200 mmol/L NaCl处理下,丰富度和多样性指数最低,而100 mmol/L NaCl处理下,分离率达到最低。在所有的处理中,均分离得到与抗逆性密切相关的毛壳菌(Chaetomium globosum)、哈茨木霉(Trichoderma harzianum)、炭垫菌(Nemania diffusa)、枝孢霉(Cladosporium)、炭团菌(Annulohypoxylon stygium)等。

关键词: 工业大麻, 盐胁迫, 内生真菌

Abstract:

The aim is to study the dynamic changes of plant endophytic fungi under different concentrations of salt treatment in “ Bama hemp ”,and to screen endophytic fungi closely related to alleviating salt stress. The salt tolerant industrial cannabis variety ‘Bama hemp’ screened in the previous period was as study material. It was treated with different concentrations(0,100,200,and 300 mmol/L)of NaCl salt. The traditional method was used to separate and purify endophytic fungi. The strains were identified by rDNA-ITS sequence analysis combined with morphology. A total of 543 fungi were isolated from 1 400 cannabis tissue tablets,belonging to 44 families,18 genera and 44 species. It was found that salt stress treatment affected the composition of endophytic fungi in cannabis. The endophytic fungi isolated under different concentrations of NaCl were different,and the similarity coefficient was < 0.5. With the increase of NaCl concentration,the richness,diversity index and isolation rate of endophytic fungi decreased first and then increased. Among them,the richness and diversity index were the lowest under the treatment of 200 mmol/L NaCl,while the separation rate was the lowest under 100 mmol/L NaCl treatment. In all treatments,Chaetomium globosum,Trichoderma harzianum,Nemania diffusa,Cladosporium,Annulohypoxylon stygium,et al. were isolated,which were closely related to stress resistance.

Key words: industrial hemp, salt stress, endophytic fungi