生物技术通报 ›› 2023, Vol. 39 ›› Issue (12): 99-108.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0580

• 技术与方法 • 上一篇    下一篇

RNAi技术在寄生蜂中的应用研究进展

张龙喜1(), 吕琳1, 张欢欢2(), 周金成1, 车午男1, 董辉1()   

  1. 1.沈阳农业大学植物保护学院,沈阳 110866
    2.西藏自治区农牧科学院蔬菜研究所,拉萨 850032
  • 收稿日期:2023-06-16 出版日期:2023-12-26 发布日期:2024-01-11
  • 通讯作者: 张欢欢,助理研究员,研究方向:病虫害生物防治;E-mail: 1349282933@qq.com
    董辉,博士,教授,研究方向:害虫生物防治;E-mail: biocontrol@163.com
  • 作者简介:张龙喜,硕士研究生,研究方向:害虫生物防治;E-mail: 1483323869@qq.com
  • 基金资助:
    国家自然科学基金项目(32172476);西藏自治区中央引导地方项目(XZ202301YD0042C);辽宁省教育厅科学研究经费项目(LJKZ0646)

Research Progress in the Application of RNAi Technology in Parasitoid Wasps

ZHANG Long-xi1(), LYU Lin1, ZHANG Huan-huan2(), ZHOU Jin-cheng1, CHE Wu-nan1, DONG Hui1()   

  1. 1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866
    2. Institute of Vegetable, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850032
  • Received:2023-06-16 Published:2023-12-26 Online:2024-01-11

摘要:

RNA干扰技术问世至今20余年,已被广泛用于基因功能研究。膜翅目寄生蜂是农林害虫防治领域重要的天敌昆虫资源。RNAi技术对靶基因的昆虫沉默效果依赖于dsRNA的递送效率。寄生蜂幼期通过摄取寄主昆虫营养完成其生长发育,并与寄主昆虫发生相互作用,给RNAi技术的实施带来挑战。目前,RNAi技术已在丽蝇蛹集金小蜂(Nasonia vitripennis)、中红侧沟茧蜂(Microplitis mediator)、菜蛾盘绒茧蜂(Cotesia vestalis)、蝶蛹金小蜂(Pteromalus puparum)、斑痣悬茧蜂(Meteorus pulchricornis)等至少13种寄生蜂中开展。本文归纳了RNAi技术在寄生蜂基因调控方面的研究进展,并总结了在寄生蜂类群中影响RNAi效率的因素,以期为RNAi技术在寄生蜂类群中的应用提供参考。

关键词: 寄生蜂, RNA干扰, 分子机制, RNAi效率

Abstract:

RNA interference technology has been widely used for investigating functions of genes for more than 20 years since its inception. In Hymenoptera, parasitoid wasps are a group of important natural enemy against pests in agriculture and forestry. The inhibition of target gene expression relies on the delivery of dsRNA. However, the parasitoids develop on host insects, and interact with their hosts. This biological characteristic makes it challenging to conduct RNAi technology. The RNAi technology has already been conducted in the studies involved the molecular mechanisms of at least 13 species of parasitoid wasps, e.g. Nasonia vitripennis, Microplitis mediator, Cotesia vestalis, Pteromalus puparum, and Meteorus pulchbernis. This study reviewed the studies of the molecular mechanisms by using RNAi method, and summarized the factors influencing RNAi efficiency in parasitoid wasps, aiming to provide the reference for application of RNAi technology in parasitoid wasps.

Key words: parasitoid wasps, RNA interference, molecular mechanism, RNAi efficiency